PINVIALEG

Portable microfluidic-based device for in situ detection of viable legionella

Coordinatore	ONDOAN S COOP LTDA    more  Organization address city: Zamudio postcode: 48170 contact info Titolo: Mr. Nome: Pedro Cognome: Gurrutxaga Email: send email Telefono: +34 94 452 2313 Fax: +34 94 452 1047
Nazionalità Coordinatore	Spain [ES]
Sito del progetto	http://www.pinvialeg.eu
Totale costo	1˙667˙371 €
EC contributo	1˙134˙495 €
Programma	FP7-SME Specific Programme "Capacities": Research for the benefit of SMEs
Code Call	FP7-SME-2010-1
Funding Scheme	BSG-SME
Anno di inizio	2010
Periodo (anno-mese-giorno)	2010-11-01   -   2012-12-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	ONDOAN S COOP LTDA  Organization address city: Zamudio postcode: 48170 contact info Titolo: Mr. Nome: Pedro Cognome: Gurrutxaga Email: send email Telefono: +34 94 452 2313 Fax: +34 94 452 1047	ES (Zamudio)	coordinator	297˙397.00
2	MK FLUIDICS OY  Organization address address: Mataratie 2 A 1 city: Oulu postcode: 90580 contact info Titolo: Mr. Nome: Markku Tapani Cognome: Känsäkoski Email: send email Telefono: +358 4054 50903 Fax: +358 4054 50903	FI (Oulu)	participant	319˙325.00
3	Q-BIOANALYTIC GMBH  Organization address address: FISCHKAI 1 city: BREMERHAVEN postcode: 27572 contact info Titolo: Dr. Nome: Boris Cognome: Oberheitmann Email: send email Telefono: +49 471 4832 442 Fax: +49 471 4832 458	DE (BREMERHAVEN)	participant	261˙659.20
4	INNOSIEVE DIAGNOSTICS BV  Organization address address: CELSIUSLAAN 5 city: VLIJMEN postcode: 5251 ZB contact info Titolo: Dr. Nome: Michel Cognome: Klerks Email: send email Telefono: +31 646717500	NL (VLIJMEN)	participant	247˙513.50
5	AYUNTAMIENTO DE BILBAO  Organization address address: URIBITARTE 18-4 DCHA city: BILBAO postcode: 48001 contact info Titolo: Ms. Nome: Marina Cognome: Lizarralde Email: send email Telefono: +34 94 4204964 Fax: +34 94 4422204	ES (BILBAO)	participant	8˙600.03
6	FUNDACION GAIKER  Organization address address: Parque Tecnologico de Zamudio, Edificio 202 city: ZAMUDIO postcode: 48170 contact info Titolo: Mr. Nome: Francisco Cognome: Garcia Email: send email Telefono: +34 94 600 2323 Fax: +34 94 600 2324	ES (ZAMUDIO)	participant	0.00
7	STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK  Organization address address: Costerweg 50 city: WAGENINGEN postcode: 6701BH contact info Titolo: Mr. Nome: Ruben Cognome: Van Deursen Email: send email Telefono: +31 317 482985 Fax: +31 317 483011	NL (WAGENINGEN)	participant	0.00
8	TEKNOLOGIAN TUTKIMUSKESKUS VTT  Organization address address: TEKNIIKANTIE 4 A city: ESPOO postcode: 02044 VTT contact info Titolo: Ms. Nome: Päivi Cognome: Heimala Email: send email Telefono: +358 20 722 6637 Fax: +358 20 722 7012	FI (ESPOO)	participant	0.00

Mappa

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 Obiettivo del progetto (Objective)

'Legionella outbreaks have great sanitary, economic and social implications. Diagnostic method use to prevent and control Legionella is bacteria culture. Available detection kits are based on DNA technology, unable to discriminate between viable and non-viable bacteria. On the contrary, RNA is widely used as a viable bacteria indicator. Although molecular based techniques, as PCR and qPCR, have become essential tools in genetic analyses due to its high sensitivity and accuracy, they show drawbacks as time-consuming and expensive. Recent development of integrated microfluidic systems has allowed time, volume and analysis cost reduction. An important application of microfluididc systems is the ability to efficiently process raw samples and perform the required analytical assays “on-chip”. There is no portable system to detect viable Legionella on-site in the market. This device will avoid sample pre-treatment therefore reducing data acquisition. Moreover, rapid result gathering will allow implementing a rapid plan of action for bacteria eradication. The project aims to develop a portable microfluidic device composed of a filtration and bacteria collection system and a disposable card (containing a microfluidic chamber for nucleic acids concentration, a microfluidic chamber for RNA amplification through NASBA, and a detection system based on a NALFIA strip). An essential part of PINVIALEG is the development of a portable device where the filtration system is integrated to a microfluidic based cartridge. Thus, the whole process of Legionella detection (from sample collection to results) can be performed on site. NASBA is an isothermal process, facilitating portable equipment production. Development of a NALFIA strip able to detect NASBA products as a reading system will make the device very simple to use by non-qualified personnel. A new innovative tool will be produced to detect viable Legionella on-site, with rapid result acquisition and easy to use.'