TOTIPOTENCY2014
Dissecting the epigenetic control of totipotency.
Total Cost €
0EC-Contrib. €
0Partnership
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Project "TOTIPOTENCY2014" data sheet
The following table provides information about the project.
| Coordinator |
THE BABRAHAM INSTITUTE
Organization address contact info |
| Coordinator Country | United Kingdom [UK] |
| Project website | https://www.babraham.ac.uk/our-research/epigenetics/wolf-reik |
| Total cost | 183˙454 € |
| EC max contribution | 183˙454 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2014 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2016 |
| Duration (year-month-day) | from 2016-01-01 to 2017-12-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | THE BABRAHAM INSTITUTE | UK (CAMBRIDGE) | coordinator | 183˙454.00 |
Map
Project objective
One of the most critical epigenetic and chromatin remodelling processes in mammalian development occurs shortly after fertilization restoring totipotency. Due to limited cell numbers and lack of experimentally tractable systems, the mechanisms and regulation of this developmental stage are poorly understood. This proposal will provide important mechanistic insights into the epigenetic control of early embryonic gene expression, and its relevance to somatic reprogramming.
Murine embryonic stem cells (ESCs) contain a rare sub-population of early embryonic like (EEL) cells expressing genes normally restricted to the pre-implantation embryo, with enhanced extra-embryonic differentiation capability. Firstly, using novel single-cell technologies, I will characterize the EEL cells in detail, validating them as an in vitro model of pre-implantation development. On-going research in the host lab that I am involved in has identified an epigenetic enhancer that expands this EEL sub-population. Through bioinformatic analysis of pre-implantation transcriptome data, I have shortlisted 34 epigenetic and chromatin-associated proteins, including the previously identified factor, which will be systematically screened to identify new enhancers of totipotency. Validated EEL regulators, including two additional newly confirmed factors, will be analysed mechanistically determining their interaction partners, interdependencies, and mode of action. Finally, the contribution of the totipotency regulators towards the efficiency and fidelity of somatic reprogramming will be determined, potentially improving the use of this technology for personalised gene therapy and regenerative medicine.
This state-of-the-art proposal uses innovative and novel cutting-edge technologies and combines the expertise of the researcher and the host. It has the potential for significant impact across several fields from epigenetics and stem cell biology to reprogramming and regenerative medicine.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2016 |
Mélanie A. Eckersley-Maslin, Valentine Svensson, Christel Krueger, Thomas M. Stubbs, Pascal Giehr, Felix Krueger, Ricardo J. Miragaia, Charalampos Kyriakopoulos, Rebecca V. Berrens, Inês Milagre, Jörn Walter, Sarah A. Teichmann, Wolf Reik MERVL/Zscan4 Network Activation Results in Transient Genome-wide DNA Demethylation of mESCs published pages: 179-192, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2016.08.087 |
Cell Reports 17/1 | 2019-06-14 |
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