NANOBUBBLE SIGNED
Laser-induced vapour nanobubbles for intracellular delivery of nanomaterials and treatment of biofilm infections
Total Cost €
0EC-Contrib. €
0Partnership
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Explore the words cloud of the NANOBUBBLE project. It provides you a very rough idea of what is the project "NANOBUBBLE" about.
Project "NANOBUBBLE" data sheet
The following table provides information about the project.
| Coordinator |
UNIVERSITEIT GENT
Organization address contact info |
| Coordinator Country | Belgium [BE] |
| Project website | http://www.ugent.be/fw/pharmaceutics/biochemphypharm/en/research/biophotonics/photoporation.htm |
| Total cost | 2˙236˙250 € |
| EC max contribution | 2˙236˙250 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2014-CoG |
| Funding Scheme | ERC-COG |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-09-01 to 2020-08-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | UNIVERSITEIT GENT | BE (GENT) | coordinator | 1˙980˙000.00 |
| 2 | UNIVERSITE DE LILLE | FR (LILLE) | participant | 256˙250.00 |
| 3 | UNIVERSITE DES SCIENCES ET TECHNOLOGIES DE LILLE - LILLE I | FR (VILLENEUVE D'ASCQ) | participant | 0.00 |
Map
Project objective
Lasers have found widespread application in medicine, such as for photothermal therapy. Gold nanoparticles (AuNPs), are often used as enhancers of the photothermal effect since they can efficiently absorb laser light and convert it into thermal energy. When absorbing intense nano- or picosecond laser pulses, AuNPs can become extremely hot and water vapor nanobubbles (VNBs) can emerge around these particles in tissue. A VNB will expand up to several hundred nm until the thermal energy from the AuNP is consumed, after which the bubble violently collapses, causing mechanical damage to neighbouring structures. In this project the aim is to make use of the disruptive mechanical force of VNBs to enable highly controlled and efficient delivery of macromolecules and nanoparticles in cells and biofilms. First, optical set-ups and microfluidics devices will be developed for high-throughput treatment of cells and biofilms. Second, VNBs will be used to achieve efficient cytosolic delivery of functional macromolecules in mammalian cells by cell membrane perforation or by inducing endosomal escape of endocytosed nanomedicine formulations that are functionalized with AuNPs. These concepts will be applied to tumorigenesis research, generation of induced pluripotent stem cells and modulation of effector T-cells for adoptive T-cell anti-cancer therapy. Third, contrast nanoparticles for cell imaging will be delivered into the cytosol of mammalian cells through VNB induced cell membrane perforation. This will enable more reliable in vivo imaging of labelled cells, labelling of subcellular structures for time-lapse microscopy and intracellular biosensing. Finally, [... confidential...] laser-induced VNBs will be used [... confidential...] for improved eradication of biofilms. This concept will be applied to biofilm infections in dental root canals and chronic wounds.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2020 |
Jing Liu, Tim Hebbrecht, Toon Brans, Eef Parthoens, Saskia Lippens, Chengnan Li, Herlinde De Keersmaecker, Winnok H. De Vos, Stefaan C. De Smedt, Rabah Boukherroub, Jan Gettemans, Ranhua Xiong, Kevin Braeckmans Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation published pages: 485-495, ISSN: 1998-0124, DOI: 10.1007/s12274-020-2633-z |
Nano Research 13/2 | 2020-04-15 |
| 2018 |
Gaëlle Houthaeve, Ranhua Xiong, Joke Robijns, Bert Luyckx, Yasmine Beulque, Toon Brans, Coen Campsteijn, Sangram K. Samal, Stephan Stremersch, Stefaan C. De Smedt, Kevin Braeckmans, Winnok H. De Vos Targeted Perturbation of Nuclear Envelope Integrity with Vapor Nanobubble-Mediated Photoporation published pages: 7791-7802, ISSN: 1936-0851, DOI: 10.1021/acsnano.8b01860 |
ACS Nano 12/8 | 2020-04-15 |
| 2016 |
Ranhua Xiong, Roosmarijn E. Vandenbroucke, Katleen Broos, Toon Brans, Elien Van Wonterghem, Claude Libert, Jo Demeester, Stefaan C. De Smedt, Kevin Braeckmans Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability published pages: 12982, ISSN: 2041-1723, DOI: 10.1038/ncomms12982 |
Nature Communications 7 | 2019-06-06 |
| 2016 |
Ranhua Xiong, Freya Joris, Sayuan Liang, Riet De Rycke, Saskia Lippens, Jo Demeester, Andre Skirtach, Koen Raemdonck, Uwe Himmelreich, Stefaan C. De Smedt, Kevin Braeckmans Cytosolic Delivery of Nanolabels Prevents Their Asymmetric Inheritance and Enables Extended Quantitative in Vivo Cell Imaging published pages: 5975-5986, ISSN: 1530-6984, DOI: 10.1021/acs.nanolett.6b01411 |
Nano Letters 16/10 | 2019-06-06 |
| 2016 |
Ranhua Xiong, Sangram Keshari Samal, Jo Demeester, Andre G. Skirtach, Stefaan C. De Smedt, Kevin Braeckmans Laser-assisted photoporation: fundamentals, technological advances and applications published pages: 596-620, ISSN: 2374-6149, DOI: 10.1080/23746149.2016.1228476 |
Advances in Physics: X 1/4 | 2019-06-06 |
| 2017 |
Ranhua Xiong, Claire Drullion, Peter Verstraelen, Jo Demeester, Andre G. Skirtach, Corinne Abbadie, Winnok H. De Vos, Stefaan C. De Smedt, Kevin Braeckmans Fast spatial-selective delivery into live cells published pages: 198-204, ISSN: 0168-3659, DOI: 10.1016/J.JCONREL.2017.09.033 |
Journal of Controlled Release 266 | 2019-06-06 |
| 2018 |
Ranhua Xiong, Peter Verstraelen, Jo Demeester, Andre G. Skirtach, Jean-Pierre Timmermans, Stefaan C. De Smedt, Winnok H. De Vos, Kevin Braeckmans Selective Labeling of Individual Neurons in Dense Cultured Networks With Nanoparticle-Enhanced Photoporation published pages: Article 80, ISSN: 1662-5102, DOI: 10.3389/fncel.2018.00080 |
Frontiers in Cellular Neuroscience 12 | 2019-04-18 |
| 2018 |
Lotte Vermeulen, Juan Fraire, Laurens Raes, Ellen De Meester, Sarah De Keulenaer, Filip Van Nieuwerburgh, Stefaan De Smedt, Katrien Remaut, Kevin Braeckmans Photothermally Triggered Endosomal Escape and Its Influence on Transfection Efficiency of Gold-Functionalized JetPEI/pDNA Nanoparticles published pages: 2400, ISSN: 1422-0067, DOI: 10.3390/ijms19082400 |
International Journal of Molecular Sciences 19/8 | 2019-04-18 |
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