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Opendata, web and dolomites

FASTR 3D SIM SIGNED

Focus on Advancing Spatial and Temporal Resolution of 3D Structured Illumination Microscopy

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

FASTR 3D SIM project word cloud

Explore the words cloud of the FASTR 3D SIM project. It provides you a very rough idea of what is the project "FASTR 3D SIM" about.

cell advancing combination live resolved 150 microscopy candidate observe reconstruction illumination speed fast scales times extreme optical spatial resolution dynamics limit signaling imaging structuring scaffolding fluctuation extended rate fluorescence applicable microscope question captured dedecker sofi specialty cells host points acute sims surpass tools photo reduce er improvement labels length realized sr focal below ideal 3d structured damage ensures cellular nm revolutionary rapid 25 truly employed fastr create algorithms 100nm fairsim levels video code org super sim capture compatible approx toxicity mitochondrial repeatable optics images 2x biological fold temporal enhancement tackling interactions fluorescent light lab fastest nature linear quicker

Project "FASTR 3D SIM" data sheet

The following table provides information about the project.

Coordinator	KATHOLIEKE UNIVERSITEIT LEUVEN Organization address address: OUDE MARKT 13 city: LEUVEN postcode: 3000 website: www.kuleuven.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Belgium [BE]
Project website	http://fairsim.org
Total cost	160˙800 €
EC max contribution	160˙800 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2016
Funding Scheme	MSCA-IF-EF-ST
Starting year	2017
Duration (year-month-day)	from 2017-11-06 to 2019-11-05

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	KATHOLIEKE UNIVERSITEIT LEUVEN KATHOLIEKE UNIVERSITEIT LEUVEN Organization address address: OUDE MARKT 13 city: LEUVEN postcode: 3000 website: www.kuleuven.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	BE (LEUVEN)	coordinator	160˙800.00

Map

Project objective

FASTR 3D SIM: Focus on Advancing the Spatial and Temporal Resolution of 3D Structured Illumination Microscopy

Super-resolved structured illumination microscopy (SR-SIM) is an important method to surpass the resolution limit in fluorescence microscopy. SR-SIM is compatible with many fluorescent labels and ensures low photo-damage, making it an ideal candidate for live-cell imaging. However, to be truly applicable, very fast 3D imaging has to be realized, as the cellular structuring is 3D in nature and fast dynamics have to be captured. In addition, many biological processes occur at length scales below 100nm, beyond SR-SIMs two-fold resolution improvement.

I will develop an advanced 3D SR-SIM microscope able to capture 3D images at video rate, i.e., 25 3D images per second, 10 times quicker than the fastest currently available systems. The extreme speed-up is achieved by a unique multi-focal optics approach available at the host institute, and will also reduce the photo-toxicity to the sample. My SIM reconstruction code (www.fairsim.org) will be extended to 3D imaging, and include new algorithms tackling low light levels and strong out-of-focus light.

I will also develop a revolutionary combination of SIM with super-resolution optical fluctuation imaging (SOFI), a specialty of the Dedecker lab. SOFI is employed to create non-linear effects, allowing SIM to surpass its 2x resolution enhancement. This enables rapid and repeatable 3D imaging of cells with a spatial resolution approx. 60 nm in x,y and 150 nm in z.

I will apply the tools to an acute biological question, the dynamics of mitochondrial and ER interactions, used by the cell as signaling scaffolding points. Here both the temporal and spatial resolution of the proposed system is required to observe the very fast dynamics.

Publications

List of publications.
year	authors and title	journal	last update
2019	Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam DuwÃ©, Wolfgang HÃ¼bner, Thomas Huser, Peter Dedecker, Marcel MÃ¼ller Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI) published pages: 44001, ISSN: 2515-7647, DOI: 10.1088/2515-7647/ab36ae	Journal of Physics: Photonics 1/4	2020-04-24
2019	Andreas Markwirth, Mario Lachetta, Viola MÃ¶nkemÃ¶ller, Rainer Heintzmann, Wolfgang HÃ¼bner, Thomas Huser, Marcel MÃ¼ller Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-12165-x	Nature Communications 10/1	2020-04-24

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The information about "FASTR 3D SIM" are provided by the European Opendata Portal: CORDIS opendata.