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FASTR 3D SIM SIGNED

Focus on Advancing Spatial and Temporal Resolution of 3D Structured Illumination Microscopy

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

FASTR 3D SIM project word cloud

Explore the words cloud of the FASTR 3D SIM project. It provides you a very rough idea of what is the project "FASTR 3D SIM" about.

focal 150 extreme code repeatable compatible employed tackling advancing cell labels temporal org host linear scales specialty microscope damage resolved optics light 2x length revolutionary structuring combination photo optical toxicity scaffolding interactions extended surpass truly fluorescence imaging dynamics nature resolution rate reconstruction observe rapid sofi biological sims mitochondrial limit below er approx fold candidate quicker illumination improvement structured times fastest fairsim nm dedecker fastr create 3d question levels reduce images captured fluctuation 25 sim acute signaling ideal fluorescent spatial cells super live video ensures enhancement speed 100nm tools points realized cellular microscopy fast sr algorithms lab applicable capture

Project "FASTR 3D SIM" data sheet

The following table provides information about the project.

Coordinator	KATHOLIEKE UNIVERSITEIT LEUVEN Organization address address: OUDE MARKT 13 city: LEUVEN postcode: 3000 website: www.kuleuven.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Belgium [BE]
Project website	http://fairsim.org
Total cost	160˙800 €
EC max contribution	160˙800 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2016
Funding Scheme	MSCA-IF-EF-ST
Starting year	2017
Duration (year-month-day)	from 2017-11-06 to 2019-11-05

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	KATHOLIEKE UNIVERSITEIT LEUVEN KATHOLIEKE UNIVERSITEIT LEUVEN Organization address address: OUDE MARKT 13 city: LEUVEN postcode: 3000 website: www.kuleuven.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	BE (LEUVEN)	coordinator	160˙800.00

Map

Project objective

FASTR 3D SIM: Focus on Advancing the Spatial and Temporal Resolution of 3D Structured Illumination Microscopy

Super-resolved structured illumination microscopy (SR-SIM) is an important method to surpass the resolution limit in fluorescence microscopy. SR-SIM is compatible with many fluorescent labels and ensures low photo-damage, making it an ideal candidate for live-cell imaging. However, to be truly applicable, very fast 3D imaging has to be realized, as the cellular structuring is 3D in nature and fast dynamics have to be captured. In addition, many biological processes occur at length scales below 100nm, beyond SR-SIMs two-fold resolution improvement.

I will develop an advanced 3D SR-SIM microscope able to capture 3D images at video rate, i.e., 25 3D images per second, 10 times quicker than the fastest currently available systems. The extreme speed-up is achieved by a unique multi-focal optics approach available at the host institute, and will also reduce the photo-toxicity to the sample. My SIM reconstruction code (www.fairsim.org) will be extended to 3D imaging, and include new algorithms tackling low light levels and strong out-of-focus light.

I will also develop a revolutionary combination of SIM with super-resolution optical fluctuation imaging (SOFI), a specialty of the Dedecker lab. SOFI is employed to create non-linear effects, allowing SIM to surpass its 2x resolution enhancement. This enables rapid and repeatable 3D imaging of cells with a spatial resolution approx. 60 nm in x,y and 150 nm in z.

I will apply the tools to an acute biological question, the dynamics of mitochondrial and ER interactions, used by the cell as signaling scaffolding points. Here both the temporal and spatial resolution of the proposed system is required to observe the very fast dynamics.

Publications

List of publications.
year	authors and title	journal	last update
2019	Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam DuwÃ©, Wolfgang HÃ¼bner, Thomas Huser, Peter Dedecker, Marcel MÃ¼ller Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI) published pages: 44001, ISSN: 2515-7647, DOI: 10.1088/2515-7647/ab36ae	Journal of Physics: Photonics 1/4	2020-04-24
2019	Andreas Markwirth, Mario Lachetta, Viola MÃ¶nkemÃ¶ller, Rainer Heintzmann, Wolfgang HÃ¼bner, Thomas Huser, Marcel MÃ¼ller Video-rate multi-color structured illumination microscopy with simultaneous real-time reconstruction published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-12165-x	Nature Communications 10/1	2020-04-24

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