RECEPIANCE SIGNED
Molecular reconstitution of epigenetic centromere inheritance
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Project "RECEPIANCE" data sheet
The following table provides information about the project.
| Coordinator |
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Organization address contact info |
| Coordinator Country | Germany [DE] |
| Total cost | 2˙064˙583 € |
| EC max contribution | 2˙064˙583 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2014-ADG |
| Funding Scheme | ERC-ADG |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-08-01 to 2020-07-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV | DE (MUENCHEN) | coordinator | 2˙064˙583.00 |
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Project objective
Accurate segregation of the parental genome to the daughter cells during mitosis is essential for cell and organismal viability, protecting against cell death or cellular transformation. Kinetochores are crucial for accurate chromosome segregation because they link chromosomes to spindle microtubules. Kinetochores sprout from unique chromosomal loci named centromeres. In most eukaryotes, centromere specification does not rely on defined DNA sequences. Rather, centromere specification relies on epigenetic mechanisms, the most prominent of which is identified in the enrichment at centromeres of the histone H3 variant CENP-A. Deposition of new CENP-A at every cell cycle is vital for centromere maintenance through cell division. RECEPIANCE aims to dissect the molecular mechanism of CENP-A deposition through reductionist approach based on two main pillars, biochemical reconstitution and structural analysis.
The CENP-A deposition machinery consists of chromatin-remodelling factors, histone chaperones, and accessory factors, including the Mis18 complex, HJURP, RbAb46/48, the FACT complex, RSF, CHD1, and additional currently less characterized proteins. The CENP-A deposition machinery is recruited to centromeres in a cell cycle-regulated manner by inner kinetochore proteins associated with CENP-A, most notably CENP-C. The hypothesis inspiring this proposal is that the CENP-A deposition machinery operates on a unit containing at least one CENP-A nucleosome and a neighboring canonical H3 nucleosome, with the latter being the target for exchange with new CENP-A. Our ability to reconstitute the centromere-inner kinetochore interface puts us in an ideal position to study how the CENP-A deposition machinery is recruited to centromeres in vitro, dissecting the crucial binding interfaces and studying them biochemically and structurally. The ultimate goal of RECEPIANCE is to reconstitute the ATP-dependent exchange of H3 with CENP-A in vitro exclusively with recombinant proteins.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2017 |
Satyakrishna Pentakota, Keda Zhou, Charlotte Smith, Stefano Maffini, Arsen Petrovic, Garry P Morgan, John R Weir, Ingrid R Vetter, Andrea Musacchio, Karolin Luger Decoding the centromeric nucleosome through CENP-N published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.33442 |
eLife 6 | 2019-10-08 |
| 2018 |
Giuseppe Ciossani, Katharina Overlack, Arsen Petrovic, Pim J. Huis in \'t Veld, Carolin Koerner, Sabine Wohlgemuth, Stefano Maffini, Andrea Musacchio The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases published pages: 10084-10101, ISSN: 0021-9258, DOI: 10.1074/jbc.RA118.003154 |
Journal of Biological Chemistry 293/26 | 2019-10-08 |
| 2018 |
Marion E. Pesenti, Daniel Prumbaum, Philip Auckland, Charlotte M. Smith, Alex C. Faesen, Arsen Petrovic, Muriel Erent, Stefano Maffini, Satyakrishna Pentakota, John R. Weir, Yu-Chih Lin, Stefan Raunser, Andrew D. McAinsh, Andrea Musacchio Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80 published pages: 923-939.e10, ISSN: 1097-2765, DOI: 10.1016/j.molcel.2018.07.038 |
Molecular Cell 71/6 | 2019-10-08 |
| 2017 |
Katharina Overlack, Tanja Bange, Florian Weissmann, Alex C. Faesen, Stefano Maffini, Ivana Primorac, Franziska Müller, Jan-Michael Peters, Andrea Musacchio BubR1 Promotes Bub3-Dependent APC/C Inhibition during Spindle Assembly Checkpoint Signaling published pages: 2915-2927.e7, ISSN: 0960-9822, DOI: 10.1016/j.cub.2017.08.033 |
Current Biology 27/19 | 2019-10-08 |
| 2018 |
Vladimir A Volkov, Pim J Huis in \'t Veld, Marileen Dogterom, Andrea Musacchio Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.36764 |
eLife 7 | 2019-10-08 |
| 2015 |
Andrea Musacchio The Molecular Biology of Spindle Assembly Checkpoint Signaling Dynamics published pages: R1002-R1018, ISSN: 0960-9822, DOI: 10.1016/j.cub.2015.08.051 |
Current Biology 25/20 | 2019-07-04 |
| 2016 |
Yahui Liu, Arsen Petrovic, Pascaline Rombaut, Shyamal Mosalaganti, Jenny Keller, Stefan Raunser, Franz Herzog, Andrea Musacchio Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster published pages: 150236, ISSN: 2046-2441, DOI: 10.1098/rsob.150236 |
Open Biology 6/2 | 2019-07-04 |
| 2016 |
Arsen Petrovic, Jenny Keller, Yahui Liu, Katharina Overlack, Juliane John, Yoana N. Dimitrova, Simon Jenni, Suzan van Gerwen, Patricia Stege, Sabine Wohlgemuth, Pascaline Rombaut, Franz Herzog, Stephen C. Harrison, Ingrid R. Vetter, Andrea Musacchio Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores published pages: 1028-1040.e15, ISSN: 0092-8674, DOI: 10.1016/j.cell.2016.10.005 |
Cell 167/4 | 2019-07-04 |
| 2017 |
Dongqing Pan, Kerstin Klare, Arsen Petrovic, Annika Take, Kai Walstein, Priyanka Singh, Arnaud Rondelet, Alexander W Bird, Andrea Musacchio CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.23352 |
eLife 6 | 2019-07-04 |
| 2017 |
Shyamal Mosalaganti, Jenny Keller, Anika Altenfeld, Michael Winzker, Pascaline Rombaut, Michael Saur, Arsen Petrovic, Annemarie Wehenkel, Sabine Wohlgemuth, Franziska Müller, Stefano Maffini, Tanja Bange, Franz Herzog, Herbert Waldmann, Stefan Raunser, Andrea Musacchio Structure of the RZZ complex and molecular basis of its interaction with Spindly published pages: 961-981, ISSN: 0021-9525, DOI: 10.1083/jcb.201611060 |
The Journal of Cell Biology 216/4 | 2019-07-04 |
| 2017 |
Alex C. Faesen, Maria Thanasoula, Stefano Maffini, Claudia Breit, Franziska Müller, Suzan van Gerwen, Tanja Bange, Andrea Musacchio Basis of catalytic assembly of the mitotic checkpoint complex published pages: 498-502, ISSN: 0028-0836, DOI: 10.1038/nature21384 |
Nature 542/7642 | 2019-07-04 |
| 2016 |
Pim J Huis in \'t Veld, Sadasivam Jeganathan, Arsen Petrovic, Priyanka Singh, Juliane John, Veronica Krenn, Florian Weissmann, Tanja Bange, Andrea Musacchio Molecular basis of outer kinetochore assembly on CENP-T published pages: , ISSN: 2050-084X, DOI: 10.7554/eLife.21007 |
eLife 5 | 2019-07-04 |
| 2015 |
Veronica Krenn, Andrea Musacchio The Aurora B Kinase in Chromosome Bi-Orientation and Spindle Checkpoint Signaling published pages: , ISSN: 2234-943X, DOI: 10.3389/fonc.2015.00225 |
Frontiers in Oncology 5 | 2019-07-04 |
| 2017 |
Andrea Musacchio, Arshad Desai A Molecular View of Kinetochore Assembly and Function published pages: 5, ISSN: 2079-7737, DOI: 10.3390/biology6010005 |
Biology 6/4 | 2019-07-04 |
| 2016 |
Marion E Pesenti, John R Weir, Andrea Musacchio Progress in the structural and functional characterization of kinetochores published pages: 152-163, ISSN: 0959-440X, DOI: 10.1016/j.sbi.2016.03.003 |
Current Opinion in Structural Biology 37 | 2019-07-04 |
| 2016 |
John R. Weir, Alex C. Faesen, Kerstin Klare, Arsen Petrovic, Federica Basilico, Josef Fischböck, Satyakrishna Pentakota, Jenny Keller, Marion E. Pesenti, Dongqing Pan, Doro Vogt, Sabine Wohlgemuth, Franz Herzog, Andrea Musacchio Insights from biochemical reconstitution into the architecture of human kinetochores published pages: 249-253, ISSN: 0028-0836, DOI: 10.1038/nature19333 |
Nature 537/7619 | 2019-07-04 |
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