ELONGAN SIGNED
Gene editing and in vitro approaches to understand conceptus elongation in ungulates
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0ELONGAN project word cloud
Explore the words cloud of the ELONGAN project. It provides you a very rough idea of what is the project "ELONGAN" about.
Project "ELONGAN" data sheet
The following table provides information about the project.
| Coordinator |
INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP
Organization address contact info |
| Coordinator Country | Spain [ES] |
| Total cost | 1˙480˙880 € |
| EC max contribution | 1˙480˙880 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2017-STG |
| Funding Scheme | ERC-STG |
| Starting year | 2017 |
| Duration (year-month-day) | from 2017-10-01 to 2022-09-30 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP | ES (MADRID) | coordinator | 1˙480˙880.00 |
Map
Project objective
In contrast to human or rodent embryos, ungulate embryos do not implant into the uterus right after blastocyst hatching. Before implantation, the hatched ungulate blastocyst must undergo dramatic morphological changes characterized by cell differentiation, proliferation and migration processes leading to the development of extra-embryonic membranes, the appearance of a flat embryonic disc and gastrulation. This prolonged preimplantation development is termed conceptus elongation and deficiencies on this process constitute the most frequent cause of reproductive failures in ungulates, including the 4 most relevant mammalian livestock species in Europe. The purpose of this project is to elucidate the factors involved in conceptus elongation by gene editing and in vitro culture approaches. A first objective will be to identify key genes involved in differentiation processes by RNA-seq analysis of different embryo derivatives from bovine conceptuses at different developmental stages. Subsequently, the function of some of the genes identified as well as others known to play a crucial role in mouse development or putatively involved in embryo-maternal interactions will be assessed. For this aim, bovine embryos in which a candidate gene has been ablated (KO) will be generated by CRISPR and transferred to recipient females to assess in vivo the function of such particular gene on conceptus development. A second set of experiments pursue the development of an in vitro system for conceptus elongation that would bypass the requirement for in vivo experiments. For this aim we will perform metabolomics and proteomics analyses of bovine uterine fluid at different stages and will use these data to rationally develop a culture system able to sustain conceptus development. The knowledge generated by this project will serve to develop strategies to enhance farming profitability by reducing embryonic loss and to understand Developmental Biology questions unanswered by the mouse model.
Deliverables
| Data Management Plan | Open Research Data Pilot | 2019-11-25 12:35:12 |
Take a look to the deliverables list in detail: detailed list of ELONGAN deliverables.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2018 |
Ismael Lamas-Toranzo, Eva Pericuesta, Pablo Bermejo-Ãlvarez Mitochondrial and metabolic adjustments during the final phase of follicular development prior to IVM of bovine oocytes published pages: 156-162, ISSN: 0093-691X, DOI: 10.1016/j.theriogenology.2018.07.007 |
Theriogenology 119 | 2019-05-22 |
| 2018 |
Ismael Lamas-Toranzo, Priscila Ramos-Ibeas, Eva Pericuesta, Pablo Bermejo-Ãlvarez Directions and applications of CRISPR technology in livestock research published pages: 292-300, ISSN: 1984-3143, DOI: 10.21451/1984-3143-ar2018-0075 |
Animal Reproduction 15/3 | 2019-05-22 |
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "ELONGAN" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "ELONGAN" are provided by the European Opendata Portal: CORDIS opendata.