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ELONGAN SIGNED

Gene editing and in vitro approaches to understand conceptus elongation in ungulates

Total Cost €

EC-Contrib. €

Partnership

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ELONGAN project word cloud

Explore the words cloud of the ELONGAN project. It provides you a very rough idea of what is the project "ELONGAN" about.

stages proliferation frequent editing requirement elucidate rodent experiments hatching sustain hatched reproductive vivo undergo right rationally uterus crispr differentiation transferred blastocyst morphological females ungulate serve unanswered candidate vitro purpose proteomics extra conceptuses model pursue failures gene function termed embryo derivatives perform migration play elongation implantation culture questions mammalian ablated conceptus human first interactions biology developmental mouse metabolomics ko profitability understand livestock farming constitute prolonged putatively embryos appearance membranes flat contrast seq fluid strategies data subsequently rna dramatic disc species embryonic preimplantation implant ungulates bovine cell gastrulation bypass genes deficiencies maternal reducing recipient uterine

Project "ELONGAN" data sheet

The following table provides information about the project.

Coordinator	INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP Organization address address: Carretera de la Coruna Km7.5 city: MADRID postcode: 28040 website: www.inia.es contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Spain [ES]
Total cost	1˙480˙880 €
EC max contribution	1˙480˙880 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2017-STG
Funding Scheme	ERC-STG
Starting year	2017
Duration (year-month-day)	from 2017-10-01 to 2022-09-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP INSTITUTO NACIONAL DE INVESTIGACION Y TECNOLOGIA AGRARIA Y ALIMENTARIA OA MP Organization address address: Carretera de la Coruna Km7.5 city: MADRID postcode: 28040 website: www.inia.es contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	ES (MADRID)	coordinator	1˙480˙880.00

Map

Project objective

In contrast to human or rodent embryos, ungulate embryos do not implant into the uterus right after blastocyst hatching. Before implantation, the hatched ungulate blastocyst must undergo dramatic morphological changes characterized by cell differentiation, proliferation and migration processes leading to the development of extra-embryonic membranes, the appearance of a flat embryonic disc and gastrulation. This prolonged preimplantation development is termed conceptus elongation and deficiencies on this process constitute the most frequent cause of reproductive failures in ungulates, including the 4 most relevant mammalian livestock species in Europe. The purpose of this project is to elucidate the factors involved in conceptus elongation by gene editing and in vitro culture approaches. A first objective will be to identify key genes involved in differentiation processes by RNA-seq analysis of different embryo derivatives from bovine conceptuses at different developmental stages. Subsequently, the function of some of the genes identified as well as others known to play a crucial role in mouse development or putatively involved in embryo-maternal interactions will be assessed. For this aim, bovine embryos in which a candidate gene has been ablated (KO) will be generated by CRISPR and transferred to recipient females to assess in vivo the function of such particular gene on conceptus development. A second set of experiments pursue the development of an in vitro system for conceptus elongation that would bypass the requirement for in vivo experiments. For this aim we will perform metabolomics and proteomics analyses of bovine uterine fluid at different stages and will use these data to rationally develop a culture system able to sustain conceptus development. The knowledge generated by this project will serve to develop strategies to enhance farming profitability by reducing embryonic loss and to understand Developmental Biology questions unanswered by the mouse model.

Deliverables

List of deliverables.
Data Management Plan	Open Research Data Pilot	2019-11-25 12:35:12

Take a look to the deliverables list in detail: detailed list of ELONGAN deliverables.

Publications

List of publications.
year	authors and title	journal	last update
2018	Ismael Lamas-Toranzo, Eva Pericuesta, Pablo Bermejo-Ãlvarez Mitochondrial and metabolic adjustments during the final phase of follicular development prior to IVM of bovine oocytes published pages: 156-162, ISSN: 0093-691X, DOI: 10.1016/j.theriogenology.2018.07.007	Theriogenology 119	2019-05-22
2018	Ismael Lamas-Toranzo, Priscila Ramos-Ibeas, Eva Pericuesta, Pablo Bermejo-Ãlvarez Directions and applications of CRISPR technology in livestock research published pages: 292-300, ISSN: 1984-3143, DOI: 10.21451/1984-3143-ar2018-0075	Animal Reproduction 15/3	2019-05-22

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The information about "ELONGAN" are provided by the European Opendata Portal: CORDIS opendata.