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DEMED SIGNED

Directed Evolution of Metalloenzymes through Electrochemical Droplet Microarrays

Total Cost €

EC-Contrib. €

Partnership

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DEMED project word cloud

Explore the words cloud of the DEMED project. It provides you a very rough idea of what is the project "DEMED" about.

synthesized cloned random fuel model serve biofuel co curie oxygen first microarray never gene practical screened microarrays cells direct interface biocathodes oxidoreductase reducing electron metalloenzymes enzyme o2 library h2 biocathode roo individual active electrochemical demed performances advantageous electrode cell rubredoxin performance polymers fabrication transfer redox enzymatic evolution groundbreaking droplet rates fellowship directed screening enzymes mutagenesis desired metalloenzyme marie

Project "DEMED" data sheet

The following table provides information about the project.

Coordinator	RUHR-UNIVERSITAET BOCHUM Organization address address: UNIVERSITAETSSTRASSE 150 city: BOCHUM postcode: 44801 website: www.rub.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Total cost	162˙806 €
EC max contribution	162˙806 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2019
Funding Scheme	MSCA-IF-EF-ST
Starting year	2020
Duration (year-month-day)	from 2020-12-01 to 2022-11-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	RUHR-UNIVERSITAET BOCHUM RUHR-UNIVERSITAET BOCHUM Organization address address: UNIVERSITAETSSTRASSE 150 city: BOCHUM postcode: 44801 website: www.rub.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (BOCHUM)	coordinator	162˙806.00

Map

Project objective

The goal of this Marie Curie Individual fellowship proposal is to establish directed evolution of redox enzymes by means of electrochemical microarrays (DEMED) to enable the direct screening of the enzyme properties desired for their application in electrochemical devices. An O2 reducing metalloenzyme for implementation in biocathodes of H2/O2 enzymatic fuel cells will serve as model system to demonstrate that directed evolution of such redox enzymes screened by electrochemical droplet microarray is advantageous to specifically improve biofuel cell performances. The selected metalloenzyme is rubredoxin: oxygen oxidoreductase (ROO), which has never been applied to H2/O2 enzymatic fuel cells so far. First, ROO gene will be cloned and its random mutagenesis library will be synthesized. Second, the electrochemical droplet microarray will be adapted to enable the screening of the desired properties of the metalloenzyme. Third, electrochemical directed evolution of ROO will be carried out. Finally, the interface of ROO and electrode based on redox active polymers will be co-evolved with ROO to achieve high electron transfer rates to the enzyme and thus enable the fabrication of a high performance biocathode. It is expected that this project will have a groundbreaking on directed evolution of metalloenzymes for their practical implementation in electrochemical devices.

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The information about "DEMED" are provided by the European Opendata Portal: CORDIS opendata.