PERFECTION SIGNED
Probing mechanisms of pathogen effector recognition by plant Resistance proteins to elevate defence gene activation
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0PERFECTION project word cloud
Explore the words cloud of the PERFECTION project. It provides you a very rough idea of what is the project "PERFECTION" about.
Project "PERFECTION" data sheet
The following table provides information about the project.
| Coordinator |
THE SAINSBURY LABORATORY
Organization address contact info |
| Coordinator Country | United Kingdom [UK] |
| Project website | http://www.tsl.ac.uk/staff/pingtao-ding/ |
| Total cost | 183˙454 € |
| EC max contribution | 183˙454 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2014 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2016 |
| Duration (year-month-day) | from 2016-04-01 to 2018-03-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | THE SAINSBURY LABORATORY | UK (NORWICH) | coordinator | 183˙454.00 |
Map
Project objective
Plant cells tightly regulate gene transcription in response to a changing environment. Stresses, such as pathogen encounters, lead to dramatic transcriptional reprogramming to favor defence activation over basal cellular functions. For effective defence, cells must rapidly alter defence gene mRNA abundance. How extracellular and intracellular recognition of plant pathogens trigger appropriate changes in host mRNA abundance is poorly understood. Upon recognition of pathogen effectors, resistance proteins activate plant defence by unknown mechanisms. My host lab recently reported that the Arabidopsis Resistance gene pair RPS4/RRS1-R elevates expression of certain defence genes, such as those required for salicylic acid biosynthesis, within four hours of detecting PopP2 effector in Arabidopsis. The main goal of this proposal is to understand how effector recognition by RPS4/RRS1-R leads to rapid defence gene induction. We will test the hypothesis that RPS4/RRS1-R proteins directly interact with gene loci that are activated during this process. I will use transgenic Arabidopsis that carry a single genome locus with independently epitope-tagged RPS4, RRS1 and other defence-implicated proteins to investigate: (1) changes in composition of the RPS4/RRS1-R protein complex upon effector recognition using mass spectrometry; (2) effector-induced changes in association of the RPS4/RRS1-R proteins with induced genes using chromatin immunoprecipitation; (3) changes in chromatin status at induced gene regions correlated with gene induction and activation of defence by RPS4/RRS1-R. From this project, I will rigorously test an important model, namely that plant immune receptor complexes directly mediate transcriptional reprogramming of defence genes through chromatin changes upon recognition of effectors. I bring highly complementary expertise in genetics and transcriptional regulation of basal defence from China to Canada and now to TSL that is essential for this project’s success.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2017 |
Pingtao Ding, Jonathan D.G. Jones Mis-placed Congeniality: When Pathogens Ask Their Plant Hosts for Another Drink published pages: 116-117, ISSN: 1534-5807, DOI: 10.1016/j.devcel.2017.01.003 |
Developmental Cell 40/2 | 2019-06-13 |
| 2017 |
Sung Un Huh, Volkan Cevik, Pingtao Ding, Zane Duxbury, Yan Ma, Laurence Tomlinson, Panagiotis F. Sarris, Jonathan D. G. Jones Protein-protein interactions in the RPS4/RRS1 immune receptor complex published pages: e1006376, ISSN: 1553-7374, DOI: 10.1371/journal.ppat.1006376 |
PLOS Pathogens 13/5 | 2019-06-13 |
Are you the coordinator (or a participant) of this project? Plaese send me more information about the "PERFECTION" project.
For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.
Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.
Thanks. And then put a link of this page into your project's website.
The information about "PERFECTION" are provided by the European Opendata Portal: CORDIS opendata.
More projects from the same programme (H2020-EU.1.3.2.)
eXcape3D (2019)
Functional dissection of X-linked regulatory DNA: unravelling the impact of genome topology on transcriptional regulation
Read MorePaSION (2018)
A longitudinal assessment of treatment experience, symptoms and potential associations with biomarkers in cancer patients undergoing immune checkpoint inhibitor therapy
Read More