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HspAdhesion

Role of cell membrane associated Hsp70 in cancer cell adhesion and metastasis

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 HspAdhesion project word cloud

Explore the words cloud of the HspAdhesion project. It provides you a very rough idea of what is the project "HspAdhesion" about.

negative    interdisciplinary    molecule    folding    cancer    anchoring    molecules    elucidate    exhibits    biophysics    function    recognition    aggregation    distant    cells    clinical    unfolded    prevent    nascent    outer    hsp    substrates    upregulated    survival    nanometer    found    mimicking    differential    transport    variety    membrane    arrangement    protection    patients    technique    normal    topography    cellular    harmful    extracellular    proteins    inducible    biochemistry    resolution    drastically    employ    context    progression    interaction    specialized    assist    adhesion    heat    host    tissue    conferring    copes    invasive    spread    localization    microscopy    cell    chances    force    stresses    atomic    forces    denatured    tissues    quantify    protein    tumor    decreased    frequently    additional    medical    family    matrix    implicated    interface    indicate    hsp70    tumors    stress    simultaneous    spectroscopy    surface    afm    positive    shock    question    single    imaging    mediator    significance    highlight    metastasis   

Project "HspAdhesion" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAT LINZ 

Organization address
address: ALTENBERGER STRASSE 69
city: LINZ
postcode: 4040
website: www.jku.at

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Austria [AT]
 Project website http://www.jku.at/biophysics/content/e54900/e275954
 Total cost 166˙156 €
 EC max contribution 166˙156 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2015
 Duration (year-month-day) from 2015-05-01   to  2017-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAT LINZ AT (LINZ) coordinator 166˙156.00

Map

 Project objective

We propose an interdisciplinary project at the interface of biophysics, biochemistry, and medical research. Our objective is to investigate the specific role of cell membrane associated heat shock protein 70 (Hsp70) in the context of invasive cancer. In particular we address the question how cell membrane localization of Hsp70 affects differential adhesion of cancer cells and the formation of metastasis. In general, heat shock proteins (HSP) assist in folding of nascent proteins, prevent protein aggregation, and assist transport of proteins. Upon a variety of stresses HSP production is rapidly upregulated. Hsp70 is the major stress-inducible member of the HSP70 family. In normal cells it copes with harmful unfolded and denatured protein conferring protection to the cell. Tumor cells frequently present Hsp70 on their outer surface where it exhibits additional activities. For a number of different tumors a high level of membrane Hsp70 has been found to indicate drastically decreased survival chances in patients. Moreover it has been implicated in formation of metastasis where it might support spread and anchoring into distant tissues. These findings highlight the clinical significance of cell membrane associated Hsp70 and the need for a better understanding of its role in differential cell adhesion and progression of cancer. We will employ atomic force microscopy (AFM) in order to elucidate the function of membrane Hsp70 as a possible adhesion molecule or mediator of cellular adhesion. (1) Using single-cell force spectroscopy we will characterize and quantify adhesion forces of Hsp70 positive and negative cells to different substrates mimicking their interaction with extracellular matrix and host tissue. (2) With the help of a specialized AFM technique that enables topography imaging with a simultaneous recognition of specific surface molecules we will determine the localization and arrangement of Hsp70 molecules on the cell surface with nanometer resolution.

 Publications

year authors and title journal last update
List of publications.
2018 Constanze Lamprecht, Mathias Gehrmann, Josef Madl, Winfried Römer, Gabriele Multhoff, Andreas Ebner
Molecular AFM imaging of Hsp70-1A association with dipalmitoyl phosphatidylserine reveals membrane blebbing in the presence of cholesterol
published pages: , ISSN: 1355-8145, DOI: 10.1007/s12192-018-0879-0
Cell Stress and Chaperones 2019-07-23
2017 Michael Leitner Alexandra Poturnayova Constanze Lamprecht Sabine Weich Maja SnejdarkovaIvana Karpisova Tibor Hianik Andreas Ebner
Characterization of the specific interaction between the DNA aptamer sgc8c and protein tyrosine kinase-7 receptors at the surface of T-cells by biosensing AFM
published pages: , ISSN: 1618-2642, DOI: 10.1007/s00216-017-0238-5
Analytical and Bioanalytical Chemistry 2019-07-23
2017 Constanze Lamprecht, Jürgen Strasser, Lilia A. Chtcheglova, Melanie Köhler, Sandra Posch, Yoo Jin Oh, Rong Zhu, Andreas Ebner, Peter Hinterdorfer
Biomedical Sensing with the Atomic Force Microscope
published pages: 135-173, ISSN: , DOI: 10.1007/978-3-319-51433-8_4
Nanotribology and Nanomechanics 2019-07-23

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