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Report

Teaser, summary, work performed and final results

Periodic Reporting for period 2 - RNA ORIGAMI (RNA-protein Nanostructures for Synthetic Biology)

Teaser

Summary

The RNA ORIGAMI project aims at rationally designing RNA nanostructures to serve as scaffolds for controlling e.g. protein function in cells. This technology can potentially be used for a wide range of applications in synthetic biology such as production of cheaper drugs, sustainable fuel production, efficient diagnosis, and targeted therapy. The main objectives of the RNA ORIGAMI project are to develop design software, to characterize designed RNA scaffolds, to develop RNA sensor devices, and to demonstrate proof-of-concept applications in synthetic biology.

Work performed

The RNA ORIGAMI project has initially developed software for the rapid design of RNA origami structures and adapted methods for easy construction of expression vectors. Biophysical characterization methods have been used to verify the folding of the designed structures and biochemical methods have been used to verify the expression and stability when expressed in cells. Main results so far have been the demonstration of protein scaffolding, development of fluorescent reporter systems, and demonstration of dynamic devices.

Final results

The RNA ORIGAMI project has made progress beyond state of the art by developing a sensitive reporter system based on fluorescent aptamers and by constructing novel RNA-protein nanostructures. During the remaining time of the project the RNA ORIGAMI team will develop the technology further, but now have a strong focus on demonstrating applications of the technology as a tool for both basic cell biology research and synthetic biology applications.