Explore the words cloud of the CollBioImag project. It provides you a very rough idea of what is the project "CollBioImag" about.
The following table provides information about the project.
Coordinator |
THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE
Organization address contact info |
Coordinator Country | United Kingdom [UK] |
Project website | https://gbernardeslab.com/ |
Total cost | 195˙454 € |
EC max contribution | 195˙454 € (100%) |
Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
Code Call | H2020-MSCA-IF-2015 |
Funding Scheme | MSCA-IF-EF-ST |
Starting year | 2016 |
Duration (year-month-day) | from 2016-03-01 to 2018-02-28 |
Take a look of project's partnership.
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1 | THE CHANCELLOR MASTERS AND SCHOLARSOF THE UNIVERSITY OF CAMBRIDGE | UK (CAMBRIDGE) | coordinator | 195˙454.00 |
Liver fibrosis (hepatitis B and C, cirrhosis, etc) represents an enormous health burden responsible for ~1.03 millions death per year worldwide according to the World Health Organization. Fibrosis is also a common outcome of many chronic diseases of the kidney (diabetic nephropathy), lungs (idiopathic pulmonary fibrosis), heart and vasculature (heart failure). In pathologies where fibrosis is a feature, there is an increased deposition of extracellular matrix proteins, including collagens that dramatically limit tissue function. Herein we propose to develop a cell-based assay for high-throughput screening of new antifibrotic therapeutics, which will accelerate fibrosis drug development. Our approach consists in the metabolic incorporation of an alkene-tagged proline in the biosynthesis of collagen, followed by labelling with a fluorescent tetrazine. We recently published preliminary results that provide proof of principle for the use of proline derivatives equipped with reactive handles to tag and label collagen structures. However, this approach suffered from nonspecific reactions of the fluorescent probe with intracellular proteins. The level of selectivity conferred by the proposed inverse-electron-demand Diels-Alder reaction is key in enabling its use in cells. We will use hepatic stellate cells since these cells are responsible for excess collagen production during liver fibrosis. To demonstrate that the cellular model can be used to screen compounds for fibrosis we will validate our system using a library of compounds with known preclinical antifibrotic activities. We will test if their in vivo efficacy can be predicted using cells. If our findings are significant we will use this cellular model to screen a commercial available library of compounds to find new antifibrotic drugs. The efficacy of the best candidate will be tested in vivo using two animal models of induced fibrosis.
year | authors and title | journal | last update |
---|---|---|---|
2018 |
Maria J. Matos, Bruno L. Oliveira, Nuria MartÃnez-Sáez, Ana Guerreiro, Pedro M. S. D. Cal, Jean Bertoldo, MarÃa Maneiro, Elizabeth Perkins, Julie Howard, Michael J. Deery, Justin M. Chalker, Francisco Corzana, Gonzalo Jiménez-Osés, Gonçalo J. L. Bernardes Chemo- and Regioselective Lysine Modification on Native Proteins published pages: 4004-4017, ISSN: 0002-7863, DOI: 10.1021/jacs.7b12874 |
Journal of the American Chemical Society 140/11 | 2019-06-13 |
2018 |
Benjamin Stenton, Bruno Oliveira, Maria Matos, Laura Sinatra, Gonçalo J. L. Bernardes A Thioether-directed Palladium-cleavable Linker for Targeted Bioorthogonal Drug Decaging published pages: , ISSN: 2041-6520, DOI: 10.1039/C8SC00256H |
Chemical Science | 2019-06-13 |
2017 |
Ester Jiménez-Moreno, Zijian Guo, Bruno L. Oliveira, Inês S. Albuquerque, Annabel Kitowski, Ana Guerreiro, Omar Boutureira, Tiago Rodrigues, Gonzalo Jiménez-Osés, Gonçalo J. L. Bernardes Vinyl Ether/Tetrazine Pair for the Traceless Release of Alcohols in Cells published pages: 243-247, ISSN: 1433-7851, DOI: 10.1002/anie.201609607 |
Angewandte Chemie International Edition 56/1 | 2019-06-13 |
2017 |
B. L. Oliveira, Z. Guo, G. J. L. Bernardes Inverse electron demand Diels–Alder reactions in chemical biology published pages: 4895-4950, ISSN: 0306-0012, DOI: 10.1039/c7cs00184c |
Chemical Society Reviews 46/16 | 2019-06-13 |
2016 |
Barbara Bernardim, Pedro M.S.D. Cal, Maria J. Matos, Bruno L. Oliveira, Nuria MartÃnez-Sáez, Inês S. Albuquerque, Elizabeth Perkins, Francisco Corzana, Antonio C.B. Burtoloso, Gonzalo Jiménez-Osés, Gonçalo J. L. Bernardes Stoichiometric and irreversible cysteine-selective protein modification using carbonylacrylic reagents published pages: 13128, ISSN: 2041-1723, DOI: 10.1038/ncomms13128 |
Nature Communications 7 | 2019-06-13 |
2016 |
Zijian Guo, Bruno L. Oliveira, Omar Boutureira, Ana Guerreiro, and Gonçalo J. L. Bernardes A minimal, unstrained S-allyl handle for protein site-selective modification. published pages: , ISSN: , DOI: |
EMBL conference 2016, Heidelberg, Germany. | 2019-06-13 |
2016 |
Oliveira BL, Guo Z, Boutureira O, Guerreiro A, Jiménez-Osés G, Bernardes GJ A Minimal, Unstrained S-Allyl Handle for Pre-Targeting Diels-Alder Bioorthogonal Labeling in Live Cells. published pages: 14683-14687, ISSN: 1521-3773, DOI: 10.1002/anie.201608438 |
Angew Chem Int Ed Engl. 55(47) | 2019-06-13 |
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The information about "COLLBIOIMAG" are provided by the European Opendata Portal: CORDIS opendata.
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