Opendata, web and dolomites

NMR-DisAgg SIGNED

The Dynamic Composition of the Protein Chaperone Network: Unraveling Human Protein Disaggregation via NMR Spectroscopy

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NMR-DisAgg project word cloud

Explore the words cloud of the NMR-DisAgg project. It provides you a very rough idea of what is the project "NMR-DisAgg" about.

protein    diseases    labeling    nature    recognition    proven    complexes    functions    combining    structural    cest    biophysical    families    combinations    apart    dissolving    additional    human    diverse    operate    break    functional    recognizing    shsp    toxic    chaperones    group    neurodegenerative    hsp70    members    interactions    linked    course    shock    fibers    maintaining    perform    experiments    responsible    reaction    exact    though    observe    performed    performing    certain    client    little    methyl    clients    amyloids    time    initial    ideally    suited    structure    small    itself    protect    techniques    critical    transient    chaperone    discovered    substrate    disaggregation    regarding    amyloid    dnaj    refolding    schemes    potentially    cpmg    disease    series    homeostasis    populated    assays    host    lab    monitor    cellular    aside    ultimately    trosy    remodeling    characterization    first    molecular    types    hsp40    extremely    nmr    aggregates    cells    heat    proteins    dynamic   

Project "NMR-DisAgg" data sheet

The following table provides information about the project.

Coordinator
WEIZMANN INSTITUTE OF SCIENCE 

Organization address
address: HERZL STREET 234
city: REHOVOT
postcode: 7610001
website: www.weizmann.ac.il

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Israel [IL]
 Total cost 1˙499˙956 €
 EC max contribution 1˙499˙956 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2018-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-09-01   to  2023-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    WEIZMANN INSTITUTE OF SCIENCE IL (REHOVOT) coordinator 1˙499˙956.00

Map

 Project objective

Molecular chaperones are a diverse group of proteins critical to maintaining cellular homeostasis. Aside from protein refolding, it has recently been discovered that certain combinations of human chaperones can break apart toxic protein aggregates and even amyloids that have been linked to a host of neurodegenerative diseases. The first chaperones in this disaggregation reaction that are responsible for recognizing and performing initial remodeling of aggregates, are members of the Hsp40 (DnaJ) and small heat shock protein (sHSP) families. Very little, though, is known regarding how these chaperones perform their functions, and characterization of sHsp- and DnaJ-substrate complexes by most structural techniques has proven extremely challenging, as most chaperones are dynamic in nature and typically operate through a series of transient interactions with both their clients and other chaperones. The advanced NMR techniques used in our lab, however, are ideally suited for the study of these exact types of dynamic systems, and include recently developed experiments (CEST, CPMG) that allow us to monitor the transient and low populated protein states typical of chaperone-chaperone and chaperone-client interactions, as well as to study the structure of these potentially very large protein complexes (methyl-TROSY). By exploiting these NMR methodologies and additional, novel labeling schemes, we will characterize, for the first time, the recognition and substrate remodeling performed by the many members of the DnaJ and sHsp chaperone families on their clients. We will then take these approaches one step further and develop real time NMR experiments to observe the client remodeling performed over the course of the disaggregation reaction itself. By combining advanced NMR with biophysical and functional assays, we ultimately aim to identify the specific sets of chaperones that, with the Hsp70 system, protect our cells by dissolving disease-linked aggregates and amyloid fibers.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NMR-DISAGG" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NMR-DISAGG" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

RECON (2019)

Reprogramming Conformation by Fluorination: Exploring New Areas of Chemical Space

Read More  

ArtHistCEE (2018)

Art Historiographies in Central and Eastern EuropeAn Inquiry from the Perspective of Entangled Histories

Read More  

HD-Neu-Screen (2020)

HD-MEA-based Neuronal Assays and Network Analysis for Phenotypic Drug Screenings

Read More