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KMET-READ

Reading of lysine methylation – discovery, biological function and application

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

KMET-READ project word cloud

Explore the words cloud of the KMET-READ project. It provides you a very rough idea of what is the project "KMET-READ" about.

cds chromodomains discover biophysics initiate patented alter assays confocal fluorescence shown readers biological gene peptide mass reading proteins plans mammalian spectrometry induce act kmet effect skills solving methylation communication efficient excellent recognize career protein environment international methyltransferase histone phds human stability training modulates structure bind manifest fellow techniques seq cd influence cells suv39h1 read identification sustainable pulldowns residues spectroscopy circular methyltransferases chromatin maximize lysine interdisciplinary biology microscopy crystallography suv39h2 mll2 arrays expression anisotropy market domains marks phd chip plant collaborations mll3 mark yeast scaffold found chances hybrid binding homeodomains molecular biochemistry dichroism

Project "KMET-READ" data sheet

The following table provides information about the project.

Coordinator	UNIVERSITAET STUTTGART Organization address address: KEPLERSTRASSE 7 city: STUTTGART postcode: 70174 website: www.uni-stuttgart.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Project website	https://www.ibtb.uni-stuttgart.de/bc/forschung/eumc/index.html
Total cost	171˙460 €
EC max contribution	171˙460 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2014
Funding Scheme	MSCA-IF-EF-ST
Starting year	2015
Duration (year-month-day)	from 2015-10-01 to 2017-09-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	UNIVERSITAET STUTTGART UNIVERSITAET STUTTGART Organization address address: KEPLERSTRASSE 7 city: STUTTGART postcode: 70174 website: www.uni-stuttgart.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (STUTTGART)	coordinator	171˙460.00

Map

Project objective

Protein methylation at lysine residues modulates chromatin structure, affects gene expression and mammalian development. Recently, it was also shown to influence the stability and activity of non-histone proteins. Lysine methylation marks manifest their biological effect via so-called ‘readers’ (or reading domains) which recognize and bind the methylation mark and directly alter the chromatin structure or act as a scaffold for other proteins, which induce biological responses. Reading domains include Plant homeodomains (PHD) and Chromodomains (CD) found in many chromatin proteins. KMET-READ plans to investigate the biological role of these reading domains in essential histone lysine methyltransferases - PHDs in MLL2 and MLL3 and CDs in SUV39H1 and SUV39H2. The results obtained here will advance the understanding of chromatin changes in human cells. The proposal will apply an interdisciplinary approach in an international environment to maximize its chances of success: the biological role of reading domains will be evaluated with molecular biology (histone and chromatin pulldowns, ChIP-seq, confocal microscopy), biochemistry (Peptide arrays, mass spectrometry, methyltransferase activity assays) and biophysics techniques (fluorescence anisotropy, circular dichroism spectroscopy) as well as crystallography (solving the structure of reading domains). Importantly, the KMET-READ project will also develop a yeast-3-hybrid method for the identification of new reading domains, which will allow to discover binding partners for just recently characterized new protein methylation marks. This novel method will be patented and introduced into market. All project partners will ensure an efficient dissemination and communication of the results of the KMET-READ project. The project will provide excellent training in research methods and other skills for the fellow that will strongly support her future career and initiate new and sustainable collaborations between the partners.

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The information about "KMET-READ" are provided by the European Opendata Portal: CORDIS opendata.