#	Pagina
attuale pagina	/open-h2020/projects/194979/index.html

Opendata, web and dolomites

EVOPRIMPOL

Artificial evolution of a novel multifunctional human polymerase

Total Cost €

EC-Contrib. €

Partnership

Views

Outcomes and
results

EVOPRIMPOL project word cloud

Explore the words cloud of the EVOPRIMPOL project. It provides you a very rough idea of what is the project "EVOPRIMPOL" about.

superfamilies primases spain protocols performed randomised mutagenesis mimicking evoprimpol polymerases group variety fast members luis variants homology human professor vitro sequence biochemical uses site polymerase gene characterization superfamily chosen standard absence tolerance selecting cancer dna function evolution primpols containing shown enzyme accomplished crystal discovery biotechnological genes regulation critical novelty maintenance replication structure directed benefits rational madrid deeper residues damage select natural generate blanco primpol displays cells acids rounds subjected catalytic scheme priming performing diseases gives phylogenetic integrity solved ccdc111 protein amino malfunction therapy iterative unlike cbmso mutated

Project "EVOPRIMPOL" data sheet

The following table provides information about the project.

Coordinator	AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS Organization address address: CALLE SERRANO 117 city: MADRID postcode: 28006 website: http://www.csic.es contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Spain [ES]
Total cost	170˙121 €
EC max contribution	170˙121 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2014
Funding Scheme	MSCA-IF-EF-ST
Starting year	2015
Duration (year-month-day)	from 2015-05-01 to 2017-04-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS Organization address address: CALLE SERRANO 117 city: MADRID postcode: 28006 website: http://www.csic.es contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	ES (MADRID)	coordinator	170˙121.00

Map

Project objective

The product of the human gene ccdc111, PrimPol, has been recently identified and characterized by the group of Professor Luis Blanco (CBMSO, Madrid, Spain). This protein is capable of performing both priming and polymerase activities in human cells, contributing to DNA integrity and maintenance during its replication. As an enzyme involved in tolerance to DNA damage, its malfunction gives rise to human diseases. Thus, it might become a target for cancer therapy. In order to exploit the benefits derived from knowledge of PrimPol, a deeper structure-function study must be done. These studies can not be accomplished using standard site-directed mutagenesis protocols and further characterization due to variety activities that this protein displays, the novelty of its discovery and the absence of a solved crystal structure. Thus, EVOPRIMPOL will be a novel, fast and reliable approach to study the role of critical amino acids involved in the different activities shown by the enzyme. This approach is based on the in vitro directed evolution of human gene ccdc111, but unlike other directed studies performed in polymerases, where genes are mutated randomly, in EVOPRIMPOL we will use a new rational design to select those residues that will be randomised; selecting them after the phylogenetic study of all members of the protein superfamily containing PrimPols as well as by homology sequence studies using different DNA polymerases and primases from other superfamilies. Chosen residues will be randomised following an iterative mutagenesis scheme, where evolved variants are subjected to new rounds of mutagenesis mimicking the natural evolution. Thus, improved PrimPols will be obtained with better catalytic properties. Biochemical study of these variants will provide us information about the role of critical residues in the control and regulation of the PrimPol activities, which might be used to generate PrimPols variants with potential biotechnological uses.

Publications

List of publications.
year	authors and title	journal	last update
2017	RubÃ©n Agudo Patricia A. Calvo MarÃa I. MartÃnez-JimÃ©nez Luis Blanco Engineering human PrimPol into an efficient RNA-dependent-DNA primase/polymerase published pages: , ISSN: 0305-1048, DOI:	Nucleic Acid Research	2019-07-23

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "EVOPRIMPOL" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "EVOPRIMPOL" are provided by the European Opendata Portal: CORDIS opendata.