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EM-Pillars SIGNED

Controlled cryo-EM sample preparation through DNA-Origami pillars

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

EM-Pillars project word cloud

Explore the words cloud of the EM-Pillars project. It provides you a very rough idea of what is the project "EM-Pillars" about.

preparation despite tools correction thereby dna ice electron interface microscope made obtain hydrophobic particles prevent complexes optimal unfolding beam objects motion cryomicroscopy structural freezing origami small preferential reproducibility accuracy air outstanding protein nano cryo orientation limited variety powerful added resolution weaknesses strategies technique grid regarding ample structures structure room water relatively em improvement proteins thickness

Project "EM-Pillars" data sheet

The following table provides information about the project.

Coordinator	MEDICAL RESEARCH COUNCIL Organization address address: NORTH STAR AVENUE POLARIS HOUSE 2 FLOOR DAVID PHILLIPS BUILDING city: SWINDON postcode: SN2 1FL website: www.mrc.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	United Kingdom [UK]
Project website	http://www2.mrc-lmb.cam.ac.uk/
Total cost	183˙454 €
EC max contribution	183˙454 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2014
Funding Scheme	MSCA-IF-EF-ST
Starting year	2015
Duration (year-month-day)	from 2015-08-01 to 2017-07-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	MEDICAL RESEARCH COUNCIL MEDICAL RESEARCH COUNCIL Organization address address: NORTH STAR AVENUE POLARIS HOUSE 2 FLOOR DAVID PHILLIPS BUILDING city: SWINDON postcode: SN2 1FL website: www.mrc.ac.uk contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	UK (SWINDON)	coordinator	183˙454.00

Map

Project objective

Electron cryomicroscopy (cryo-EM) is rapidly emerging as a powerful technique for high-resolution structure determination of protein complexes. Despite recent advances, there is still ample room for improvement of this method, in particular regarding sample preparation. Outstanding challenges are reproducibility of freezing conditions to obtain an optimal ice thickness; to prevent unfolding or preferential orientation of proteins against the hydrophobic air-water interface; and a limited accuracy of beam-induced motion correction for relatively small particles. Here, we propose to study how DNA origami objects that are added in with the sample may affect cryo-EM grid preparation. In addition, by examining a variety of DNA origami structures in the electron microscope, we also aim to identify and improve on structural weaknesses in their design. We will test new design strategies, analyse those, and use them for application in cryo-EM. Thereby, this proposal will facilitate both cryo-EM structure determination and the design of nano-tools that are made from DNA.

Publications

List of publications.
year	authors and title	journal	last update
2016	Thomas G. Martin, Tanmay A. M. Bharat, Andreas C. Joerger, Xiao-chen Bai, Florian Praetorius, Alan R. Fersht, Hendrik Dietz, Sjors H. W. Scheres Design of a molecular support for cryo-EM structure determination published pages: E7456-E7463, ISSN: 0027-8424, DOI: 10.1073/pnas.1612720113	Proceedings of the National Academy of Sciences 113/47	2019-06-14
2016	Monika Bokori-Brown, Thomas G. Martin, Claire E. Naylor, Ajit K. Basak, Richard W. Titball, Christos G. Savva Cryo-EM structure of lysenin pore elucidates membrane insertion by an aerolysin family protein published pages: 11293, ISSN: 2041-1723, DOI: 10.1038/ncomms11293	Nature Communications 7	2019-06-14

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