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qCHROMDEK SIGNED

Quantitative insight into chromatin nanoscale structure: sub-nuclear organisation of oncoprotein DEK

Total Cost €

EC-Contrib. €

Partnership

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qCHROMDEK project word cloud

Explore the words cloud of the qCHROMDEK project. It provides you a very rough idea of what is the project "qCHROMDEK" about.

precise expression connection input structure levels edge consistently differs dimentional connected gain molecule origami dimensions proteins nucleosome tumour discrete cellular relation stochastic localisation deciphered mimic recognition nucleosomes experimental single imaging advantage biophysical protein bi rely cells normal correlation progression super additional biology clutch dek playing groups microscopy subnuclear stage malignancy reconstruction cell form clutches storm comprehension dna structures revealed oncoprotein optical organisation local calibrate possibility resolution tumorigenesis quantitative marker proliferation cancer cutting hypothesize densities construction tool chromatin architectural

Project "qCHROMDEK" data sheet

The following table provides information about the project.

Coordinator	FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA Organization address address: VIA MOREGO 30 city: GENOVA postcode: 16163 website: www.iit.it contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Italy [IT]
Total cost	171˙473 €
EC max contribution	171˙473 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2018
Funding Scheme	MSCA-IF-EF-ST
Starting year	2019
Duration (year-month-day)	from 2019-12-01 to 2021-11-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA Organization address address: VIA MOREGO 30 city: GENOVA postcode: 16163 website: www.iit.it contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	IT (GENOVA)	coordinator	171˙473.00

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Project objective

'DEK protein is a chromatin architectural factor which has been consistently associated with tumour progression. Its expression level differs between normal and cancer cells, raising the possibility of using DEK as a tumour marker. On the other hand, super-resolution imaging revealed recently that nucleosomes can form discrete groups called 'clutches' of various dimensions and densities. However, the quantitative analysis of the connection between proteins playing chromatin architectural role and nucleosome clutches has not been yet deciphered. Within this project, I hypothesize that there is a correlation between the organisation of DEK oncoprotein and the local chromatin structure related to the proliferation level of cells. The overall aim of the proposed work is to provide a more precise, quantitative understanding of possible connection between DEK expression level and local chromatin structures and by that to gain the additional information about DEK role in cancer. This project will take advantage of cutting-edge biophysical tool such as DNA origami, single-molecule localisation microscopy and cellular biology. Experimental design is based on the construction and application of a bi-dimentional DNA origami to calibrate and mimic subnuclear structures. The quantitative approach will rely on STochastic Optical Reconstruction Microscopy (STORM). This study will be done using normal cells with different level of proliferation and cancer cells with different level of malignancy. Finding the relation between DEK expression, connected to the proliferation level of normal and cancer cells, and the number of nucleosomes per clutch could improve the comprehension of chromatin organisation role in different levels of tumorigenesis. Quantitative insight to the number of nucleosomes per clutch in relation to the level of cell proliferation could be in the future an input for a precise recognition of cancer in the early stage of tumorigenesis. '

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The information about "QCHROMDEK" are provided by the European Opendata Portal: CORDIS opendata.