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DECOR SIGNED

Dynamic assembly and exchange of RNA polymerase II CTD factors

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

DECOR project word cloud

Explore the words cloud of the DECOR project. It provides you a very rough idea of what is the project "DECOR" about.

domain protein respect interactions talk molecular cycle multiple proline transiently modifications cross association coordinates resolution elucidate timing isomerisation questions isomer bases supramolecular diverse structures rna remodelled modulated transcriptional co stimulate transient assemblies bound translational nmr remained saxs conformational prevent dynamic post structure ctd assemble copies subunit basis polymerase mechanistic unravel compete poorly assembly associate patterns dictated answer exchange complexity rnapii composition flexible structural accessory terminal decorated transcription binding methodology combine decode modification

Project "DECOR" data sheet

The following table provides information about the project.

Coordinator	Masarykova univerzita Organization address address: Zerotinovo namesti 9 city: BRNO STRED postcode: 60177 website: http://www.muni.cz contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Czech Republic [CZ]
Project website	https://www.ceitec.eu/decor-dynamic-assembly-and-exchange-of-rna-polymerase-ii-ctd-factors/t2960
Total cost	1˙844˙603 €
EC max contribution	1˙844˙603 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2014-CoG
Funding Scheme	ERC-COG
Starting year	2015
Duration (year-month-day)	from 2015-08-01 to 2020-07-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	Masarykova univerzita Masarykova univerzita Organization address address: Zerotinovo namesti 9 city: BRNO STRED postcode: 60177 website: http://www.muni.cz contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	CZ (BRNO STRED)	coordinator	1˙844˙603.00

Map

Project objective

The C-terminal domain (CTD) of the RNA polymerase II (RNAPII) largest subunit coordinates co-transcriptional processing and it is decorated by many processing factors throughout the transcription cycle. The composition of this supramolecular assembly is diverse and highly dynamic. Many of the factors associate with RNAPII weakly and transiently, and the association is dictated by different post-translational modification patterns and conformational changes of the CTD. To determine how these accessory factors assemble and exchange on the CTD of RNAPII has remained a major challenge. Here, we aim to unravel the structural and mechanistic bases for the dynamic assembly of RNAPII CTD with its processing factors. Using NMR, we will determine high-resolution structures of several protein factors bound to the CTD carrying specific modifications. This will enable to decode how CTD modification patterns stimulate or prevent binding of a given processing factor. We will also establish the structural and mechanistic bases of proline isomerisation in the CTD that control the timing of isomer-specific protein-protein interactions. Next, we will combine NMR and SAXS approaches to unravel how the overall CTD structure is remodelled by binding of multiple copies of processing factors and how these factors cross-talk with each other. Finally, we will elucidate a mechanistic basis for the exchange of processing factors on the CTD. Our study will answer the long-standing questions of how the overall CTD structure is modulated on binding to processing factors, and whether these factors cross-talk and compete with each other. The level of detail that we aim to achieve is currently not available for any transient molecular assemblies of such complexity. In this respect, the project will also provide knowledge and methodology for further studies of large and highly flexible molecular assemblies that still remain poorly understood.

Publications

List of publications.
year	authors and title	journal	last update
2020	Zhong Han, Olga Jasnovidova, Nouhou Haidara, Agnieszka Tudek, Karel Kubicek, Domenico Libri, Richard Stefl, Odil Porrua Termination of nonâ€coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTDâ€mimicking region in Sen1 published pages: , ISSN: 0261-4189, DOI: 10.15252/embj.2019101548	The EMBO Journal 39/7	2020-04-15
2019	Pavel BrÃ¡zda, Ondrej Å edo, Karel KubÃÄek, Richard Å tefl Efficient and robust preparation of tyrosine phosphorylated intrinsically disordered proteins published pages: 16-22, ISSN: 0736-6205, DOI: 10.2144/btn-2019-0033	BioTechniques 67/1	2020-04-01
2017	Olga Jasnovidova, Tomas Klumpler, Karel Kubicek, Sergei Kalynych, Pavel Plevka, Richard Stefl Structure and dynamics of the RNAPII CTDsome with Rtt103 published pages: 11133-11138, ISSN: 0027-8424, DOI: 10.1073/pnas.1712450114	Proceedings of the National Academy of Sciences 114/42	2019-06-07
2017	Olga Jasnovidova, Magdalena Krejcikova, Karel Kubicek, Richard Stefl Structural insight into recognition of phosphorylated threonineâ€4 of RNA polymerase II Câ€terminal domain by Rtt103p published pages: 906-913, ISSN: 1469-221X, DOI: 10.15252/embr.201643723	EMBO reports 18/6	2019-06-07

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