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Rep. Fork Restart

Restart of blocked replication fork at replication fork barrier

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Rep. Fork Restart project word cloud

Explore the words cloud of the Rep. Fork Restart project. It provides you a very rough idea of what is the project "Rep. Fork Restart" about.

recombination    obstacles    bacteria    bypass    techniques    acquired    actors    gross    questions    collapsed    constantly    hybrids    fission    barriers    site    chromosomal    microscopy    muscular    independent    eukaryotic    bound    gain    2d    mechanism    errors    mutation    fails    electron    homologous    complete    characterised    me    replication    career    rts1    forming    sequences    characterise    converging    host    serious    rfb    nature    forks    understand    newly    rf    cells    rearrangements    challenged    recruitment    diseases    rfs    slippage    rna    prokaryotes    single    lesions    structures    restart    investigator    restarted    fellowship    neurological    inherited    data    stalled    chip    copy    hr    laboratory    unravel    formed    myself    transit    experts    fork    cancer    doctoral    gels    yeast    contacts    blocked    successfully    variations    training    proteins    error    lab    dna    prone    rfbs    mentoring    structure    molecular    rescued   

Project "Rep. Fork Restart" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF SUSSEX 

Organization address
address: SUSSEX HOUSE FALMER
city: BRIGHTON
postcode: BN1 9RH
website: http://www.sussex.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.address.com
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-05-01   to  2019-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF SUSSEX UK (BRIGHTON) coordinator 183˙454.00

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 Project objective

Errors in replication of DNA lead to serious diseases like cancer, inherited neurological and muscular diseases. Replication is constantly challenged by various obstacles that can result in error-prone replication. Replication fork barriers (RFBs) include proteins bound to DNA, structure forming sequences and DNA/RNA hybrids. Replication forks (RFs) stalled or blocked at these barriers can be rescued by a converging fork. But if this fails blocked RF must be restarted to complete replication. Restart using homologous recombination (HR) can lead to gross chromosomal rearrangements and copy number variations. The newly restarted replication fork is also prone to slippage leading to mutation and rearrangements. The research questions I will address are how this RF is restarted and why it is error prone. I will focus on restart at a site-specific RFB in fission yeast, RTS1. Restart at RTS1 has been well characterised in the host laboratory. My project will identify DNA structures formed during the RF restart and characterise recruitment of factors involved in the restart of the collapsed RF. The acquired data will enable us to further understand the mechanism of RF restart and to unravel the cause of the error prone nature of newly restarted RF. I consider this fellowship a key step in my career as it allows me to transit from the analysis of site-specific lesions in prokaryotes (previous doctoral fellowship) to eukaryotic cells, gain experience in a wide range of techniques (2D gels, Electron microscopy, ChIP) and build contacts with experts in the field of eukaryotic replication restart. This is necessary for my future independent research and will enable me to set up my lab to study molecular actors and DNA structures during bypass of single lesions in bacteria and eukaryotic cells. The mentoring during the fellowship will provide me with the necessary training to successfully establish myself as an independent investigator.

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The information about "REP. FORK RESTART" are provided by the European Opendata Portal: CORDIS opendata.

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