Opendata, web and dolomites

Rep. Fork Restart

Restart of blocked replication fork at replication fork barrier

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 Rep. Fork Restart project word cloud

Explore the words cloud of the Rep. Fork Restart project. It provides you a very rough idea of what is the project "Rep. Fork Restart" about.

diseases    contacts    nature    rescued    inherited    slippage    doctoral    mechanism    challenged    acquired    fails    forks    replication    newly    variations    single    bound    characterise    understand    data    rts1    rfs    converging    dna    eukaryotic    recombination    obstacles    gross    proteins    rfbs    copy    homologous    site    electron    host    microscopy    restart    complete    muscular    prokaryotes    hybrids    characterised    rna    constantly    investigator    lab    myself    blocked    bacteria    fork    questions    stalled    errors    sequences    mentoring    career    mutation    neurological    hr    fellowship    barriers    serious    prone    laboratory    unravel    forming    chip    2d    structure    me    rearrangements    actors    successfully    experts    lesions    training    techniques    collapsed    fission    transit    structures    yeast    gels    formed    recruitment    restarted    molecular    cancer    cells    chromosomal    independent    error    bypass    rfb    rf    gain   

Project "Rep. Fork Restart" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF SUSSEX 

Organization address
address: SUSSEX HOUSE FALMER
city: BRIGHTON
postcode: BN1 9RH
website: http://www.sussex.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://www.address.com
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-05-01   to  2019-04-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF SUSSEX UK (BRIGHTON) coordinator 183˙454.00

Map

 Project objective

Errors in replication of DNA lead to serious diseases like cancer, inherited neurological and muscular diseases. Replication is constantly challenged by various obstacles that can result in error-prone replication. Replication fork barriers (RFBs) include proteins bound to DNA, structure forming sequences and DNA/RNA hybrids. Replication forks (RFs) stalled or blocked at these barriers can be rescued by a converging fork. But if this fails blocked RF must be restarted to complete replication. Restart using homologous recombination (HR) can lead to gross chromosomal rearrangements and copy number variations. The newly restarted replication fork is also prone to slippage leading to mutation and rearrangements. The research questions I will address are how this RF is restarted and why it is error prone. I will focus on restart at a site-specific RFB in fission yeast, RTS1. Restart at RTS1 has been well characterised in the host laboratory. My project will identify DNA structures formed during the RF restart and characterise recruitment of factors involved in the restart of the collapsed RF. The acquired data will enable us to further understand the mechanism of RF restart and to unravel the cause of the error prone nature of newly restarted RF. I consider this fellowship a key step in my career as it allows me to transit from the analysis of site-specific lesions in prokaryotes (previous doctoral fellowship) to eukaryotic cells, gain experience in a wide range of techniques (2D gels, Electron microscopy, ChIP) and build contacts with experts in the field of eukaryotic replication restart. This is necessary for my future independent research and will enable me to set up my lab to study molecular actors and DNA structures during bypass of single lesions in bacteria and eukaryotic cells. The mentoring during the fellowship will provide me with the necessary training to successfully establish myself as an independent investigator.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "REP. FORK RESTART" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "REP. FORK RESTART" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

ACES (2019)

Antarctic Cyclones: Expression in Sea Ice

Read More  

OSeaIce (2019)

Two-way interactions between ocean heat transport and Arctic sea ice

Read More  

EcoSpy (2018)

Leveraging the potential of historical spy satellite photography for ecology and conservation

Read More