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OVO-GROWTH SIGNED

Oogenesis spotlighted: making mature human oocytes

Total Cost €

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EC-Contrib. €

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Partnership

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 OVO-GROWTH project word cloud

Explore the words cloud of the OVO-GROWTH project. It provides you a very rough idea of what is the project "OVO-GROWTH" about.

oocyte    cellular    mathematical    postnatal    perform    molecular    reveal    networks    fail    fertility    breakthrough    chemotherapy    hematopoietic    efficient    vivo    cancer    cells    graft    follicle    ovary    mini    gametogenesis    follicular    niche    trajectories    oocytes    cortical    netherlands    biopsies    successful    eggs    approval    ovarian    counterparts    humans    seq    outcomes    plan    drop    material    mice    strips    foetal    possibility    pregnancies    cryopreserve    conceive    too    childhood    patients    latest    model    oogenesis    damaged    single    transplantation    mechanisms    transcriptional    reproductive    benchmark    survive    maturation    personalized    women    expertise    led    preservation    patient    signalling    human    thousands    developmental    rare    profiling    xenotransplantation    adulthood    worldwide    assays    sequenced    explore    thereafter    life    systematically    age    toxicology    ethical    autologous    risk    later    mouse    tissue    hours    disease    somatic    identities    therapy    cell    reintroducing    alternatives    algorithms    engineer    vitro    profile    malignancies    organoid    gonadotoxic   

Project "OVO-GROWTH" data sheet

The following table provides information about the project.

Coordinator
ACADEMISCH ZIEKENHUIS LEIDEN 

Organization address
address: ALBINUSDREEF 2
city: LEIDEN
postcode: 2333 ZA
website: www.lumc.nl

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Netherlands [NL]
 Total cost 2˙000˙000 €
 EC max contribution 2˙000˙000 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-COG
 Funding Scheme ERC-COG
 Starting year 2017
 Duration (year-month-day) from 2017-07-01   to  2022-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    ACADEMISCH ZIEKENHUIS LEIDEN NL (LEIDEN) coordinator 2˙000˙000.00

Map

 Project objective

Women who survive childhood cancer often fail to conceive because their eggs are damaged by (gonadotoxic) chemotherapy. A major breakthrough has been the possibility to cryopreserve cortical strips of their ovarian tissue for autologous transplantation later in life. This has led to over 40 successful pregnancies worldwide, the latest in the Netherlands. However, the risk of reintroducing cancer cells with the ovarian graft in patients with previous hematopoietic malignancies is too great and alternatives are needed. Here, I propose to build on my expertise in gametogenesis in mice and humans and perform a detailed study of the cellular networks and molecular pathways that control development and maturation of the oocyte within the human ovary. We have access and ethical approval for research on human foetal tissue and postnatal ovarian biopsies over a wide age range. I will use this rare material to systematically benchmark the transcriptional profile of cells in the human ovary (oocytes as well as somatic cells) during development and adulthood using Drop-seq, a novel cost-efficient single-cell technology that allows the profiling of thousands of cells in a matter of hours. Thereafter, we will apply mathematical algorithms to reveal cellular identities, developmental trajectories and signalling networks that control oogenesis. With this knowledge, I plan to engineer a human follicular niche creating a “mini-ovary” in vitro that could support the formation and maturation of the oocyte (using patient-specific cells) and to explore mechanisms of follicle maturation through a xenotransplantation mouse model. The cellular outcomes of these assays will be sequenced using Drop-seq and directly compared to their in vivo counterparts. Our approach will lead to more effective personalized-therapy for fertility preservation and contribute to the development of an in vitro mini-ovary organoid model to use in human reproductive toxicology and disease modelling.

 Publications

year authors and title journal last update
List of publications.
2019 X. Fan, M. Bialecka, I. Moustakas, E. Lam, V. Torrens-Juaneda, N. V. Borggreven, L. Trouw, L. A. Louwe, G. S. K. Pilgram, H. Mei, L. van der Westerlaken, S. M. Chuva de Sousa Lopes
Single-cell reconstruction of follicular remodeling in the human adult ovary
published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-019-11036-9
Nature Communications 10/1 2020-01-29
2018 Ábel Vértesy, Wibowo Arindrarto, Matthias S. Roost, Björn Reinius, Vanessa Torrens-Juaneda, Monika Bialecka, Ioannis Moustakas, Yavuz Ariyurek, Ewart Kuijk, Hailiang Mei, Rickard Sandberg, Alexander van Oudenaarden, Susana M. Chuva de Sousa Lopes
Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells
published pages: na, ISSN: 2041-1723, DOI: 10.1038/s41467-018-04215-7
Nature Communications 9/1 2019-05-27
2017 Maria Gomes Fernandes, Nannan He, Fang Wang, Liesbeth Van Iperen, Cristina Eguizabal, Roberto Matorras, Bernard A J Roelen, Susana M Chuva De Sousa Lopes
Human-specific subcellular compartmentalization of P-element induced wimpy testis-like (PIWIL) granules during germ cell development and spermatogenesis
published pages: 258-269, ISSN: 0268-1161, DOI: 10.1093/humrep/dex365
Human Reproduction 33/2 2019-03-12
2018 Maria Gomes Fernandes, Monika Bialecka, Daniela C F Salvatori, Susana M Chuva de Sousa Lopes
Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesis
published pages: 233-243, ISSN: 1460-2407, DOI: 10.1093/molehr/gay011
MHR: Basic science of reproductive medicine 24/5 2019-03-12

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