Opendata, web and dolomites

OPTOLEADER SIGNED

Optogenetic control of leader cell mechanobiology during collective cell migration

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 OPTOLEADER project word cloud

Explore the words cloud of the OPTOLEADER project. It provides you a very rough idea of what is the project "OPTOLEADER" about.

epithelial    light    largely    biology    arising    integral    fundamental    expressing    leaders    transduced    combines    motility    emergent    blue    proteins    exhibiting    ecm    generate    behaviours    directional    tools    modifications    collective    shed    cohesive    play    stress    cell    confined    array    matrix    performed    contribution    intracellular    hallmark    invasion    optogenetics    activators    migration    influence    waves    physics    cells    create    paving    perform    guide    traction    reversible    wound    experimental    substrates    force    confluent    active    physiologically    situations    flocking    molecular    functionalized    prominently    groups    physical    physically    transduction    microscopy    sensitive    multicellular    forces    morphogenesis    actions    biological    quantification    flocks    leader    express    vivo    monolayer    migrating    migratory    orchestrated    dimensional    extracellular    monolayers    modulate    latter    healing    phenomena    cancer    adhesion    rhogtpases    mechanical   

Project "OPTOLEADER" data sheet

The following table provides information about the project.

Coordinator
FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA 

Organization address
address: CARRER BALDIRI REIXAC PLANTA 2A 10-12
city: BARCELONA
postcode: 8028
website: http://www.ibecbarcelona.eu

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Spain [ES]
 Total cost 158˙121 €
 EC max contribution 158˙121 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2017
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-09-01   to  2021-08-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA ES (BARCELONA) coordinator 158˙121.00

Map

 Project objective

Emergent collective behaviours such as flocks and waves are a hallmark of biological active matter, and occur prominently also in large groups of migrating epithelial cells, where they are involved in fundamental biological processes such as wound healing, morphogenesis and cancer cell invasion. Physical forces transduced between cells and arising between cells and the extracellular matrix (ECM) play an integral role in orchestrated multicellular phenomena. Another integral contribution is given by the actions of leader cells, that modulate and guide the migration of cohesive cell groups. How leader cells achieve this using intracellular and cell-ECM forces remains largely to be understood. Here we propose an experimental approach to generate leader cells using optogenetics and to study how leaders influence the collective behaviour of migratory cell groups. We will use epithelial cells expressing light-sensitive activators of RhoGTPases, which enable reversible and directional control of cell motility using blue light. Traction force microscopy and monolayer stress microscopy will be performed using these cells while we will create and control leaders. With functionalized substrates, we will study the mechanical role of leaders in different conditions, from confined two-cell systems to confluent monolayers exhibiting flocking. Finally, we will express the light-sensitive proteins in cancer cells, to study how their three-dimensional dissemination is affected by leaders. Our experimental approach combines physics-derived modelling and quantification of forces with advanced molecular biology tools. The latter will enable us to perform selected modifications on cells, targeting a wide array of proteins involved in cell-cell adhesion and force transduction. Our goal is to shed light on how leaders physically influence collective migration in physiologically relevant situations, paving the way for the in vivo applications of light-induced leader cells.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "OPTOLEADER" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "OPTOLEADER" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MarshFlux (2020)

The effect of future global climate and land-use change on greenhouse gas fluxes and microbial processes in salt marshes

Read More  

eXcape3D (2019)

Functional dissection of X-linked regulatory DNA: unravelling the impact of genome topology on transcriptional regulation

Read More  

VINCI (2020)

The Value of Information and Choice to Improve Control.

Read More