#	Pagina
attuale pagina	/open-h2020/projects/218266/index.html

Opendata, web and dolomites

5D-NanoTrack SIGNED

Five-Dimensional Localization Microscopy for Sub-Cellular Dynamics

Total Cost €

EC-Contrib. €

Partnership

Views

5D-NanoTrack project word cloud

Explore the words cloud of the 5D-NanoTrack project. It provides you a very rough idea of what is the project "5D-NanoTrack" about.

emitters life holy human efficacy base micro holds methodology engineering chromatin technique point tools dna spatiotemporal spatial volumetric lack offs spectral dynamics microscopy extremely pairs live speed track color variety imaging dense nanoscale overcome bottlenecks psfs sub signal powerful temporal dimensions fundamental suitable limited multicolor biological tracking optical organization diffractive 3d cellular trade cells positions ahead critical visualization capability revolutionized simultaneously observation 5d function spread unprecedented dynamic billion create multiple grail intrinsically uniquely plan psf pave cell time resolution inside super markers applicable appropriate divided observe inherent 3

Project "5D-NanoTrack" data sheet

The following table provides information about the project.

Coordinator	TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY Organization address address: SENATE BUILDING TECHNION CITY city: HAIFA postcode: 32000 website: www.technion.ac.il contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Israel [IL]
Total cost	1˙802˙500 €
EC max contribution	1˙802˙500 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2018-STG
Funding Scheme	ERC-STG
Starting year	2018
Duration (year-month-day)	from 2018-11-01 to 2023-10-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY Organization address address: SENATE BUILDING TECHNION CITY city: HAIFA postcode: 32000 website: www.technion.ac.il contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	IL (HAIFA)	coordinator	1˙802˙500.00

Map

Project objective

The sub-cellular processes that control the most critical aspects of life occur in three-dimensions (3D), and are intrinsically dynamic. While super-resolution microscopy has revolutionized cellular imaging in recent years, our current capability to observe the dynamics of life on the nanoscale is still extremely limited, due to inherent trade-offs between spatial, temporal and spectral resolution using existing approaches.

We propose to develop and demonstrate an optical microscopy methodology that would enable live sub-cellular observation in unprecedented detail. Making use of multicolor 3D point-spread-function (PSF) engineering, a technique I have recently developed, we will be able to simultaneously track multiple markers inside live cells, at high speed and in five-dimensions (3D, time, and color).

Multicolor 3D PSF engineering holds the potential of being a uniquely powerful method for 5D tracking. However, it is not yet applicable to live-cell imaging, due to significant bottlenecks in optical engineering and signal processing, which we plan to overcome in this project. Importantly, we will also demonstrate the efficacy of our method using a challenging biological application: real-time visualization of chromatin dynamics - the spatiotemporal organization of DNA. This is a highly suitable problem due to its fundamental importance, its role in a variety of cellular processes, and the lack of appropriate tools for studying it. The project is divided into 3 aims: 1. Technology development: diffractive-element design for multicolor 3D PSFs. 2. System design: volumetric tracking of dense emitters. 3. Live-cell measurements: chromatin dynamics.

Looking ahead, here we create the imaging tools that pave the way towards the holy grail of chromatin visualization: dynamic observation of the 3D positions of the ~3 billion DNA base-pairs in a live human cell. Beyond that, our results will be applicable to numerous 3D micro/nanoscale tracking applications.

Publications

List of publications.
year	authors and title	journal	last update
2019	Eran Hershko, Lucien E. Weiss, Tomer Michaeli, Yoav Shechtman Multicolor localization microscopy and point-spread-function engineering by deep learning published pages: 6158, ISSN: 1094-4087, DOI: 10.1364/oe.27.006158	Optics Express 27/5	2019-05-28

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "5D-NANOTRACK" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "5D-NANOTRACK" are provided by the European Opendata Portal: CORDIS opendata.