Opendata, web and dolomites

ACTOMYOSIN RING SIGNED

Understanding Cytokinetic Actomyosin Ring Assembly Through Genetic Code Expansion, Click Chemistry, DNA origami, and in vitro Reconstitution

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 ACTOMYOSIN RING project word cloud

Explore the words cloud of the ACTOMYOSIN RING project. It provides you a very rough idea of what is the project "ACTOMYOSIN RING" about.

decipher    dna    illuminate    eukaryotes    pertaining    self    hampered    generate    employing    cell    proteins    expressing    filaments    man    internal    total    conserved    myosins    modulators    actomyosin    organize    actively    yeast    cells    vi    fission    cytokinesis    permeabilized    visualize    fluorescent    fluorescence    force    arrangement    themselves    appropriate    native    bilayers    live    contractile    divides    assembled    expansion    actin    division    fundamental    principles    questions    pombe    mechanism    efforts    structural    assembly    genetics    reflection    machines    daughters    labelled    mother    lack    cytoskeletal    biochemistry    polarity    150    code    click    multimeric    schizosaccharomyces    generates    chemistry    reconstitute    origami    microscopy    spheroplasts    bisect    filamentous    functional    biological    imaging    synthetic    attempt    filament    debated    motors    cellular    myosin    biology    organization    dimeric    comprising    ring    genetic   

Project "ACTOMYOSIN RING" data sheet

The following table provides information about the project.

Coordinator
THE UNIVERSITY OF WARWICK 

Organization address
address: Kirby Corner Road - University House
city: COVENTRY
postcode: CV4 8UW
website: www.warwick.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Project website http://mechanochemistry.org/Balasubramanian/
 Total cost 2˙863˙705 €
 EC max contribution 2˙863˙705 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2014-ADG
 Funding Scheme ERC-ADG
 Starting year 2015
 Duration (year-month-day) from 2015-11-01   to  2020-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE UNIVERSITY OF WARWICK UK (COVENTRY) coordinator 2˙863˙705.00

Map

 Project objective

The mechanism of cell division is conserved in many eukaryotes, from yeast to man. A contractile ring of filamentous actin and myosin II motors generates the force to bisect a mother cell into two daughters. The actomyosin ring is among the most complex cellular machines, comprising over 150 proteins. Understanding how these proteins organize themselves into a functional ring with appropriate contractile properties remains one of the great challenges in cell biology. Efforts to generate a comprehensive understanding of the mechanism of actomyosin ring assembly have been hampered by the lack of structural information on the arrangement of actin, myosin II, and actin modulators in the ring in its native state. Fundamental questions such as how actin filaments are assembled and organized into a ring remain actively debated. This project will investigate key issues pertaining to cytokinesis in the fission yeast Schizosaccharomyces pombe, which divides employing an actomyosin based contractile ring, using the methods of genetics, biochemistry, cellular imaging, DNA origami, genetic code expansion, and click chemistry. Specifically, we will (1) attempt to visualize actin filament assembly in live cells expressing fluorescent actin generated through synthetic biological approaches, including genetic code expansion and click chemistry (2) decipher actin filament polarity in the actomyosin ring using total internal reflection fluorescence microscopy of labelled dimeric and multimeric myosins V and VI generated through DNA origami approaches (3) address when, where, and how actin filaments for cytokinesis are assembled and organized into a ring and (4) reconstitute actin filament and functional actomyosin ring assembly in permeabilized spheroplasts and in supported bilayers. Success in the project will provide major insight into the mechanism of actomyosin ring assembly and illuminate principles behind cytoskeletal self-organization.

 Publications

year authors and title journal last update
List of publications.
2018 Tzer Chyn Lim, Tomoyuki Hatano, Anton Kamnev, Mohan K. Balasubramanian, Ting Gang Chew
Equatorial Assembly of the Cell-Division Actomyosin Ring in the Absence of Cytokinetic Spatial Cues
published pages: 955-962.e3, ISSN: 0960-9822, DOI: 10.1016/j.cub.2018.01.088
Current Biology 28/6 2019-07-05
2018 Tomoyuki Hatano, Salvatore Alioto, Emanuele Roscioli, Saravanan Palani, Scott T. Clarke, Anton Kamnev, Juan Ramon Hernandez-Fernaud, Lavanya Sivashanmugam, Bernardo Chapa-y-Lazo, Alexandra M. E. Jones, Robert C. Robinson, Karuna Sampath, Masanori Mishima, Andrew D. McAinsh, Bruce L. Goode, Mohan K. Balasubramanian
Rapid production of pure recombinant actin isoforms in Pichia pastoris
published pages: jcs213827, ISSN: 0021-9533, DOI: 10.1242/jcs.213827
Journal of Cell Science 131/8 2019-07-05
2018 Saravanan Palani, Ramanujam Srinivasan, Paola Zambon, Anton Kamnev, Pananghat Gayathri, Mohan K. Balasubramanian
Steric hindrance in the upper 50 kDa domain of the motor Myo2p leads to cytokinesis defects in fission yeast
published pages: jcs205625, ISSN: 0021-9533, DOI: 10.1242/jcs.205625
Journal of Cell Science 131/1 2019-07-05

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "ACTOMYOSIN RING" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "ACTOMYOSIN RING" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

MuFLOART (2018)

Microbiological fluorescence observatory for antibiotic resistance tracking

Read More  

ModGravTrial (2019)

Modified Gravity on Trial

Read More  

OptimHist (2020)

Optimization and historical contingency in living systems: a biophysical approach

Read More