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Cohesin loading TERMINATED

Elucidating the molecular mechanism of cohesin-loading

Total Cost €

EC-Contrib. €

Partnership

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Cohesin loading project word cloud

Explore the words cloud of the Cohesin loading project. It provides you a very rough idea of what is the project "Cohesin loading" about.

molecule dna molecular occurs genome loading atp first until positioned tweezers loaded oocytes reaction chromatid telophase understand complexes single vitro functions rcs purified conformational cohesin template vital sister chromatin effect fulfill laevis minimal experiments xenopus vivo replication scc4 loads characterizing additional monitor optical formed cohesion reconstitute mitosis g1 scc2 poorly mediates fret strictly mechanism mechanistic depends structure mechanisms regulated

Project "Cohesin loading" data sheet

The following table provides information about the project.

Coordinator	FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH Organization address address: CAMPUS-VIENNA-BIOCENTER 1 city: WIEN postcode: 1030 website: www.imp.ac.at contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Austria [AT]
Total cost	166˙156 €
EC max contribution	166˙156 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2015
Funding Scheme	MSCA-IF-EF-ST
Starting year	2017
Duration (year-month-day)	from 2017-04-01 to 2019-03-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH Organization address address: CAMPUS-VIENNA-BIOCENTER 1 city: WIEN postcode: 1030 website: www.imp.ac.at contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	AT (WIEN)	coordinator	166˙156.00

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Project objective

The cohesin-complex mediates sister chromatid cohesion from S-phase until mitosis and is involved in the formation of higher-order chromatin structure. To fulfill these vital functions, cohesin is loaded and positioned in the genome by mechanisms that are only poorly understood. In vitro, loading of cohesin on DNA only requires ATP and a loading-complex formed by Scc2-Scc4, while loading in vivo on chromatin is regulated by additional factors. For example, in Xenopus laevis oocytes, cohesin loading strictly depends on pre-replication complexes (pre-RCs), which are formed in telophase/G1. Mechanistic studies are required to understand how cohesin-loading occurs at the molecular level. I will first determine the mechanism by which Scc2-Scc4 loads cohesin on DNA. Using single-molecule FRET and optical tweezers, I will monitor the effect of Scc2-Scc4 on conformational changes of cohesin as it is loaded on a DNA template. After characterizing this minimal loading reaction, I will reconstitute cohesin-loading during telophase/G1 using a purified system. With these experiments I will address why and how loading of cohesin is regulated by the formation of pre-RCs.

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The information about "COHESIN LOADING" are provided by the European Opendata Portal: CORDIS opendata.