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SINCHAIS SIGNED

In situ analysis of single channel subunit composition in neurons: physiological implication in synaptic plasticity and behavior

Total Cost €

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EC-Contrib. €

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Partnership

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 Outcomes and results

 SINCHAIS project word cloud

Explore the words cloud of the SINCHAIS project. It provides you a very rough idea of what is the project "SINCHAIS" about.

stimulation    microscopy    trafficking    synaptic    genetic    subunits    unprecedented    subunit    nmda    activation    composition    regulation    molecules    plasticity    ampa    electrophysiological    presynaptic    mice    detection    implications    course    alteration    transforming    efficacy    labelling    electron    replica    excitatory    postsynaptic    diversity    technologies    synapses    tomography    property    optogenetic    calcium    animal    behavioural    sites    connections    channels    voltage    ones    fracture    little    biochemical    single    individual    manipulation    combining    gated    effectors    physiological    interaction    dynamic    combined    release    currents    tissues    brain    electrical    insights    situ    receptors    intact    examine    freeze    glutamate    potentiation    location    resolution    em    tags    coupling    chemical    visualize    vesicle    channel    paradigms    signals    inward    functions    time    contributes    triggers    ligand    compositions    lack    protein    neurons    plastic    respectively    elicits    depression    views   

Project "SINCHAIS" data sheet

The following table provides information about the project.

Coordinator		 INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA 

Organization address
address: Am Campus 1
city: KLOSTERNEUBURG
postcode: 3400
website: www.ist.ac.at

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country Austria [AT]
 Project website https://ist.ac.at/research/research-groups/shigemoto-group/erc-advanced-grant/
 Total cost 2˙481˙437 €
 EC max contribution 2˙481˙437 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2015-AdG
 Funding Scheme ERC-ADG
 Starting year 2016
 Duration (year-month-day) from 2016-07-01   to  2021-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA AT (KLOSTERNEUBURG) coordinator 2˙481˙437.00

Map

 Project objective

Ligand-gated and voltage-gated channels are key molecules in transforming chemical signals into electrical ones and electrical signals into chemical ones, respectively. At excitatory synaptic connections in the brain, activation of AMPA- and NMDA-type glutamate receptors elicits inward currents at the postsynaptic sites, and activation of voltage-gated calcium channels triggers vesicle release of glutamate in the presynaptic sites. Plastic changes in their number, location and property can lead to potentiation or depression of synaptic efficacy, alteration in time course, and coupling to effectors at both postsynaptic and presynaptic sites. These channels are all composed of distinct subunits and their compositions affect channel properties, trafficking to the synaptic sites, and interaction with associated molecules, creating a large diversity of synaptic functions. Although channels with different subunit compositions have been investigated using biochemical and electrophysiological detection methods, very little is known about single channel subunit composition in situ because of the lack of high resolution methods for analysis of protein complex in intact tissues. In this project, I will develop novel technologies to visualize subunit composition at the single channel level in individual synapses by electron microscopy, combining new EM tags, freeze-fracture replica labelling, and electron tomography. Synaptic plasticity will be induced by optogenetic stimulation of identified neurons or behavioural paradigms to examine the dynamic changes of subunit composition. Finally, physiological implications of such regulation of subunit composition will be investigated by genetic manipulation of mice combined with electrophysiological and behavioural analyses. This work will demonstrate unprecedented views of the subunit composition in situ and provide new insights into how regulation of subunit composition contributes to synaptic plasticity and animal behaviour.

 Publications

year authors and title journal last update
List of publications.
2019 Shigekazu Tabata, Marijo Jevtic, Nobutaka Kurashige, Hirokazu Fuchida, Munetsugu Kido, Kazushi Tani, Naoki Zenmyo, Shohei Uchinomiya, Harumi Harada, Makoto Itakura, Itaru Hamachi, Ryuichi Shigemoto, Akio Ojida
Electron Microscopic Detection of Single Membrane Proteins by a Specific Chemical Labeling
published pages: , ISSN: 2589-0042, DOI: 10.1016/j.isci.2019.11.025 		iScience 2020-03-20

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