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EndoSubvert SIGNED

Common mechanisms of host membrane trafficking subversion by intracellular pathogens to rupture bacterial containing vacuoles

Total Cost €

EC-Contrib. €

Partnership

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EndoSubvert project word cloud

Explore the words cloud of the EndoSubvert project. It provides you a very rough idea of what is the project "EndoSubvert" about.

listeria membrane chooses bound correlative electron discovery salmonella subversion escape fluorescence track active trafficking mechanisms scanning cytoplasmic differential strategies passive yield types biological flexneri lacks uncovered cell workflow induction shigella epithelial antimicrobial clem molecular beam enterica analyze host rab pathophysiological precise despite bacteria tuberculosis bacterial therapeutic complexity fundamental containing always definition volume ion light localization microscopy localize cascades invasion compartments rational monocytogenes rupture challenged vacuole pathogen strategy hence sensing cytoplasm mycobacterium starting model intracellular cells depending remodeling pathogens scaffolds leads integrity innovative classified regulation pathogenic understudied delineate bcv

Project "EndoSubvert" data sheet

The following table provides information about the project.

Coordinator	INSTITUT PASTEUR Organization address address: RUE DU DOCTEUR ROUX 25-28 city: PARIS CEDEX 15 postcode: 75724 website: http://www.pasteur.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	France [FR]
Total cost	2˙000˙000 €
EC max contribution	2˙000˙000 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2015-CoG
Funding Scheme	ERC-COG
Starting year	2017
Duration (year-month-day)	from 2017-01-01 to 2021-12-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	INSTITUT PASTEUR INSTITUT PASTEUR Organization address address: RUE DU DOCTEUR ROUX 25-28 city: PARIS CEDEX 15 postcode: 75724 website: http://www.pasteur.fr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	FR (PARIS CEDEX 15)	coordinator	2˙000˙000.00

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Project objective

A common strategy of bacterial pathogens is active or passive uptake into host cells. There, they can localize within a bacterial containing vacuole (BCV) or access the host cytoplasm through BCV rupture. Hence, intracellular pathogens are often classified as vacuole-bound or cytoplasmic. Recently, this definition has been challenged by the discovery that many vacuole-bound pathogens, including Mycobacterium tuberculosis and Salmonella enterica, access the host cytoplasm, and by the insight that cytoplasmic bacteria, like Shigella flexneri or Listeria monocytogenes, do not always escape the BCV. Despite this increasing complexity, a precise understanding lacks for why and how a pathogen “chooses” between a BCV or the cytoplasm and yet this is very important: because of differential pathogen sensing in membrane-bound and cytoplasmic compartments, intracellular localization leads to induction of different host responses. Therefore, a comprehensive understanding of the processes controlling BCV integrity is not only essential, but can provide new therapeutic targets. Our previous research has implemented innovative fluorescence microscopy to track the invasion steps of pathogenic bacteria. We have further integrated a large-volume, correlative, light/electron microscopy (CLEM) workflow via focused ion beam scanning electron microscopy. This uncovered the subversion of host Rab cascades by Shigella to rupture its BCV. Starting with the Shigella model of epithelial cell invasion, we will delineate the precise molecular mechanisms controlling BCV integrity in different host cell types. We will analyze (i) the scaffolds of host pathways for membrane remodeling, (ii) their subversion by various pathogens, and (iii) their differential regulation depending on pathophysiological conditions. Together, this will allow development of novel, rational antimicrobial strategies and will yield fundamental insight into understudied cell biological mechanisms of membrane trafficking.

Publications

List of publications.
year	authors and title	journal	last update
2018	Jakub Voznica, Christophe Gardella, Ilia Belotserkovsky, Alexandre Dufour, Jost Enninga, Virginie StÃ©venin Identification of Parameters of Host Cell Vulnerability during Salmonella Infection by Quantitative Image Analysis and Modeling published pages: , ISSN: 0019-9567, DOI: 10.1128/iai.00644-17	Infection and Immunity 86/1	2019-11-07
2017	Chun Hui Sun, Benjamin Wacquier, Daniel I Aguilar, Nathalie Carayol, Kevin Denis, Sylviane Boucherie, Cesar Valenciaâ€Gallardo, Ceren Simsek, Christophe Erneux, Alexandre Lehman, Jost Enninga, Laurence Arbibe, Philippe Sansonetti, GeneviÃ¨ve Dupont, Laurent Combettes, Guy Tran Van Nhieu The Shigella type III effector IpgD recodes Ca 2+ signals during invasion of epithelial cells published pages: 2567-2580, ISSN: 0261-4189, DOI: 10.15252/embj.201696272	The EMBO Journal 36/17	2019-11-07
2018	Jakub Voznica, Jost Enninga, Virginie StÃ©venin High-throughput Microscopic Analysis of Salmonella Invasion of Host Cells published pages: , ISSN: 2331-8325, DOI: 10.21769/bioprotoc.3017	BIO-PROTOCOL 8/18	2019-11-07
2019	Allon Weiner, Jost Enninga The Pathogenâ€“Host Interface in Three Dimensions: Correlative FIB/SEM Applications published pages: 426-439, ISSN: 0966-842X, DOI: 10.1016/j.tim.2018.11.011	Trends in Microbiology 27/5	2019-11-07
2016	Magdalena Gil, Jost Enninga Tracing a fat or sweet lifestyle - New insights on catabolic paths of intracellular Salmonella published pages: 655-657, ISSN: 2150-5594, DOI: 10.1080/21505594.2017.1283466	Virulence 8/6	2019-11-07
2018	Marine Oberkampf, Camille Guillerey, Juliette MouriÃ¨s, Pierre Rosenbaum, Catherine Fayolle, Alexandre Bobard, Ariel Savina, Eric Ogier-Denis, Jost Enninga, Sebastian Amigorena, Claude Leclerc, Gilles Dadaglio Mitochondrial reactive oxygen species regulate the induction of CD8+ T cells by plasmacytoid dendritic cells published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-04686-8	Nature Communications 9/1	2019-11-07
2018	Jennifer Fredlund, JosÃ© Carlos Santos, Virginie StÃ©venin, Allon Weiner, Patricia Latour-Lambert, Katya Rechav, Adeline Mallet, Jacomine Krijnse-Locker, Michael Elbaum, Jost Enninga The entry of Salmonella in a distinct tight compartment revealed at high temporal and ultrastructural resolution published pages: e12816, ISSN: 1462-5814, DOI: 10.1111/cmi.12816	Cellular Microbiology 20/4	2019-11-07
2017	Sonja KÃ¼hn, Noelia Lopez-Montero, Yuen-Yan Chang, Anna Sartori-Rupp, Jost Enninga Imaging macropinosomes during Shigella infections published pages: 12-22, ISSN: 1046-2023, DOI: 10.1016/j.ymeth.2017.05.007	Methods 127	2019-11-07

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