Explore the words cloud of the NanoCellActivity project. It provides you a very rough idea of what is the project "NanoCellActivity" about.
The following table provides information about the project.
Coordinator |
KATHOLIEKE UNIVERSITEIT LEUVEN
Organization address contact info |
Coordinator Country | Belgium [BE] |
Total cost | 1˙368˙250 € |
EC max contribution | 1˙368˙250 € (100%) |
Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
Code Call | ERC-2016-STG |
Funding Scheme | ERC-STG |
Starting year | 2017 |
Duration (year-month-day) | from 2017-02-01 to 2022-01-31 |
Take a look of project's partnership.
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1 | KATHOLIEKE UNIVERSITEIT LEUVEN | BE (LEUVEN) | coordinator | 1˙368˙250.00 |
Fluorescence microscopy is the tool of choice for live-cell imaging. Its usefulness has been further enhanced by the availability of genetically-encoded biosensors, which enable the visualisation of when and where a certain activity arises. In addition, the development of diffraction-unlimited imaging has dramatically improved the spatial resolution of fluorescence imaging. However, these techniques have had difficulty working with biosensors, largely limiting the information to the spatial location of the labels. This project seeks to develop diffraction-unlimited imaging of biosensors, creating activity maps with a diffraction-unlimited spatial resolution in living systems. I propose to meet this challenge using a two-pronged approach, focusing both on the development of labels and sensors as well as new imaging tools and strategies. Based on existing scaffolds, we will develop sensors that display strong photochromism, providing reversible fluorescence dynamics intrinsically suited to superresolution imaging. In tandem, we will develop imaging strategies that focus on robustness and work well in living systems, in exchange for a spatial resolution of a 50 to 70 nm and a temporal resolution of a few seconds or less. We will use these developments in the study of the nanoscale spatiotemporal regulation of G-protein-coupled receptor (GCPR) signalling in living systems. By extending sub-diffraction imaging to the molecular environment, this project will contribute new insights into long-standing research questions that directly involve the health and well-being of all of us, while also enabling exciting prospects for further research.
year | authors and title | journal | last update |
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2017 |
Wim Vandenberg, Peter Dedecker Effect of probe diffusion on the SOFI imaging accuracy published pages: , ISSN: 2045-2322, DOI: 10.1038/srep44665 |
Scientific Reports 7/1 | 2019-10-15 |
2019 |
Wim Vandenberg, Marcel Leutenegger, Sam Duwé, Peter Dedecker An extended quantitative model for super-resolution optical fluctuation imaging (SOFI) published pages: 25749, ISSN: 1094-4087, DOI: 10.1364/oe.27.025749 |
Optics Express 27/18 | 2019-10-15 |
2019 |
Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam Duwé, Wolfgang Hübner, Thomas Huser, Peter Dedecker, Marcel Müller Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI) published pages: 44001, ISSN: 2515-7647, DOI: 10.1088/2515-7647/ab36ae |
Journal of Physics: Photonics 1/4 | 2019-10-15 |
2019 |
A. Purohit, W. Vandenberg, T. Dertinger, D. Wöll, P. Dedecker, J. Enderlein Spatio-temporal correlation super-resolution optical fluctuation imaging published pages: 20005, ISSN: 1286-4854, DOI: 10.1209/0295-5075/125/20005 |
EPL (Europhysics Letters) 125/2 | 2019-10-15 |
2019 |
Elke De Zitter, Daniel Thédié, Viola Mönkemöller, Siewert Hugelier, Joël Beaudouin, Virgile Adam, Martin Byrdin, Luc Van Meervelt, Peter Dedecker, Dominique Bourgeois Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM published pages: 707-710, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0462-3 |
Nature Methods 16/8 | 2019-10-15 |
2017 |
Bas M. C. Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E. Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C. Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C. Kapitein Efficient switching of mCherry fluorescence using chemical caging published pages: 7013-7018, ISSN: 0027-8424, DOI: 10.1073/pnas.1617280114 |
Proceedings of the National Academy of Sciences 114/27 | 2019-10-15 |
2019 |
Sam Duwé, Peter Dedecker Optimizing the fluorescent protein toolbox and its use published pages: 183-191, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2019.04.006 |
Current Opinion in Biotechnology 58 | 2019-10-15 |
2017 |
Yves Peeters, Wim Vandenberg, Sam Duwé, Arno Bouwens, Tomáš Lukeš, Cyril Ruckebusch, Theo Lasser, Peter Dedecker Correcting for photodestruction in super-resolution optical fluctuation imaging published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-017-09666-4 |
Scientific Reports 7/1 | 2019-04-03 |
2017 |
Thijs Roebroek, Sam Duwé, Wim Vandenberg, Peter Dedecker Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization published pages: 2015, ISSN: 1422-0067, DOI: 10.3390/ijms18092015 |
International Journal of Molecular Sciences 18/9 | 2019-04-03 |
2017 |
Sam Duwé, Peter Dedecker Super-resolution imaging goes fast and deep published pages: 1042-1044, ISSN: 1548-7091, DOI: 10.1038/nmeth.4484 |
Nature Methods 14/11 | 2019-04-03 |
2017 |
S. Duwé, W. Vandenberg, P. Dedecker Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI published pages: 7242-7245, ISSN: 1359-7345, DOI: 10.1039/c7cc02344h |
Chemical Communications 53/53 | 2019-04-03 |
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