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NanoCellActivity SIGNED

Nanoscale live-cell activity sensing using smart probes and imaging

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EC-Contrib. €

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Project "NanoCellActivity" data sheet

The following table provides information about the project.

Coordinator
KATHOLIEKE UNIVERSITEIT LEUVEN 

Organization address
address: OUDE MARKT 13
city: LEUVEN
postcode: 3000
website: www.kuleuven.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Total cost 1˙368˙250 €
 EC max contribution 1˙368˙250 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2016-STG
 Funding Scheme ERC-STG
 Starting year 2017
 Duration (year-month-day) from 2017-02-01   to  2022-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KATHOLIEKE UNIVERSITEIT LEUVEN BE (LEUVEN) coordinator 1˙368˙250.00

Map

 Project objective

Fluorescence microscopy is the tool of choice for live-cell imaging. Its usefulness has been further enhanced by the availability of genetically-encoded biosensors, which enable the visualisation of when and where a certain activity arises. In addition, the development of diffraction-unlimited imaging has dramatically improved the spatial resolution of fluorescence imaging. However, these techniques have had difficulty working with biosensors, largely limiting the information to the spatial location of the labels. This project seeks to develop diffraction-unlimited imaging of biosensors, creating activity maps with a diffraction-unlimited spatial resolution in living systems. I propose to meet this challenge using a two-pronged approach, focusing both on the development of labels and sensors as well as new imaging tools and strategies. Based on existing scaffolds, we will develop sensors that display strong photochromism, providing reversible fluorescence dynamics intrinsically suited to superresolution imaging. In tandem, we will develop imaging strategies that focus on robustness and work well in living systems, in exchange for a spatial resolution of a 50 to 70 nm and a temporal resolution of a few seconds or less. We will use these developments in the study of the nanoscale spatiotemporal regulation of G-protein-coupled receptor (GCPR) signalling in living systems. By extending sub-diffraction imaging to the molecular environment, this project will contribute new insights into long-standing research questions that directly involve the health and well-being of all of us, while also enabling exciting prospects for further research.

 Publications

year authors and title journal last update
List of publications.
2017 Wim Vandenberg, Peter Dedecker
Effect of probe diffusion on the SOFI imaging accuracy
published pages: , ISSN: 2045-2322, DOI: 10.1038/srep44665
Scientific Reports 7/1 2019-10-15
2019 Wim Vandenberg, Marcel Leutenegger, Sam Duwé, Peter Dedecker
An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)
published pages: 25749, ISSN: 1094-4087, DOI: 10.1364/oe.27.025749
Optics Express 27/18 2019-10-15
2019 Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam Duwé, Wolfgang Hübner, Thomas Huser, Peter Dedecker, Marcel Müller
Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)
published pages: 44001, ISSN: 2515-7647, DOI: 10.1088/2515-7647/ab36ae
Journal of Physics: Photonics 1/4 2019-10-15
2019 A. Purohit, W. Vandenberg, T. Dertinger, D. Wöll, P. Dedecker, J. Enderlein
Spatio-temporal correlation super-resolution optical fluctuation imaging
published pages: 20005, ISSN: 1286-4854, DOI: 10.1209/0295-5075/125/20005
EPL (Europhysics Letters) 125/2 2019-10-15
2019 Elke De Zitter, Daniel Thédié, Viola Mönkemöller, Siewert Hugelier, Joël Beaudouin, Virgile Adam, Martin Byrdin, Luc Van Meervelt, Peter Dedecker, Dominique Bourgeois
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM
published pages: 707-710, ISSN: 1548-7091, DOI: 10.1038/s41592-019-0462-3
Nature Methods 16/8 2019-10-15
2017 Bas M. C. Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E. Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C. Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C. Kapitein
Efficient switching of mCherry fluorescence using chemical caging
published pages: 7013-7018, ISSN: 0027-8424, DOI: 10.1073/pnas.1617280114
Proceedings of the National Academy of Sciences 114/27 2019-10-15
2019 Sam Duwé, Peter Dedecker
Optimizing the fluorescent protein toolbox and its use
published pages: 183-191, ISSN: 0958-1669, DOI: 10.1016/j.copbio.2019.04.006
Current Opinion in Biotechnology 58 2019-10-15
2017 Yves Peeters, Wim Vandenberg, Sam Duwé, Arno Bouwens, Tomáš Lukeš, Cyril Ruckebusch, Theo Lasser, Peter Dedecker
Correcting for photodestruction in super-resolution optical fluctuation imaging
published pages: , ISSN: 2045-2322, DOI: 10.1038/s41598-017-09666-4
Scientific Reports 7/1 2019-04-03
2017 Thijs Roebroek, Sam Duwé, Wim Vandenberg, Peter Dedecker
Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization
published pages: 2015, ISSN: 1422-0067, DOI: 10.3390/ijms18092015
International Journal of Molecular Sciences 18/9 2019-04-03
2017 Sam Duwé, Peter Dedecker
Super-resolution imaging goes fast and deep
published pages: 1042-1044, ISSN: 1548-7091, DOI: 10.1038/nmeth.4484
Nature Methods 14/11 2019-04-03
2017 S. Duwé, W. Vandenberg, P. Dedecker
Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI
published pages: 7242-7245, ISSN: 1359-7345, DOI: 10.1039/c7cc02344h
Chemical Communications 53/53 2019-04-03

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