3DMUCHO
Investigation of 3D Functional Connectivity in Mouse Visual Cortex using Holography and Optogenetics
Total Cost €
0EC-Contrib. €
0Partnership
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03DMUCHO project word cloud
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Project "3DMUCHO" data sheet
The following table provides information about the project.
| Coordinator |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Organization address contact info |
| Coordinator Country | France [FR] |
| Total cost | 173˙076 € |
| EC max contribution | 173˙076 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2016 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2017 |
| Duration (year-month-day) | from 2017-04-01 to 2019-03-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS | FR (PARIS) | coordinator | 173˙076.00 |
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Project objective
Cortical neurons respond selectively to sensory features. In mouse visual cortex, convergent evidences point towards stronger connectivity between neurons sharing similar feature selectivity in the superficial layers. The relationship between a three-dimensional (3D) subnetwork, where neurons connect horizontally and vertically to each other, and the functional specificity of individual neurons remain however unresolved. The main objective of this interdisciplinary project is to uncover the 3D functional connectivity in mouse visual cortex in vivo by employing the innovative optical methods pioneered in the host laboratory. Single-cell activation deep into the brain will be, for the first time, enabled using the novel technology of 3D depth-resolved holographic light-patterning and two-photon optogenetics. Combined with whole-cell recordings for measuring postsynaptic responses, precise presynaptic excitation with holography will permit mapping connection pairs within and across cortical planes. Simultaneous presynaptic multi-cell excitation of connecting neurons will test the integration linearity of the postsynaptic neuron. To probe functional connectivity, connection pairs will be examined from a functionally-identified neuronal population, whose tuning specificity to visual attributes is determined using calcium imaging. This fellowship will render me opportunities to acquire a versatile optical toolbox that is of general interest in neuroscience for connectivity mapping and circuit manipulation. Knowledge of local functional connectivity spanning a 3D cortical volume will further provide significant insights into the network mechanism underlying sensory processing.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2018 |
I-Wen Chen, Eirini Papagiakoumou, Valentina Emiliani Towards circuit optogenetics published pages: 179-189, ISSN: 0959-4388, DOI: 10.1016/j.conb.2018.03.008 |
Current Opinion in Neurobiology 50 | 2019-09-25 |
| 2019 |
I-Wen Chen, Emiliano Ronzitti, Brian R. Lee, Tanya L. Daigle, Deniz Dalkara, Hongkui Zeng, Valentina Emiliani, Eirini Papagiakoumou In vivo sub-millisecond two-photon optogenetics with temporally focused patterned light published pages: 1785-18, ISSN: 0270-6474, DOI: 10.1523/jneurosci.1785-18.2018 |
The Journal of Neuroscience | 2019-09-25 |
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