Opendata, web and dolomites

CellKarma SIGNED

Dissecting the regulatory logic of cell fate reprogramming through integrative and single cell genomics

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 CellKarma project word cloud

Explore the words cloud of the CellKarma project. It provides you a very rough idea of what is the project "CellKarma" about.

utilize    revolutionized    expose    genomic    primary    barriers    regulators    acquire    explore    decisions    promoters    therapies    fate    conversion    chance    regulatory    multiple    ipsc    driving    cells    transitions    ipscs    full    isolate    strategies    unlock    discovery    vitro    developmental    edge    single    medicine    ultimate    relationships    reconstruct    cutting    right    transcription    otherwise    stem    derivation    human    dissect    logic    directions    shaping    significantly    cocktail    enhancer    identity    molecular    lineage    responsible    modulate    regions    summary    stimulated    technologies    multifaceted    light    patient    modulators    cell    pluripotency    reprogramming    genome    editing    unidentified    transcriptomics    successfully    intermediates    never    synthetic    cellular    limited    rules    combines    determinants    regenerative    alternative    mutagenesis    quality    pluripotent    integrative    biology    pressing    events    transcriptional   

Project "CellKarma" data sheet

The following table provides information about the project.

Coordinator
FONDAZIONE TELETHON 

Organization address
address: VIA VARESE 16/B
city: ROMA
postcode: 185
website: www.telethon.it

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Italy [IT]
 Total cost 1˙497˙250 €
 EC max contribution 1˙497˙250 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-03-01   to  2023-02-28

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    FONDAZIONE TELETHON IT (ROMA) coordinator 1˙497˙250.00

Map

 Project objective

The concept that any cell type, upon delivery of the right “cocktail” of transcription factors, can acquire an identity that otherwise it would never achieve, revolutionized the way we approach the study of developmental biology. In light of this, the discovery of induced pluripotent stem cells (IPSCs) and cell fate conversion approaches stimulated new research directions into human regenerative biology. However, the chance to successfully develop patient-tailored therapies is still very limited because reprogramming technologies are applied without a comprehensive understanding of the molecular processes involved. Here, I propose a multifaceted approach that combines a wide range of cutting-edge integrative genomic strategies to significantly advance our understanding of the regulatory logic driving cell fate decisions during human reprogramming to pluripotency. To this end, I will utilize single cell transcriptomics to isolate reprogramming intermediates, reconstruct their lineage relationships and define transcriptional regulators responsible for the observed transitions (AIM 1). Then, I will dissect the rules by which transcription factors modulate the activity of promoters and enhancer regions during reprogramming transitions, by applying synthetic biology and genome editing approaches (AIM 2). Then, I will adopt an alternative approach to identify reprogramming modulators by the analysis of reprogramming-induced mutagenesis events (AIM 3). Finally, I will explore my findings in multiple primary reprogramming approaches to pluripotency, with the ultimate goal of improving the quality of IPSC derivation (Aim 4). In summary, this project will expose novel determinants and yet unidentified molecular barriers of reprogramming to pluripotency and will be essential to unlock the full potential of reprogramming technologies for shaping cellular identity in vitro and to address pressing challenges of regenerative medicine.

 Publications

year authors and title journal last update
List of publications.
2018 Davide Cacchiarelli, Xiaojie Qiu, Sanjay Srivatsan, Anna Manfredi, Michael Ziller, Eliah Overbey, Antonio Grimaldi, Jonna Grimsby, Prapti Pokharel, Kenneth J. Livak, Shuqiang Li, Alexander Meissner, Tarjei S. Mikkelsen, John L. Rinn, Cole Trapnell
Aligning Single-Cell Developmental and Reprogramming Trajectories Identifies Molecular Determinants of Myogenic Reprogramming Outcome
published pages: 258-268.e3, ISSN: 2405-4712, DOI: 10.1016/j.cels.2018.07.006
Cell Systems 7/3 2019-11-07
2018 Marcella Cesana, Michael H. Guo, Davide Cacchiarelli, Lara Wahlster, Jessica Barragan, Sergei Doulatov, Linda T. Vo, Beatrice Salvatori, Cole Trapnell, Kendell Clement, Patrick Cahan, Kaloyan M. Tsanov, Patricia M. Sousa, Barbara Tazon-Vega, Adriano Bolondi, Federico M. Giorgi, Andrea Califano, John L. Rinn, Alexander Meissner, Joel N. Hirschhorn, George Q. Daley
A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development
published pages: 575-588.e7, ISSN: 1934-5909, DOI: 10.1016/j.stem.2018.03.012
Cell Stem Cell 22/4 2019-11-07

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "CELLKARMA" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "CELLKARMA" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

RECON (2019)

Reprogramming Conformation by Fluorination: Exploring New Areas of Chemical Space

Read More  

EAST (2020)

Using Evolutionary Algorithms to Understand and Secure Web/Enterprise Systems

Read More  

URBAG (2019)

Integrated System Analysis of Urban Vegetation and Agriculture

Read More