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CRISPR-Locate SIGNED

Linking sequence to function of long noncoding RNAs with CRISPR.

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 CRISPR-Locate project word cloud

Explore the words cloud of the CRISPR-Locate project. It provides you a very rough idea of what is the project "CRISPR-Locate" about.

rnas    purify    presently    place    functional    estimates    context    biological    resource    delete    mature    tagged    separable    handful    sequence    time    human    faster       insert    created    encoded    medicine    lncrna    detecting    discovering    natural    hypothesis    lncrnas    vast    contains    localization    subcellular    least    map    molecules       experiments    molecule    create    surprise    endogenous    wild    throughput    wake    fractionate    locate    genes    subsequently    proxy    rna    19    biology    tags    annotation    invaluable    linking    question    pressing    cells    significance    laborious    10    responsible    experimentally    compartments    noncoding    extensive    afterwards    discoveries    localisation    proteins    closer    function    coding    characterised    crispr    mutated    primary    1000    grand    lt    encode    functions    alongside    efforts    domains    protein    technique    genome    discovery    deletion    push    modular    elucidated    simultaneously    conventional    unlocking   

Project "CRISPR-Locate" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAET BERN 

Organization address
address: HOCHSCHULSTRASSE 6
city: BERN
postcode: 3012
website: http://www.unibe.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 191˙149 €
 EC max contribution 191˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2021
 Duration (year-month-day) from 2021-07-01   to  2023-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET BERN CH (BERN) coordinator 191˙149.00

Map

 Project objective

A great surprise in the wake of the Human Genome Project has been the discovery of vast numbers of RNAs that do not encode proteins. Alongside 19,000 protein-coding genes, our genome contains at least 20,000 long noncoding RNA (lncRNA) genes, but recent estimates push that number to 100,000. Extensive annotation efforts are presently discovering lncRNAs far faster than their functions can be elucidated, and thus only <1% of lncRNAs have been experimentally characterised. These discoveries have created a grand challenge of understanding lncRNAs’ biological significance. To do this, we must solve the pressing question of how lncRNAs’ functions are encoded in their primary sequence. As a proxy of lncRNAs function we can use subcellular localisation since lncRNAs function as a mature RNA molecule. One hypothesis is that, similar to proteins, lncRNAs are modular molecules composed of separable functional domains. Previous studies, including my own, have used conventional methods to identify domains in a handful of lncRNAs by laborious deletion experiments. I propose to advance this field, via a novel high-throughput technique, CRISPR-Locate, capable of detecting lncRNA domains and their function in their natural endogenous context. I will delete ⁓1000 different lncRNA domains at the same time and simultaneously insert RNA tags at their place. Subsequently, I will fractionate cells by their compartments and purify tagged lncRNAs. Afterwards, I will sequence all the tagged lncRNAs and, by comparing the change in the subcellular localisation between mutated and wild type cells, I will identify which domains are responsible for subcellular localization. Finally, I will use CRISPR-Locate to create a map of lncRNA domains, an invaluable resource linking sequence to function, and bring us a step closer to unlocking the potential of 10^4 novel genes in medicine and biology.

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The information about "CRISPR-LOCATE" are provided by the European Opendata Portal: CORDIS opendata.

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