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CRISPR-Locate SIGNED

Linking sequence to function of long noncoding RNAs with CRISPR.

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 CRISPR-Locate project word cloud

Explore the words cloud of the CRISPR-Locate project. It provides you a very rough idea of what is the project "CRISPR-Locate" about.

hypothesis    characterised    map    endogenous    create    natural    simultaneously    handful    least    lt    insert    delete    functional    tagged    localization    encode    biological    function    molecule    lncrna    grand    extensive    10    question    localisation    linking    conventional    estimates    created    closer    wake    crispr    mutated    elucidated    genes    mature    human    coding    laborious    biology    alongside    surprise    rnas    domains    modular    contains    responsible    significance    vast    locate    molecules    context    invaluable    afterwards    tags    discovery    sequence    discoveries    resource    1000    protein    deletion    primary    separable    medicine    technique    encoded       rna    subsequently    proteins    pressing    wild    push    discovering    fractionate    detecting    unlocking    experiments    proxy    efforts       noncoding    subcellular    annotation    throughput    place    faster    experimentally    lncrnas    19    functions    presently    purify    genome    cells    compartments    time   

Project "CRISPR-Locate" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITAET BERN 

Organization address
address: HOCHSCHULSTRASSE 6
city: BERN
postcode: 3012
website: http://www.unibe.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 191˙149 €
 EC max contribution 191˙149 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2019
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2021
 Duration (year-month-day) from 2021-07-01   to  2023-06-30

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITAET BERN CH (BERN) coordinator 191˙149.00

Map

 Project objective

A great surprise in the wake of the Human Genome Project has been the discovery of vast numbers of RNAs that do not encode proteins. Alongside 19,000 protein-coding genes, our genome contains at least 20,000 long noncoding RNA (lncRNA) genes, but recent estimates push that number to 100,000. Extensive annotation efforts are presently discovering lncRNAs far faster than their functions can be elucidated, and thus only <1% of lncRNAs have been experimentally characterised. These discoveries have created a grand challenge of understanding lncRNAs’ biological significance. To do this, we must solve the pressing question of how lncRNAs’ functions are encoded in their primary sequence. As a proxy of lncRNAs function we can use subcellular localisation since lncRNAs function as a mature RNA molecule. One hypothesis is that, similar to proteins, lncRNAs are modular molecules composed of separable functional domains. Previous studies, including my own, have used conventional methods to identify domains in a handful of lncRNAs by laborious deletion experiments. I propose to advance this field, via a novel high-throughput technique, CRISPR-Locate, capable of detecting lncRNA domains and their function in their natural endogenous context. I will delete ⁓1000 different lncRNA domains at the same time and simultaneously insert RNA tags at their place. Subsequently, I will fractionate cells by their compartments and purify tagged lncRNAs. Afterwards, I will sequence all the tagged lncRNAs and, by comparing the change in the subcellular localisation between mutated and wild type cells, I will identify which domains are responsible for subcellular localization. Finally, I will use CRISPR-Locate to create a map of lncRNA domains, an invaluable resource linking sequence to function, and bring us a step closer to unlocking the potential of 10^4 novel genes in medicine and biology.

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The information about "CRISPR-LOCATE" are provided by the European Opendata Portal: CORDIS opendata.

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