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NewSpindleForce SIGNED

A new class of microtubules in the spindle exerting forces on kinetochores

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

NewSpindleForce project word cloud

Explore the words cloud of the NewSpindleForce project. It provides you a very rough idea of what is the project "NewSpindleForce" about.

chromosomes anticancer cells perturbations erc strength expertise groundbreaking responsible positioning international laser question onset leader division pole forces link techniques hypothesis thereby preliminary machine proteins models fibers mechanism strategies edge forms chemotherapy interdisciplinary movements position at foremost kinetochore combining uncover divide extensive generation microsurgery cell kinetochores me pulling bridging accurate theoretical shed micro sister made bundles force quantitative strengthen experiments ptk1 microscopy microtubules bridge genetic interactions mitotic movement segregation central light class hela spindle organization cutting complexes paradigm chromosome protein

Project "NewSpindleForce" data sheet

The following table provides information about the project.

Coordinator	RUDER BOSKOVIC INSTITUTE Organization address address: Bijenicka cesta 54 city: ZAGREB postcode: 10000 website: www.irb.hr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Croatia [HR]
Project website	http://tolic.irb.hr/erc-project
Total cost	2˙150˙000 €
EC max contribution	2˙150˙000 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2014-CoG
Funding Scheme	ERC-COG
Starting year	2015
Duration (year-month-day)	from 2015-04-01 to 2020-03-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	RUDER BOSKOVIC INSTITUTE RUDER BOSKOVIC INSTITUTE Organization address address: Bijenicka cesta 54 city: ZAGREB postcode: 10000 website: www.irb.hr contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	HR (ZAGREB)	coordinator	2˙150˙000.00

Map

Project objective

At the onset of division the cell forms a spindle, a micro-machine made of microtubules, which divide the chromosomes by pulling on kinetochores, protein complexes on the chromosome. The central question in the field is how accurate chromosome segregation results from the interactions between kinetochores, microtubules and the associated proteins. According to the current paradigm, the forces on kinetochores are produced by k-fibers, bundles of microtubules extending between the spindle pole and the kinetochore. The proposed project is built upon a groundbreaking hypothesis that a new class of microtubules, which we term bridging microtubules, bridge sister kinetochores. Our preliminary results show that bridging microtubules are responsible for the positioning of kinetochores in HeLa and PtK1 cells. Bridging microtubules have not been studied before because this requires cutting-edge microscopy and laser microsurgery techniques. By applying these methods, with which I have extensive expertise, we will determine the organization of these microtubules, identify the proteins that link them with k-fibers, and uncover where and how the forces for kinetochore positioning and movement are generated. My strength is in taking an interdisciplinary approach, which I will use in this project by combining laser microsurgery with genetic perturbations, quantitative measurements of the responses and comparison with theoretical models. Understanding the role of bridging microtubules in force generation and chromosome movements will not only shed light on the mechanism of chromosome segregation, but may also increase the potential of mitotic anticancer strategies, as the spindle is a major target for chemotherapy. The proposed ERC funding is essential for the success of these timely and ambitious experiments, allowing me to strengthen my position as an international leader in research on cell division, thereby increasing Europe's foremost position in this field.

Publications

List of publications.
year	authors and title	journal	last update
2019	Iva M. ToliÄ‡, Maja Novak, Nenad Pavin Helical Twist and Rotational Forces in the Mitotic Spindle published pages: 132, ISSN: 2218-273X, DOI: 10.3390/biom9040132	Biomolecules 9/4	2019-08-06
2016	Iva M. ToliÄ‡, Nenad Pavin Bridging the gap between sister kinetochores published pages: 1169-1170, ISSN: 1538-4101, DOI: 10.1080/15384101.2016.1157976	Cell Cycle 15/9	2019-05-31
2017	Bruno Polak, Patrik Risteski, Sonja Lesjak, Iva M ToliÄ‡ PRC1â€labeled microtubule bundles and kinetochore pairs show oneâ€toâ€one association in metaphase published pages: 217-230, ISSN: 1469-221X, DOI: 10.15252/embr.201642650	EMBO reports 18/2	2019-05-31
2017	Iva M. ToliÄ‡ Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles published pages: , ISSN: 0175-7571, DOI: 10.1007/s00249-017-1244-4	European Biophysics Journal	2019-05-31
2015	Nenad Pavin, Iva M. ToliÄ‡ Self-Organization and Forces in the Mitotic Spindle published pages: 279-298, ISSN: 1936-122X, DOI: 10.1146/annurev-biophys-062215-010934	Annual Review of Biophysics 45/1	2019-05-31
2016	Janko Kajtez, Anastasia Solomatina, Maja Novak, Bruno Polak, Kruno VukuÅ¡iÄ‡, Jonas RÃ¼diger, Gheorghe Cojoc, Ana Milas, Ivana Å umanovac Å estak, Patrik Risteski, Federica Tavano, Anna H. Klemm, Emanuele Roscioli, Julie Welburn, Daniela Cimini, Matko GlunÄiÄ‡, Nenad Pavin, Iva M. ToliÄ‡ Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores published pages: 10298, ISSN: 2041-1723, DOI: 10.1038/ncomms10298	Nature Communications 7	2019-05-31
2018	Anna H. Klemm, Agneza Bosilj, Matko GluncË‡icÂ´, Nenad Pavin, Iva M. TolicÂ´ Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability published pages: 1332-1345, ISSN: 1059-1524, DOI: 10.1091/mbc.e17-11-0667	Molecular Biology of the Cell 29/11	2019-05-31
2017	R. BuÄ‘a, K. VukuÅ¡iÄ‡, I.M. ToliÄ‡ Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation. published pages: 81-101, ISSN: , DOI: 10.1016/bs.mcb.2016.11.007	Methods in Cell Biology 139: 81â€“101 (2017)	2019-05-31
2016	Ana Milas, Iva ToliÄ‡ Relaxation of interkinetochore tension after severing of a k-fiber depends on the length of the k-fiber stub published pages: , ISSN: 2297-9239, DOI: 10.19185/matters.201603000025	Matters Select Mar 23rd, 2016	2019-05-31

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