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TOTIPOTENCY2014

Dissecting the epigenetic control of totipotency.

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EC-Contrib. €

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Partnership

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 Outcomes and results

 TOTIPOTENCY2014 project word cloud

Explore the words cloud of the TOTIPOTENCY2014 project. It provides you a very rough idea of what is the project "TOTIPOTENCY2014" about.

mechanisms    gene    researcher    bioinformatic    data    lab    edge    innovative    systematically    extra    sub    additional    efficiency    cells    34    proteins    critical    expressing    technologies    interdependencies    screened    totipotency    lack    experimentally    poorly    normally    am    interaction    analysed    mode    restricted    host    differentiation    model    expertise    somatic    regulators    rare    restoring    regulation    occurs    epigenetic    stem    escs    mechanistically    genes    contribution    shortlisted    therapy    enhancers    fidelity    transcriptome    cell    expression    action    personalised    limited    tractable    implantation    validated    epigenetics    potentially    developmental    enhanced    regenerative    mammalian    embryonic    vitro    combines    cutting    stage    determined    reprogramming    single    confirmed    biology    uses    fertilization    newly    chromatin    firstly    medicine    insights    murine    contain    expands    validating    previously    relevance    enhancer    remodelling    capability    population    embryo    one    shortly    eel    mechanistic   

Project "TOTIPOTENCY2014" data sheet

The following table provides information about the project.

Coordinator		 THE BABRAHAM INSTITUTE 

Organization address
address: Babraham Hall
city: CAMBRIDGE
postcode: CB22 3AT
website: www.babraham.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.
 Coordinator Country United Kingdom [UK]
 Project website https://www.babraham.ac.uk/our-research/epigenetics/wolf-reik
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2014
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-01-01   to  2017-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    THE BABRAHAM INSTITUTE UK (CAMBRIDGE) coordinator 183˙454.00

Map

 Project objective

One of the most critical epigenetic and chromatin remodelling processes in mammalian development occurs shortly after fertilization restoring totipotency. Due to limited cell numbers and lack of experimentally tractable systems, the mechanisms and regulation of this developmental stage are poorly understood. This proposal will provide important mechanistic insights into the epigenetic control of early embryonic gene expression, and its relevance to somatic reprogramming.

Murine embryonic stem cells (ESCs) contain a rare sub-population of early embryonic like (EEL) cells expressing genes normally restricted to the pre-implantation embryo, with enhanced extra-embryonic differentiation capability. Firstly, using novel single-cell technologies, I will characterize the EEL cells in detail, validating them as an in vitro model of pre-implantation development. On-going research in the host lab that I am involved in has identified an epigenetic enhancer that expands this EEL sub-population. Through bioinformatic analysis of pre-implantation transcriptome data, I have shortlisted 34 epigenetic and chromatin-associated proteins, including the previously identified factor, which will be systematically screened to identify new enhancers of totipotency. Validated EEL regulators, including two additional newly confirmed factors, will be analysed mechanistically determining their interaction partners, interdependencies, and mode of action. Finally, the contribution of the totipotency regulators towards the efficiency and fidelity of somatic reprogramming will be determined, potentially improving the use of this technology for personalised gene therapy and regenerative medicine.

This state-of-the-art proposal uses innovative and novel cutting-edge technologies and combines the expertise of the researcher and the host. It has the potential for significant impact across several fields from epigenetics and stem cell biology to reprogramming and regenerative medicine.

 Publications

year authors and title journal last update
List of publications.
2016 Mélanie A. Eckersley-Maslin, Valentine Svensson, Christel Krueger, Thomas M. Stubbs, Pascal Giehr, Felix Krueger, Ricardo J. Miragaia, Charalampos Kyriakopoulos, Rebecca V. Berrens, Inês Milagre, Jörn Walter, Sarah A. Teichmann, Wolf Reik
MERVL/Zscan4 Network Activation Results in Transient Genome-wide DNA Demethylation of mESCs
published pages: 179-192, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2016.08.087 		Cell Reports 17/1 2019-06-14

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