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RNA Transport and Control

Mechanisms of kinesin-dependent RNA transport and translational regulation

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

RNA Transport and Control project word cloud

Explore the words cloud of the RNA Transport and Control project. It provides you a very rough idea of what is the project "RNA Transport and Control" about.

coupled experimental ncrnas severe neurons noncoding literature framework combined microfluidics reconstitution diseases principles techniques linked laboratory translational misregulation time dual unsolved herein localised mechanistic plasticity synaptic answer mrnas imaging unpublished spatiotemporal dendritically translation memory anwswer independent vitro dependent gene questions localisation rbps mrna motor rnas local unravel biochemical rna effect simultaneously reveal mechanistically function motility expression kinesin regulators additionally reconstitute tia act neuronal regulation dendritic proteins binding transport connected active components mechanisms candidate assay fundamental host

Project "RNA Transport and Control" data sheet

The following table provides information about the project.

Coordinator	FUNDACIO CENTRE DE REGULACIO GENOMICA Organization address address: CARRER DOCTOR AIGUADER 88 city: BARCELONA postcode: 8003 website: www.crg.es contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Spain [ES]
Project website	http://www.crg.eu/en/sebastian_maurer
Total cost	158˙121 €
EC max contribution	158˙121 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2014
Funding Scheme	MSCA-IF-EF-ST
Starting year	2015
Duration (year-month-day)	from 2015-06-01 to 2017-05-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	FUNDACIO CENTRE DE REGULACIO GENOMICA FUNDACIO CENTRE DE REGULACIO GENOMICA Organization address address: CARRER DOCTOR AIGUADER 88 city: BARCELONA postcode: 8003 website: www.crg.es contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	ES (BARCELONA)	coordinator	158˙121.00

Map

Project objective

Active mRNA transport and localisation are crucial for spatiotemporal control of gene expression. In neurons, dendritic mRNA transport and local translational control are required for synaptic plasticity and memory formation. Misregulation has been linked to severe diseases. RNA-binding proteins (RBPs), kinesin motor proteins, mRNAs and noncoding RNAs (ncRNAs) are involved in dendritic mRNA transport and translational regulation. Still it is not known which essential set of factors is required to enable kinesin-dependent mRNA transport. Further, many RBPs required for mRNA transport simultaneously act as regulators of translation. How these crucial activities are mechanistically linked is unsolved. To anwswer these questions, this project aims to reveal essential mechanisms by two independent biochemical in vitro reconstitution approaches: (1) I will reconstitute kinesin-dependent transport of a dendritically localised mRNA and noncoding RNA using candidate proteins and RNAs from literature and an unpublished result of the host laboratory. (2) I will analyse the dual function of RBPs in mRNA transport and translational regulation. To this end, a microfluidics-coupled in vitro translation imaging assay (TIA) will be developed to study the effect of RBPs and ncRNAs on translation in real-time. Finally, the in vitro motility assay will be combined with the TIA to investigate the impact of translational regulation on mRNA transport. Using these techniques I will address key questions: (i) Which are the essential components required for kinesin-dependent mRNA transport? (ii) How are mRNA transport and translational regulation mechanistically connected? This project aims to unravel fundamental principles, improving our knowledge of neuronal mRNA transport and gene expression on a mechanistic level. Additionally, the experimental framework developed herein will enable the field to answer further important questions relating to the growing field of localised translation.

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The information about "RNA TRANSPORT AND CONTROL" are provided by the European Opendata Portal: CORDIS opendata.