NeuroInCellNMR SIGNED
In-cell NMR monitoring of alpha-Synuclein aggregation in neuronal cells
Total Cost €
0EC-Contrib. €
0Partnership
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Explore the words cloud of the NeuroInCellNMR project. It provides you a very rough idea of what is the project "NeuroInCellNMR" about.
Project "NeuroInCellNMR" data sheet
The following table provides information about the project.
| Coordinator |
WEIZMANN INSTITUTE OF SCIENCE
Organization address contact info |
| Coordinator Country | Israel [IL] |
| Total cost | 1˙996˙500 € |
| EC max contribution | 1˙996˙500 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2014-CoG |
| Funding Scheme | ERC-COG |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-11-01 to 2021-02-28 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | WEIZMANN INSTITUTE OF SCIENCE | IL (REHOVOT) | coordinator | 1˙143˙821.00 |
| 2 | FORSCHUNGSVERBUND BERLIN EV | DE (BERLIN) | participant | 852˙678.00 |
Map
Project objective
Intracellular aggregation of the human amyloid protein alpha-synuclein is causally involved in Parkinson’s disease, a debilitating neurodegenerative disorder. The goal of this project is to combine low-resolution, fluorescence-imaging methods with high-resolution in-cell NMR and EPR spectroscopy techniques to derive macroscopic and microscopic insights into alpha-synuclein aggregate structures directly in neuronal cells.
To achieve this goal, we will employ different sets of cultured neurons and investigate intracellular alpha-synuclein aggregation under defined conditions of mitochondrial dysfunction and cellular oxidative stress, two of the most common denominators of the disease. Importantly, we will also establish a human stem cell model for studying alpha-synuclein aggregation with high-resolution in-cell NMR and EPR methods, by using induced pluripotent stem cell (iPSC) derived dopaminergic neurons from Parkinson’s disease patients and control individuals.
Results from this study will provide novel insights into the native mechanisms of intracellular aggregate formation and ultimately enable novel pharmacological approaches for therapeutic intervention.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2016 |
Thomas Müntener, Daniel Häussinger, Philipp Selenko, Francois-Xavier Theillet In-Cell Protein Structures from 2D NMR Experiments published pages: 2821-2825, ISSN: 1948-7185, DOI: 10.1021/acs.jpclett.6b01074 |
The Journal of Physical Chemistry Letters 7/14 | 2019-07-25 |
| 2016 |
A. Mylona, F.-X. Theillet, C. Foster, T. M. Cheng, F. Miralles, P. A. Bates, P. Selenko, R. Treisman Opposing effects of Elk-1 multisite phosphorylation shape its response to ERK activation published pages: 233-237, ISSN: 0036-8075, DOI: 10.1126/science.aad1872 |
Science 354/6309 | 2019-07-25 |
| 2016 |
Andres Binolfi, Antonio Limatola, Silvia Verzini, Jonas Kosten, Francois-Xavier Theillet, Honor May Rose, Beata Bekei, Marchel Stuiver, Marleen van Rossum, Philipp Selenko Intracellular repair of oxidation-damaged α-synuclein fails to target C-terminal modification sites published pages: 10251, ISSN: 2041-1723, DOI: 10.1038/ncomms10251 |
Nature Communications 7 | 2019-07-25 |
| 2017 |
Jürgen M Plitzko, Benjamin Schuler, Philipp Selenko Structural Biology outside the box — inside the cell published pages: 110-121, ISSN: 0959-440X, DOI: 10.1016/j.sbi.2017.06.007 |
Current Opinion in Structural Biology 46 | 2019-07-25 |
| 2018 |
Gavin Young, Nikolas Hundt, Daniel Cole, Adam Fineberg, Joanna Andrecka, Andrew Tyler, Anna Olerinyova, Ayla Ansari, Erik G. Marklund, Miranda P. Collier, Shane A. Chandler, Olga Tkachenko, Joel Allen, Max Crispin, Neil Billington, Yasuharu Takagi, James R. Sellers, Cédric Eichmann, Philipp Selenko, Lukas Frey, Roland Riek, Martin R. Galpin, Weston B. Struwe, Justin L. P. Benesch, Philipp Kukura Quantitative mass imaging of single biological macromolecules published pages: 423-427, ISSN: 0036-8075, DOI: 10.1126/science.aar5839 |
Science 360/6387 | 2019-07-25 |
| 2016 |
Francois-Xavier Theillet, Andres Binolfi, Beata Bekei, Andrea Martorana, Honor May Rose, Marchel Stuiver, Silvia Verzini, Dorothea Lorenz, Marleen van Rossum, Daniella Goldfarb, Philipp Selenko Structural disorder of monomeric α-synuclein persists in mammalian cells published pages: 45-50, ISSN: 0028-0836, DOI: 10.1038/nature16531 |
Nature 530/7588 | 2019-07-25 |
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