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BrightSens SIGNED
Ultrabright Turn-on Fluorescent Organic Nanoparticles for Amplified Molecular Sensing in Living Cells
Total Cost €
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0BrightSens project word cloud
Explore the words cloud of the BrightSens project. It provides you a very rough idea of what is the project "BrightSens" about.
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Project "BrightSens" data sheet
The following table provides information about the project.
| Coordinator |
UNIVERSITE DE STRASBOURG
Organization address contact info |
| Coordinator Country | France [FR] |
| Project website | http://www-lbp.unistra.fr/rubrique78.html |
| Total cost | 1˙999˙750 € |
| EC max contribution | 1˙999˙750 € (100%) |
| Programme |
1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC)) |
| Code Call | ERC-2014-CoG |
| Funding Scheme | ERC-COG |
| Starting year | 2015 |
| Duration (year-month-day) | from 2015-06-01 to 2020-05-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | UNIVERSITE DE STRASBOURG | FR (STRASBOURG) | coordinator | 1˙999˙750.00 |
Map
Project objective
Existing fluorescent molecular probes, due to limited brightness, do not allow imaging individual biomolecules directly in living cells, whereas bright fluorescent nanoparticles are unable to respond to single molecular stimuli and their inorganic core is not biodegradable. The aim of BrightSens is to develop ultrabright fluorescent organic nanoparticles (FONs) capable to convert single molecular stimuli into collective turn-on response of >100 encapsulated dyes, and to apply them in amplified molecular sensing of specific targets at the cell surface (receptors) and in the cytosol (mRNA). The project is composed of three work packages. (1) Synthesis of FONs: Dye-doped polymer and micellar FONs will be obtained by self-assembly. Molecular design of dyes and the use of bulky hydrophobic counterions will enable precise control of dyes organization inside FONs, which will resolve the fundamental problems of self-quenching and cooperative on/off switching in dye ensembles. (2) Synthesis of nanoprobes: Using cooperative Forster Resonance Energy Transfer from FONs to originally designed acceptor-sensor unit, we propose synthesis of the first nanoprobes that (a) undergo complete turn-on or colour switch in response to single molecular targets and (b) harvest light energy into photochemical disruption of cell membrane barriers. (3) Cellular applications: The obtained nanoprobes will be applied in 2D and 3D cultures of cancer cells for background-free single-molecule detection of membrane receptors and intracellular mRNA, which are important markers of cancer and apoptosis. An original concept of amplified photochemical internalization is proposed to trigger by light entry of nanoprobes into the cytosol. This high-risk/high-gain multidisciplinary project will result in new organic nanomaterials with unique photophysical properties that will enable visualization of biomolecules at work in living cells with expected impact on cancer research.
Publications
| year | authors and title | journal | last update |
|---|---|---|---|
| 2018 |
Tkhe Fam, Andrey Klymchenko, Mayeul Collot Recent Advances in Fluorescent Probes for Lipid Droplets published pages: 1768, ISSN: 1996-1944, DOI: 10.3390/ma11091768 |
Materials 11/9 | 2020-02-19 |
| 2019 |
Xinyue Wang, Nicolas Anton, Pichandi Ashokkumar, Halina Anton, Tkhe Kyong Fam, Thierry Vandamme, Andrey S. Klymchenko, Mayeul Collot Optimizing the Fluorescence Properties of Nanoemulsions for Single Particle Tracking in Live Cells published pages: 13079-13090, ISSN: 1944-8244, DOI: 10.1021/acsami.8b22297 |
ACS Applied Materials & Interfaces 11/14 | 2020-02-19 |
| 2018 |
Redouane Bouchaala, Ludovic Richert, Nicolas Anton, Thierry F. Vandamme, Smail Djabi, Yves Mély, Andrey S. Klymchenko Quantifying Release from Lipid Nanocarriers by Fluorescence Correlation Spectroscopy published pages: 14333-14340, ISSN: 2470-1343, DOI: 10.1021/acsomega.8b01488 |
ACS Omega 3/10 | 2020-02-19 |
| 2020 |
Nina Melnychuk, Sylvie Egloff, Anne Runser, Andreas Reisch, Andrey S Klymchenko Light-harvesting nanoparticle probes for FRET-based detection of oligonucleotides with single-molecule sensitivity published pages: , ISSN: 1433-7851, DOI: 10.1002/anie.201913804 |
Angewandte Chemie International Edition | 2020-02-19 |
| 2019 |
Mayeul Collot, Pichandi Ashokkumar, Halina Anton, Emmanuel Boutant, Orestis Faklaris, Thierry Galli, Yves Mély, Lydia Danglot, Andrey S. Klymchenko MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience published pages: 600-614.e7, ISSN: 2451-9456, DOI: 10.1016/j.chembiol.2019.01.009 |
Cell Chemical Biology 26/4 | 2020-02-19 |
| 2019 |
Pichandi Ashokkumar, Anila Hoskere Ashoka, Mayeul Collot, Amitava Das, Andrey S. Klymchenko A fluorogenic BODIPY molecular rotor as an apoptosis marker published pages: 6902-6905, ISSN: 1359-7345, DOI: 10.1039/c9cc03242h |
Chemical Communications 55/48 | 2020-02-19 |
| 2020 |
Farah Bouhedda, Kyong Tkhe Fam, Mayeul Collot, Alexis Autour, Stefano Marzi, Andrey Klymchenko, Michael Ryckelynck A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells published pages: 69-76, ISSN: 1552-4450, DOI: 10.1038/s41589-019-0381-8 |
Nature Chemical Biology 16/1 | 2020-02-19 |
| 2019 |
Nagappanpillai Adarsh, Andrey S. Klymchenko Ionic aggregation-induced emission dye with bulky counterions for preparation of bright near-infrared polymeric nanoparticles published pages: 13977-13987, ISSN: 2040-3364, DOI: 10.1039/c9nr04085d |
Nanoscale 11/29 | 2020-02-19 |
| 2018 |
Nina Melnychuk, Andrey S. Klymchenko DNA-Functionalized Dye-Loaded Polymeric Nanoparticles: Ultrabright FRET Platform for Amplified Detection of Nucleic Acids published pages: 10856-10865, ISSN: 0002-7863, DOI: 10.1021/jacs.8b05840 |
Journal of the American Chemical Society 140/34 | 2020-02-19 |
| 2019 |
Mayeul Collot, Sophie Bou, Tkhe Kyong Fam, Ludovic Richert, Yves Mély, Lydia Danglot, Andrey S. Klymchenko Probing Polarity and Heterogeneity of Lipid Droplets in Live Cells Using a Push–Pull Fluorophore published pages: 1928-1935, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.8b04218 |
Analytical Chemistry 91/3 | 2020-02-19 |
| 2019 |
Anne Runser, Denis Dujardin, Pauline Ernst, Andrey S. Klymchenko, Andreas Reisch Zwitterionic Stealth Dye-Loaded Polymer Nanoparticles for Intracellular Imaging published pages: 117-125, ISSN: 1944-8244, DOI: 10.1021/acsami.9b15396 |
ACS Applied Materials & Interfaces 12/1 | 2020-02-19 |
| 2018 |
Andreas Reisch, Doriane Heimburger, Pauline Ernst, Anne Runser, Pascal Didier, Denis Dujardin, Andrey S. Klymchenko Proteinâ€Sized Dyeâ€Loaded Polymer Nanoparticles for Free Particle Diffusion in Cytosol published pages: 1805157, ISSN: 1616-301X, DOI: 10.1002/adfm.201805157 |
Advanced Functional Materials 28/48 | 2020-02-19 |
| 2018 |
Bohdan Andreiuk, Andreas Reisch, Eduard Bernhardt, Andrey S. Klymchenko Fighting Aggregationâ€Caused Quenching and Leakage of Dyes in Fluorescent Polymer Nanoparticles: Universal Role of Counterion published pages: 836-846, ISSN: 1861-4728, DOI: 10.1002/asia.201801592 |
Chemistry – An Asian Journal 14/6 | 2020-02-19 |
| 2019 |
Marcelina Cardoso Dos Santos, Anne Runser, Hortense Bartenlian, Aline M. Nonat, Loïc J. Charbonnière, Andrey S. Klymchenko, Niko Hildebrandt, Andreas Reisch Lanthanide-Complex-Loaded Polymer Nanoparticles for Background-Free Single-Particle and Live-Cell Imaging published pages: 4034-4041, ISSN: 0897-4756, DOI: 10.1021/acs.chemmater.9b00576 |
Chemistry of Materials 31/11 | 2020-02-19 |
| 2018 |
Mayeul Collot, Emmanuel Boutant, Maxime Lehmann, Andrey S. Klymchenko BODIPY with Tuned Amphiphilicity as a Fluorogenic Plasma Membrane Probe published pages: 192-199, ISSN: 1043-1802, DOI: 10.1021/acs.bioconjchem.8b00828 |
Bioconjugate Chemistry 30/1 | 2020-02-19 |
| 2019 |
Vitalii Rosiuk, Anne Runser, Andrey Klymchenko, Andreas Reisch Controlling Size and Fluorescence of Dye-Loaded Polymer Nanoparticles through Polymer Design published pages: 7009-7017, ISSN: 0743-7463, DOI: 10.1021/acs.langmuir.9b00721 |
Langmuir 35/21 | 2020-02-19 |
| 2020 |
Mayeul Collot, Emmanuel Boutant, Kyong Tkhe Fam, Lydia DANGLOT, Andrey S. Klymchenko Molecular Tuning of Styryl Dyes Leads to Versatile and Efficient Plasma Membrane Probes for Cell and Tissue Imaging published pages: , ISSN: 1043-1802, DOI: 10.1021/acs.bioconjchem.0c00023 |
Bioconjugate Chemistry | 2020-02-19 |
| 2019 |
Dmytro I. Danylchuk, Seonah Moon, Ke Xu, Andrey S. Klymchenko Switchable Solvatochromic Probes for Live-Cell Super-resolution Imaging of Plasma Membrane Organization published pages: 14920-14924, ISSN: 1433-7851, DOI: 10.1002/anie.201907690 |
Angewandte Chemie International Edition 58/42 | 2020-02-19 |
| 2019 |
Kateryna Trofymchuk, Jurga Valanciunaite, Bohdan Andreiuk, Andreas Reisch, Mayeul Collot, Andrey S. Klymchenko BODIPY-loaded polymer nanoparticles: chemical structure of cargo defines leakage from nanocarrier in living cells published pages: 5199-5210, ISSN: 2050-7518, DOI: 10.1039/c8tb02781a |
Journal of Materials Chemistry B 7/34 | 2020-02-19 |
| 2016 |
Iuliia A. Karpenko, Yosuke Niko, Viktor P. Yakubovskyi, Andriy O. Gerasov, Dominique Bonnet, Yuriy P. Kovtun, Andrey S. Klymchenko Push–pull dioxaborine as fluorescent molecular rotor: far-red fluorogenic probe for ligand–receptor interactions published pages: 3002-3009, ISSN: 2050-7534, DOI: 10.1039/C5TC03411F |
J. Mater. Chem. C 4/14 | 2019-06-06 |
| 2017 |
Fanny Cayre, Simona Mura, Bohdan Andreiuk, Dunja Sobot, Sandrine Gouazou, Didier Desmaële, Andrey S. Klymchenko, Patrick Couvreur In Vivo FRET Imaging to Predict the Risk Associated with Hepatic Accumulation of Squalene-Based Prodrug Nanoparticles published pages: 1700830, ISSN: 2192-2640, DOI: 10.1002/adhm.201700830 |
Advanced Healthcare Materials | 2019-06-06 |
| 2017 |
Kateryna Trofymchuk, Andreas Reisch, Pascal Didier, François Fras, Pierre Gilliot, Yves Mely, Andrey S. Klymchenko Giant light-harvesting nanoantenna for single-molecule detection in ambient light published pages: 657-663, ISSN: 1749-4885, DOI: 10.1038/s41566-017-0001-7 |
Nature Photonics 11/10 | 2019-06-06 |
| 2016 |
Vandamme, Thierry; Klymchenko, Andrey S; Anton, Nicolas; Mercier, Luc; Andreiuk, Bohdan; Goetz, Jacky G; Mély, Yves; Bouchaala, Redouane Integrity of lipid nanocarriers in bloodstream and tumor quantified by near-infrared ratiometric FRET imaging in living mice published pages: 57–67, ISSN: 0168-3659, DOI: 10.1016/j.jconrel.2016.06.027 |
Journal of Controlled Release 236 | 2019-06-06 |
| 2016 |
Ievgen Shulov, Roman V. Rodik, Youri Arntz, Andreas Reisch, Vitaly I. Kalchenko, Andrey S. Klymchenko Protein-Sized Bright Fluorogenic Nanoparticles Based on Cross-Linked Calixarene Micelles with Cyanine Corona published pages: 15884-15888, ISSN: 1433-7851, DOI: 10.1002/anie.201609138 |
Angewandte Chemie International Edition 55/51 | 2019-06-06 |
| 2017 |
Redouane Bouchaala, Nicolas Anton, Halina Anton, Thierry Vandamme, Julien Vermot, Djabi Smail, Yves Mély, Andrey S. Klymchenko Light-triggered release from dye-loaded fluorescent lipid nanocarriers in vitro and in vivo published pages: 414-421, ISSN: 0927-7765, DOI: 10.1016/j.colsurfb.2017.05.035 |
Colloids and Surfaces B: Biointerfaces 156 | 2019-06-06 |
| 2016 |
Yosuke Niko, Pascal Didier, Yves Mely, Gen-ichi Konishi, Andrey S. Klymchenko Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes published pages: 18870, ISSN: 2045-2322, DOI: 10.1038/srep18870 |
Scientific Reports 6/1 | 2019-06-06 |
| 2015 |
Ievgen Shulov, Sule Oncul, Andreas Reisch, Youri Arntz, Mayeul Collot, Yves Mely, Andrey S. Klymchenko Fluorinated counterion-enhanced emission of rhodamine aggregates: ultrabright nanoparticles for bioimaging and light-harvesting published pages: 18198-18210, ISSN: 2040-3364, DOI: 10.1039/C5NR04955E |
Nanoscale 7/43 | 2019-06-06 |
| 2017 |
Klymchenko, Andrey BrightSens - Ultrabright Turn-on Fluorescent Organic Nanoparticles for Amplified Molecular Sensing in Living Cells - H2020 published pages: 78-80, ISSN: 2398-7073, DOI: |
Impact 6/2017 | 2019-06-06 |
| 2016 |
Luca Petrizza, Mayeul Collot, Ludovic Richert, Yves Mely, Luca Prodi, Andrey S. Klymchenko Dye-doped silica nanoparticle probes for fluorescence lifetime imaging of reductive environments in living cells published pages: 104164-104172, ISSN: 2046-2069, DOI: 10.1039/C6RA21427D |
RSC Adv. 6/106 | 2019-06-06 |
| 2017 |
Bohdan Andreiuk, Andreas Reisch, Vasyl G. Pivovarenko, Andrey S. Klymchenko An aluminium-based fluorinated counterion for enhanced encapsulation and emission of dyes in biodegradable polymer nanoparticles published pages: 2309-2316, ISSN: 2052-1537, DOI: 10.1039/c7qm00248c |
Materials Chemistry Frontiers 1/11 | 2019-06-06 |
| 2017 |
Andreas Reisch, Kateryna Trofymchuk, Anne Runser, Guillaume Fleith, Michel Rawiso, Andrey S. Klymchenko Tailoring Fluorescence Brightness and Switching of Nanoparticles through Dye Organization in the Polymer Matrix published pages: 43030-43042, ISSN: 1944-8244, DOI: 10.1021/acsami.7b12292 |
ACS Applied Materials & Interfaces 9/49 | 2019-06-06 |
| 2017 |
Mayeul Collot, Flavien Ponsot, Andrey S. Klymchenko Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore published pages: 6117-6120, ISSN: 1359-7345, DOI: 10.1039/c7cc02418e |
Chemical Communications 53/45 | 2019-06-06 |
| 2017 |
Bohdan Andreiuk, Andreas Reisch, Marion Lindecker, Gautier Follain, Nadine Peyriéras, Jacky G. Goetz, Andrey S. Klymchenko Fluorescent Polymer Nanoparticles for Cell Barcoding In Vitro and In Vivo published pages: 1701582, ISSN: 1613-6810, DOI: 10.1002/smll.201701582 |
Small 13/38 | 2019-06-06 |
| 2016 |
Ievgen Shulov, Youri Arntz, Yves Mély, Vasyl G. Pivovarenko, Andrey S. Klymchenko Non-coordinating anions assemble cyanine amphiphiles into ultra-small fluorescent nanoparticles published pages: 7962-7965, ISSN: 1359-7345, DOI: 10.1039/C6CC03716J |
Chem. Commun. 52/51 | 2019-06-06 |
| 2016 |
Andreas Reisch, Andrey S. Klymchenko Fluorescent Polymer Nanoparticles Based on Dyes: Seeking Brighter Tools for Bioimaging published pages: 1968-1992, ISSN: 1613-6810, DOI: 10.1002/smll.201503396 |
Small 12/15 | 2019-06-06 |
| 2017 |
Andrey S. Klymchenko Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications published pages: 366-375, ISSN: 0001-4842, DOI: 10.1021/acs.accounts.6b00517 |
Accounts of Chemical Research 50/2 | 2019-06-06 |
| 2018 |
Mayeul Collot, Tkhe Kyong Fam, Pichandi Ashokkumar, Orestis Faklaris, Thierry Galli, Lydia Danglot, Andrey S. Klymchenko Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues published pages: 5401-5411, ISSN: 0002-7863, DOI: 10.1021/jacs.7b12817 |
Journal of the American Chemical Society 140/16 | 2019-06-06 |
| 2017 |
Flavien Ponsot, Weida Shen, Pichandi Ashokkumar, Etienne Audinat, Andrey S. Klymchenko, Mayeul Collot PEGylated Red-Emitting Calcium Probe with Improved Sensing Properties for Neuroscience published pages: 1706-1712, ISSN: 2379-3694, DOI: 10.1021/acssensors.7b00665 |
ACS Sensors 2/11 | 2019-06-06 |
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The information about "BRIGHTSENS" are provided by the European Opendata Portal: CORDIS opendata.