PolarNet project deliverables
The page lists 28 deliverables related to the research project "PolarNet".
List of Deliverables
| title and desprition | type | last update |
|---|---|---|
Analysis of Axin1, Axin2, and APC roles in liver and intestinal cell divisionAnalysis of Axin1, Axin2, and APC roles in liver and intestinal cell division involves a) cell-biological analysis of Axin1, Axin2, and APC null phenotypes in liver and intestinal cell division, and b) Mechanistic analysis of links between altered G2/M mitotic programme and increased cell division following Axin loss. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Mechanisms of mechanotransduction during division in epithelial tissueElucidating the mechanisms of mechanotransduction during division in epithelial tissue involves a) integrated understanding of cytokinesis in epithelial tissue, b) uncovering the mechanisms of mechanotrandsuction during division in epithelial tissue, and c) force measurements in whole animal. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Mechanistic role of microtubules in polarization of 3D-cultured epithelial cellsElucidating the mechanistic role of microtubules in polarization of 3D-cultured epithelial cells involves a) quantitative description of the role of microtubule-attachment complexes in cortical polarization in 3D breast epithelia acini and their modification during EMT and invasive growth in 3D, b) characterization of the biochemical connection between microtubule attachment complexes and the Scrib complex, c) quantitative characterization of the role of microtubule dynamics in polarity maintenance in 3D-cultured epithelial cells and the effects of microtubule-targeting agents on this process. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Mechanochemical model of PAR polarity patterning1. Development of a mechanochemical model of PAR polarity patterning will involve a) simultaneous and complete midplane concentration and flowfields of markers of polarity and the actomyosin cortex under wild type and RNAi conditions, b) Identication of which markers of polarity control what activities of the cellular cytoskeleton, c) Development of a quantitative and predictive description of PAR polarity as a process of mechanochemical patterning formation in a regulated thin film active fluid. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Analysis of the mechanisms that maintain tissue integrity in seam epitheliumAnalysis of the mechanisms that maintain tissue integrity in seam epithelium involves a) generation of strains in which apical-basal polarity, cell adhesion, tissue polarity, and various tumour suppressors can be disrupted specifically in the seam epithelium, and b) analysis of genetic interactions between these regulators, with the goal of causing seam cells to leave the epithelium. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Biochemical characterization of the Scrib polarity complexBiochemical characterization of the Scrib polarity complex will involve a) identification of new partners of the mammalian polarity protein Scrib, b) biochemical characterization of the Scrib polarity complex in migrating cells and polarized epithelial cells, and c) characterization of the polarity function of Scrib partners in migrating cells and polarized epithelial cells. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
GTPase-regulator circuits essential for yeast and C. elegans polarityElucidation of GTPase-regulator circuits essential for yeast and C. elegans polarity will involve a) identification of GTPase-regulator circuits essential for polarized growth in yeast and polarity establishment and maintenance in C. elegans early embryos, b) characterization of the role of Arf/Rab GTPases in epithelial polarity and tissue organization, and c) generation of a number of C. elegans strains expressing GFP or mCherry tagged Arf and Rab GTPases and their effectors, and generation of specific point mutations therein using the CRISPR-Cas9 system. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Computational model of epithelial regeneration after woundingDeveloping a computational model of epithelial regeneration after wounding involves a) live imaging of Drosophila wing disc regeneration and polarity re-establishment, b) quantification of changes in tissue mechanics after the wound and during the regeneration process, and c) building of a computational model of epithelial regeneration. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Analysis of cell contact dependent transmission of Wnt-signal in seam cell PCPAnalysis of cell contact dependent transmission of Wnt-signal in seam cell PCP involves a) C. elegans clonal analysis of lineage-specific knock out mutants, b) analysis of cell-cell contact dependent transmission of the Wnt-signal in seam cell PCP, and c) identification of components critical for mitotic spindle and cleavage plane positioning. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Review article by the ESRs incorporating data obtained by PolarNetReview article by the ESRs incorporating data obtained by PolarNet Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Summaries of ESRs results placed on website by the ESRsSummaries of ESRs results placed on website by the ESRs Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Websites, patent fillings, videos etc. | 2020-03-11 |
Comprehensive, quantitative mathematical description of PAR polarityDeveloping a comprehensive, quantitative mathematical description of PAR polarity will involve a) Defining mobility parameters for PAR network components, including diffusion coefficients and membrane association/dissociation rates, b) Defining binding parameters governing pairwise interactions between PAR com-ponents. c) Construction and analysis of a model of PAR polarity based on parameters in (a) and (b) to identify core design principles of PAR network. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Characterization of PAR, Crumbs and Scribble in C. elegans larval epitheliaCharacterization of PAR, Crumbs and Scribble in C. elegans larval epithelia will involve a) generation of genetic null alleles of members of the Par, Crumbs, and Scribble complexes for which such alleles do not exist yet, and b) analysis of inactivation of members of these complexes on polarity in the intestine and seam cells. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Semi-automated culturing of organoids in a bioreactorSemi-automated culturing of organoids in a bioreactor involves a) determining the correlation between processing conditions and organoid morphology and function, and b) bespoke hollow fibre bioreactor design for processing of organoids. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Video highlighting importance of polarity for website & youtubeVideo highlighting importance of polarity for website & youtube Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Websites, patent fillings, videos etc. | 2020-03-11 |
Super-resolution localization of polarity regulators at the plasma membraneSuper-resolution localization of polarity regulators at the plasma membrane will involve a) immunization of llama\'s with MDCK cells to generate a MDCK-immune library, b) production and purification of the membrane anchoring component for selection of specific nanobodies, c) selection and characterization of nanobodies binding to anchoring proteins, d) imaging of membrane anchoring of the spindle in 3D by super-resolution microscopy of 2D MDCK cell cultures, and e) quantitative characterization of the organization of the spindle orientation by anchoring complexes and the effect of EGF thereon. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Symposium 2Network Symposium Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Other | 2020-03-11 |
Quantitative analyses of local polarity perturbations on polarized growthQuantitative analyses of local polarity perturbations on polarized growth will involve a) generation of strains which can generate locally increased concentrations of PS, PIP2, activated Cdc42 and activated Rho1, b) Quantitative analyses of local perturbation of PS, PIP2, activated Cdc42 and activated Rho1 levels on external mediated polarized growth and force generation. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Role of phosphoinositides in control of cell polarity during collective migrationCharacterizing the role of phosphoinositides in control of cell polarity during collective migration will involve a) characterization of phosphoinositide distribution during collective migration, b) characterisation of the role of phosphoinositides and more precisely of the lipid phosphatase PTEN in the control of cell polarity during collective migration, c) identification of signalling cascades controlled downstream of phosphoinositide signalling, and d) elucidation of the consequences of PTEN loss of functions in tumor spreading. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2020-03-11 |
Bi-weekly ‘Polarizing Cells’ blog entry on websiteBi-weekly ‘Polarizing Cells’ blog entry on website Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Websites, patent fillings, videos etc. | 2020-03-11 |
Symposium 3Closing symposium Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Other | 2020-03-11 |
ESRs evaluated for Doctorate degree applicationAll ESR are evaluated by the Supervisory Board for eligibility to apply for a Doctorate degree. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Other | 2020-03-11 |
Draft Periodic Reportcompulsory deliverable Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2019-06-19 |
Symposium 1Network symposium Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Other | 2019-06-19 |
Midterm review Meetingcompulsory deliverable Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Other | 2019-06-19 |
Summer SchoolNetwork Summer School Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Other | 2019-06-19 |
Progress Reportcompulsory deliverable Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Documents, reports | 2019-06-19 |
Web presenceWeb presence established. Programme: H2020-EU.1.3.1. - Topic(s): MSCA-ITN-2015-ETN |
Websites, patent fillings, videos etc. | 2019-06-19 |