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Lipid and Polarity SIGNED

The diffusion and nanoclustering of a polarity module in the lipid environment

Total Cost €

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EC-Contrib. €

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Partnership

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 Lipid and Polarity project word cloud

Explore the words cloud of the Lipid and Polarity project. It provides you a very rough idea of what is the project "Lipid and Polarity" about.

regulation    proteins    mammalian    gef    small    cancer    psd2    play    quantification    vesicle    pivotal    completely    contribution    nanoclusters    pe    shown    i4    leaflet    gtpase    dramatic    trafficking    universal    cho1    questions    yeast    resolution    mechanisms    imaging    phosphatidylserine    environment    dynamics    function    though    spatial    cap    asymmetric    plasma    bem1    budding    interact    polar    molecules    ras    lipids    p2    super    maintenance    entails    regulator    clustering    biology    vivo    cerevisiae    analyze    assembly    cells    individual    localizes    demonstrated    localization    family    signaling    cdc42    components    eukaryotic    asymmetrically    diffusion    ps    saccharomyces    rho    internal    protein    cdc24    molecule    pole    orientation    cell    embryogenesis    powerful    mutants    organization    membrane    scaffold    pm    pi4p    tracking    single    nano    imparts    module    psd1    polarity    phospholipids    activating    forms    establishment    membranes    influences    central    lipid    diseases    cellular    live   

Project "Lipid and Polarity" data sheet

The following table provides information about the project.

Coordinator
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS 

Organization address
address: RUE MICHEL ANGE 3
city: PARIS
postcode: 75794
website: www.cnrs.fr

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country France [FR]
 Project website http://ibgc.cnrs.fr/
 Total cost 185˙076 €
 EC max contribution 185˙076 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2015
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2016
 Duration (year-month-day) from 2016-04-01   to  2018-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS FR (PARIS) coordinator 185˙076.00

Map

 Project objective

Cellular polarity is a universal feature of eukaryotic biology. Failure to control cell orientation has dramatic consequences on embryogenesis and diseases including cancer. The establishment of cellular polarity often entails the asymmetric distribution of plasma membrane (PM) lipids signaling proteins and vesicle trafficking. The function of Rho proteins in cell polarity has been demonstrated from mammalian cells to yeast. The small GTPase Cdc42 is a pivotal regulator of polarity establishment and maintenance. A central feature of Cdc42 function is its ability to interact with membrane. In budding yeast, Cdc42 localizes asymmetrically at the PM, at the pole of the cell, along with the scaffold protein Bem1 and the Cdc42 activating GEF Cdc24. The asymmetric distribution of lipids in the internal leaflet of the membrane play a crucial role in the regulation of Cdc42 activity and localization, though the mechanisms are not completely understood. The organization of lipids in the PM influences the spatial distribution and activity of key signaling proteins as Ras family proteins. Ras forms nanoclusters in cell membranes that are essential for signaling and Phosphatidylserine lipids have been shown to play a role in the assembly of K-Ras nanoclusters in mammalian cells. In this work, we will study Cdc42, Cdc24 and Bem1 dynamics in the membrane and particularly at the polar cap. To address these challenging questions, we will be using lipid mutants (cho1∆, psd1∆ psd2∆…) in Saccharomyces cerevisiae. We are tracking Cdc42 polarity module components in these mutants at the single molecule level by super-resolution imaging in live cells. This system enables the quantification of the diffusion and clustering of individual molecules at the PM, and the contribution that the lipid environment imparts in vivo. In addition, using this powerful system, we will analyze the nano-organization of different phospholipids involved in signaling (PS, PE, P(I4,5)P2, PI4P).

 Publications

year authors and title journal last update
List of publications.
2018 Elodie Sartorel, Caner Ünlü, Mini Jose, Aurélie Massoni-Laporte, Julien Meca, Jean-Baptiste Sibarita, Derek McCusker
Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion
published pages: mbc.E18-01-0051, ISSN: 1059-1524, DOI: 10.1091/mbc.E18-01-0051
Molecular Biology of the Cell 2019-06-13

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