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GutBCells SIGNED

Cellular Dynamics of Intestinal Antibody-Mediated Immune Response

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

GutBCells project word cloud

Explore the words cloud of the GutBCells project. It provides you a very rough idea of what is the project "GutBCells" about.

expression layers recombination lymph commensal lymphoid gut sorting microbes germinal techniques permanently implementing periods gene class oral immunological induction vaccinations gc tracking plan fluorescent place tissues signaling memory cells implicate vaccine plasma prevent lasting elicited molecular mucosa peripheral diseases regimen prolong analyze intraceullar nodes simplest interactions fate adjust regulation galt mice prolonged dynamics antibody tracing labeling intravital examine takes peyer vivo purpose intestinal daughter goals iga injected imaging switch extend perform components cellular compartment associate mucosal migration enter vaccination center track immunization reporter inducing immunizations photoactivation time administration compartments immune patches mediated human cell

Project "GutBCells" data sheet

The following table provides information about the project.

Coordinator	WEIZMANN INSTITUTE OF SCIENCE Organization address address: HERZL STREET 234 city: REHOVOT postcode: 7610001 website: www.weizmann.ac.il contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Israel [IL]
Total cost	1˙375˙000 €
EC max contribution	1˙375˙000 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2015-STG
Funding Scheme	ERC-STG
Starting year	2016
Duration (year-month-day)	from 2016-05-01 to 2021-04-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	WEIZMANN INSTITUTE OF SCIENCE WEIZMANN INSTITUTE OF SCIENCE Organization address address: HERZL STREET 234 city: REHOVOT postcode: 7610001 website: www.weizmann.ac.il contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	IL (REHOVOT)	coordinator	1˙375˙000.00

Map

Project objective

Vaccination is widely used to prevent human diseases by inducing the formation of cellular and antibody-mediated immune responses for induction of long lasting immunological memory. Although most studies focus on immune responses elicited against injected immunizations, the simplest delivery of a vaccine regimen is by oral administration. The cellular and molecular components of the antibody immune response in peripheral lymph nodes in response to immunization are well described, however, much less is known about the dynamics of immune cells in gut associate lymphoid tissues (GALT) and adjust intestinal mucosal tissues. In the proposed research plan I will implicate intravital in vivo imaging for analysis of the cellular component of the antibody immune response in intestinal tissues. My goals are: 1. To track germinal center (GC) T cells for prolong time periods in peripheral lymph nodes and GALT and determine if they enter the memory compartment. For this purpose I will develop a new photoactivation method for permanently labeling immune cells and fate tracing of their daughter cells. 2. To examine T-B interactions and their regulation by intraceullar signaling pathways in GALT and to determine where and when class switch recombination to IgA takes place. For this purpose I will use intravital imaging of fluorescent reporter mice. 3. I will analyze the dynamics of plasma cell migration from Peyer’s patches to the mucosa by implementing state of the art photoactivation and imaging techniques that allow prolonged cell tracking. I will also use photoactivation approaches for sorting plasma cells from specific intestinal layers and perform gene expression analysis. 4. I will develop a new method to study dynamics and fate of B cells specific for commensal microbes in the GC, memory and plasma cell compartments. This research plan will extend our knowledge of the antibody immune response in intestinal tissues towards the future design of improved oral vaccinations.

Publications

List of publications.
year	authors and title	journal	last update
2017	Chiara Medaglia, Amir Giladi, Liat Stoler-Barak, Marco De Giovanni, Tomer Meir Salame, Adi Biram, Eyal David, Hanjie Li, Matteo Iannacone, Ziv Shulman, Ido Amit Spatial reconstruction of immune niches by combining photoactivatable reporters and scRNA-seq published pages: 1622-1626, ISSN: 0036-8075, DOI: 10.1126/science.aao4277	Science 358/6370	2019-06-19
2017	Irina Zaretsky, Ofir Atrakchi, Roei D. Mazor, Liat Stoler-Barak, Adi Biram, Sara W. Feigelson, Alexander D. Gitlin, Britta Engelhardt, Ziv Shulman ICAMs support B cell interactions with T follicular helper cells and promote clonal selection published pages: jem.20171129, ISSN: 0022-1007, DOI: 10.1084/jem.20171129	The Journal of Experimental Medicine	2019-06-19

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The information about "GUTBCELLS" are provided by the European Opendata Portal: CORDIS opendata.