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MuCellWall

Seed mucilage envelope as a new structural model in cell wall research

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

MuCellWall project word cloud

Explore the words cloud of the MuCellWall project. It provides you a very rough idea of what is the project "MuCellWall" about.

treatment lepidium fraction glycoproteins creation mscs techniques material special location spatial cells examined host fibrillar sativum gold msc restricted plant secreting combinations incomplete genetic realistic representative interdisciplinary wild dynamic structure despite kiel place precise visualization extracellular direct cell usually arranged laboratory mostly hemicelluloses attached opening divided visualize lcsm sem exactly mucilage antibodies models network loose components organization chemicals destroy overcome quite mutants give invasive particles model enzymes pectins polysaccharides complexes leucodens extensive modern possibilities arabidopsis university seeds artemisia microstructural created detect excellent microscopes structural cellulosic divers biochemical matrix thaliana wall

Project "MuCellWall" data sheet

The following table provides information about the project.

Coordinator	CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL Organization address address: OLSHAUSENSTRASSE 40 city: KIEL postcode: 24118 website: http://www.uni-kiel.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Total cost	171˙460 €
EC max contribution	171˙460 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2015
Funding Scheme	MSCA-IF-EF-ST
Starting year	2016
Duration (year-month-day)	from 2016-09-01 to 2018-08-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL CHRISTIAN-ALBRECHTS-UNIVERSITAET ZU KIEL Organization address address: OLSHAUSENSTRASSE 40 city: KIEL postcode: 24118 website: http://www.uni-kiel.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (KIEL)	coordinator	171˙460.00

Map

Project objective

The plant cell wall is dynamic, complexes structure composed of polysaccharides divided into fibrillar (cellulosic) fraction and extracellular matrix (hemicelluloses and pectins). All the components are arranged in a network structure characteristic of the cell wall. The proposed, general spatial models of the cell wall based mostly on various biochemical and/or microstructural analysis, which have usually been invasive by treatment with enzymes or chemicals, which can destroy the cell wall organization and allow to visualize a selected components. Despite of extensive research our knowledge about the cell wall is still incomplete and restricted mostly to the biochemical or genetic studies. The main aim of this project will be the determination of the cell wall components i.e. polysaccharides, glycoproteins, and the determination their spatial distribution in a special type of cell wall of the mucilage secreting cells (MSCs). The obtained results will allow creation a direct spatial cell wall model. The used material e.g. seeds of Arabidopsis thaliana mutants and wild type, Artemisia leucodens or Lepidium sativum will be an excellent and so far not examined study model due to characteristic loose spatial structure of the MSC cell wall. The use of modern techniques (in a quite new combinations) e.g. antibodies with attached gold particles and precise visualization in divers microscopes (e.g. SEM, LCSM) available at the host laboratory (Kiel University) will allow to overcome invasive procedures and let to detect the exactly location of the components. The realistic, representative cell wall spatial model will be created allowing for better understanding the different processes taking place within the cell wall. The project will be implemented at an interdisciplinary level opening a new possibilities for various cell wall studies. The project will give a new insight into the cell wall structural studies and lead to significant advances in this field.

Publications

List of publications.
year	authors and title	journal	last update
2017	Agnieszka Kreitschitz, Stanislav N. Gorb How does the cell wall â€˜stickâ€™ in the mucilage? A detailed microstructural analysis of the seed coat mucilaginous cell wall published pages: 9-22, ISSN: 0367-2530, DOI: 10.1016/j.flora.2017.02.010	Flora 229	2019-04-14

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The information about "MUCELLWALL" are provided by the European Opendata Portal: CORDIS opendata.