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MERA SIGNED

Mechanism of Enzyme Rhodopsin Activation

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

MERA project word cloud

Explore the words cloud of the MERA project. It provides you a very rough idea of what is the project "MERA" about.

mechanism dynamics crystals fungus neuronal totally photoreceptor laboratory regulation lines understand uncharacterized ensemble phosphodiesterase though first ion epr subdivided ray photoreceptors awaits computer model time urgently cyclase uv channelrhodopsin cgmp variety obtain camp neuroscience dimensional engineering cell rhodopsin channel moderate optogenetics characterization flashes resolved rhpde final enzyme elegant class emersonii biophysical messenger accordingly revolutionized decade activation generation technologies perspectives inactivation gated fourth vis cells intensities activate mera neurons family crystallography sensory coexpression optogenetic offers ftir rhgc outcome desirable conversion hypopolarizer guanylyl broad light aided raman blastocladiella spectroscopy discovered ultimate solution

Project "MERA" data sheet

The following table provides information about the project.

Coordinator	HUMBOLDT-UNIVERSITAET ZU BERLIN Organization address address: UNTER DEN LINDEN 6 city: BERLIN postcode: 10117 website: www.hu-berlin.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Germany [DE]
Total cost	2˙398˙750 €
EC max contribution	2˙398˙750 € (100%)
Programme	1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
Code Call	ERC-2015-AdG
Funding Scheme	ERC-ADG
Starting year	2016
Duration (year-month-day)	from 2016-10-01 to 2021-09-30

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	HUMBOLDT-UNIVERSITAET ZU BERLIN HUMBOLDT-UNIVERSITAET ZU BERLIN Organization address address: UNTER DEN LINDEN 6 city: BERLIN postcode: 10117 website: www.hu-berlin.de contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	DE (BERLIN)	coordinator	2˙398˙750.00

Map

Project objective

'Channelrhodopsin, which was discovered and described as a light-gated ion channel in my laboratory, has revolutionized the field of neuroscience over the past decade by enabling researchers to specifically activate selected neurons in a large ensemble of neuronal cells with short light flashes, a technology we now call 'Optogenetics.' However, though highly desirable, the inactivation of specific cells using moderate or low light intensities is not yet possible. The recently discovered rhodopsin-guanylyl-cyclase (RhGC) of the fungus Blastocladiella emersonii offers an elegant solution to this problem. Moreover, RhGC is a totally novel and uncharacterized sensory photoreceptor, and the first member of an enzyme rhodopsin family that urgently awaits in-depth characterization. Accordingly, the goal of the “mechanism of enzyme rhodopsin activation” (MERA) proposal is to obtain a comprehensive understanding of this novel photoreceptor, and to determine its functionality for broad application in optogenetics and other research fields. The MERA project is subdivided into four objectives. The first objective is the characterization and engineering of RhGC in cell lines and neurons as well as coexpression of RhGC with a cGMP-gated K channel to develop a 'Light-Hypopolarizer' for cell inactivation. The second objective is to understand the dynamics of RhGC using a variety of biophysical technologies including time resolved UV-vis, FTIR, and Raman and EPR spectroscopy. A third objective is the generation of crystals for X-ray crystallography and the development of a three dimensional RhGC model. The fourth and final objective is the computer-aided conversion of RhGC into a rhodopsin-phosphodiesterase (RhPDE) for down-regulation of the second messenger cGMP and/or cAMP using light. The ultimate outcome will be a detailed understanding of a novel class of sensory photoreceptors with new perspectives for broad optogenetic applications.'

Publications

List of publications.
year	authors and title	journal	last update
2018	Ulrike Scheib, Matthias Broser, Oana M. Constantin, Shang Yang, Shiqiang Gao, Shatanik Mukherjee, Katja Stehfest, Georg Nagel, Christine E. Gee, Peter Hegemann Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3â€‰Ã… structure of the adenylyl cyclase domain published pages: , ISSN: 2041-1723, DOI: 10.1038/s41467-018-04428-w	Nature Communications 9/1	2019-06-13
2017	Alfons Penzkofer, Ulrike Scheib, Katja Stehfest, Peter Hegemann Absorption and Emission Spectroscopic Investigation of Thermal Dynamics and Photo-Dynamics of the Rhodopsin Domain of the Rhodopsin-Guanylyl Cyclase from the Nematophagous Fungus Catenaria anguillulae published pages: 2099, ISSN: 1422-0067, DOI: 10.3390/ijms18102099	International Journal of Molecular Sciences 18/10	2019-06-13
2019	Scheib, U. Biochemische und biophysikalische Charakterisierung von Rhodopsin-Guanylylzyklasen. published pages: , ISSN: , DOI:		2019-06-06
2019	Shatanik Mukherjee, Peter Hegemann, Matthias Broser Enzymerhodopsins: novel photoregulated catalysts for optogenetics published pages: 118-126, ISSN: 0959-440X, DOI: 10.1016/j.sbi.2019.02.003	Current Opinion in Structural Biology 57	2019-06-06

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