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ACtIVAtE SIGNED

The virulence potential of human pathogens: how Acinetobacter baumannii survives Acanthamoeba castellanii predation

Total Cost €

EC-Contrib. €

Partnership

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Outcomes and
results

ACtIVAtE project word cloud

Explore the words cloud of the ACtIVAtE project. It provides you a very rough idea of what is the project "ACtIVAtE" about.

viability context pathogen nosocomial symbiotic broadly monitoring light complemented manners mechanistic superbug resistance individual host trophozoite bacterial amoebal successful interactions screening unknown fluorescence virulence levels isolates acanthamoeba qualitative cyst electron predation complementary seq human original biphasic screen tested tn cell followed reporter clinical understand bacteria despite health time throughput poorly shed mutant lacking acinetobacter interaction assays correlated outcome localization amoeba grazing cellular live imaging amoebae strains stages pathogenicity induction attenuated validated global threat precise castellanii single infection relevance pathogens population quantitative microscopy generating techniques deadly model mechanisms experimental baumannii decipher

Project "ACtIVAtE" data sheet

The following table provides information about the project.

Coordinator	UNIVERSITE DE NAMUR ASBL Organization address address: RUE DE BRUXELLES 61 city: NAMUR postcode: 5000 website: www.unamur.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.
Coordinator Country	Belgium [BE]
Total cost	172˙800 €
EC max contribution	172˙800 € (100%)
Programme	1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
Code Call	H2020-MSCA-IF-2016
Funding Scheme	MSCA-IF-EF-ST
Starting year	2018
Duration (year-month-day)	from 2018-01-01 to 2019-12-31

Partnership

Take a look of project's partnership.

#	participants	country	role	EC contrib. [€]
1	UNIVERSITE DE NAMUR ASBL UNIVERSITE DE NAMUR ASBL Organization address address: RUE DE BRUXELLES 61 city: NAMUR postcode: 5000 website: www.unamur.be contact info title: n.a. name: n.a. surname: n.a. function: n.a. email: n.a. telephone: n.a. fax: n.a.	BE (NAMUR)	coordinator	172˙800.00

Map

Project objective

The human pathogen Acinetobacter baumannii represents a deadly threat to human health. Despite its established clinical relevance, the pathogenicity of this nosocomial superbug is poorly understood. The biphasic amoeba Acanthamoeba castellanii is a cellular infection model broadly used to decipher the virulence mechanisms of human pathogens, as bacterial resistance upon amoebal predation (grazing) are often correlated with their pathogenicity potential in human. However, high-throughput screening approaches to investigate the virulence mechanisms of A. baumannii using this amoeba in an infection context are still lacking. This project aims at applying original large-scale screening approaches to identify A. baumannii virulence factors using the amoeba A. castellanii as host-pathogen system. The proposed experimental set up allows monitoring the outcome of the bacteria-amoebae interactions in qualitative and quantitative manners, at both population and single cell levels. Virulence induction of A. baumannii, as well as the use of successful clinical isolates will be tested using several complementary grazing assays. Precise localization of A. baumannii reporter strains will be done on both trophozoite and cyst stages of A. castellanii, complemented with (i) real-time and live-cell fluorescence imaging as well as (ii) electron microscopy techniques. Viability assays for both bacteria and amoebae will be done to understand the symbiotic outcome of this interaction. The global resistance potential of A. baumannii will be assessed by (i) Tn-seq analysis and (ii) high-throughput screen of individual mutant strains, followed by in depth mechanistic studies on validated attenuated strains. This project will shed light on the yet unknown global virulence mechanisms of A. baumannii by generating original screening methods using A. castellanii as an infection cellular model.

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The information about "ACTIVATE" are provided by the European Opendata Portal: CORDIS opendata.