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ACtIVAtE SIGNED

The virulence potential of human pathogens: how Acinetobacter baumannii survives Acanthamoeba castellanii predation

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 ACtIVAtE project word cloud

Explore the words cloud of the ACtIVAtE project. It provides you a very rough idea of what is the project "ACtIVAtE" about.

validated    mutant    global    individual    complementary    viability    throughput    biphasic    interaction    castellanii    isolates    monitoring    tested    quantitative    outcome    model    induction    poorly    acinetobacter    cyst    levels    mechanistic    decipher    light    mechanisms    understand    bacteria    interactions    context    relevance    amoebae    single    symbiotic    followed    bacterial    broadly    screen    acanthamoeba    cell    unknown    human    threat    stages    correlated    deadly    techniques    live    qualitative    experimental    time    infection    lacking    generating    attenuated    virulence    electron    precise    superbug    baumannii    health    reporter    amoeba    amoebal    resistance    seq    despite    predation    manners    nosocomial    trophozoite    strains    pathogen    original    screening    pathogens    cellular    shed    successful    tn    pathogenicity    fluorescence    microscopy    imaging    complemented    grazing    clinical    population    localization    assays    host   

Project "ACtIVAtE" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE NAMUR ASBL 

Organization address
address: RUE DE BRUXELLES 61
city: NAMUR
postcode: 5000
website: www.unamur.be

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Belgium [BE]
 Total cost 172˙800 €
 EC max contribution 172˙800 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2018
 Duration (year-month-day) from 2018-01-01   to  2019-12-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE NAMUR ASBL BE (NAMUR) coordinator 172˙800.00

Map

 Project objective

The human pathogen Acinetobacter baumannii represents a deadly threat to human health. Despite its established clinical relevance, the pathogenicity of this nosocomial superbug is poorly understood. The biphasic amoeba Acanthamoeba castellanii is a cellular infection model broadly used to decipher the virulence mechanisms of human pathogens, as bacterial resistance upon amoebal predation (grazing) are often correlated with their pathogenicity potential in human. However, high-throughput screening approaches to investigate the virulence mechanisms of A. baumannii using this amoeba in an infection context are still lacking. This project aims at applying original large-scale screening approaches to identify A. baumannii virulence factors using the amoeba A. castellanii as host-pathogen system. The proposed experimental set up allows monitoring the outcome of the bacteria-amoebae interactions in qualitative and quantitative manners, at both population and single cell levels. Virulence induction of A. baumannii, as well as the use of successful clinical isolates will be tested using several complementary grazing assays. Precise localization of A. baumannii reporter strains will be done on both trophozoite and cyst stages of A. castellanii, complemented with (i) real-time and live-cell fluorescence imaging as well as (ii) electron microscopy techniques. Viability assays for both bacteria and amoebae will be done to understand the symbiotic outcome of this interaction. The global resistance potential of A. baumannii will be assessed by (i) Tn-seq analysis and (ii) high-throughput screen of individual mutant strains, followed by in depth mechanistic studies on validated attenuated strains. This project will shed light on the yet unknown global virulence mechanisms of A. baumannii by generating original screening methods using A. castellanii as an infection cellular model.

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The information about "ACTIVATE" are provided by the European Opendata Portal: CORDIS opendata.

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