Opendata, web and dolomites

NEURO_NMD SIGNED

Functional impact of alternative splicing coupled to nonsense-mediated decay in developing neurons

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 NEURO_NMD project word cloud

Explore the words cloud of the NEURO_NMD project. It provides you a very rough idea of what is the project "NEURO_NMD" about.

contributes    post    appropriate    insights    questions    genetic    brain    modulating    manner    global    fashion    gene    neuronal    neural    elucidate    embryonic    transcriptome    undergoing    precise    considerable    time    corresponding    ptbp1    orchestrate    uncover    genes    exons    undergoes    mature    inclusion    morphological    antisense    encoding    significance    primary    coupled    coordinated    regulation    quantitative    decay    underlying    stages    mechanism    seq    nonsense    spatiotemporal    mediated    unclear    resolved    stem    splicing    inhibiting    actin    mechanisms    separate    relies    inactivation    mrna    network    alternative    mouse    poorly    neurons    first    contexts    cell    fit    repertoire    sequencing    differentiation    critical    earlier    functional    biological    systematically    cytoskeleton    cells    developmental    acutely    expression    precursor    regulator    attain    dynamics    oligonucleotides    transcriptional    functions    rna    nmd   

Project "NEURO_NMD" data sheet

The following table provides information about the project.

Coordinator
KING'S COLLEGE LONDON 

Organization address
address: STRAND
city: LONDON
postcode: WC2R 2LS
website: www.kcl.ac.uk

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 183˙454 €
 EC max contribution 183˙454 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2016
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2017
 Duration (year-month-day) from 2017-05-01   to  2019-12-04

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    KING'S COLLEGE LONDON UK (LONDON) coordinator 183˙454.00

Map

 Project objective

Differentiation of precursor cells into mature neurons relies on transcriptome-wide changes in gene expression that have to be coordinated in a precise spatiotemporal fashion. Alternative pre-mRNA splicing coupled to nonsense-mediated decay (AS-NMD) is a widespread post-transcriptional mechanism known to orchestrate gene expression dynamics in developmental contexts. Earlier studies identified several neural targets of this pathway; however, in most cases, the extent to which AS-NMD contributes to the overall gene expression dynamics and biological significance of this regulation is poorly understood. Moreover, whether AS-NMD target repertoire undergoes considerable changes in developing brain and how this might fit to the global regulation network underlying neuronal differentiation remains unclear. I will address these questions using two separate approaches. First, I will investigate novel AS-NMD targets encoding actin cytoskeleton factors and controlled by an important regulator of neuronal alternative splicing, Ptbp1. I will elucidate the extent of AS-NMD regulation in these genes by modulating the inclusion of the NMD-promoting exons with corresponding antisense oligonucleotides. in mouse embryonic stem cells undergoing neuronal differentiation, neural stem cells and primary neurons. Second, I will systematically analyse how NMD contributes to different stages of neuronal development by acutely inhibiting this pathway in a time-resolved manner using genetic means. I will then identify gene expression effects and functional consequences of NMD inactivation using transcriptome sequencing (RNA-Seq) and appropriate cell biological methods. All in all, this work will provide critical quantitative insights into AS-NMD functions and uncover novel mechanisms allowing neurons to attain their unique morphological and functional properties.

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "NEURO_NMD" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "NEURO_NMD" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.3.2.)

MarshFlux (2020)

The effect of future global climate and land-use change on greenhouse gas fluxes and microbial processes in salt marshes

Read More  

TheaTheor (2018)

Theorizing the Production of 'Comedia Nueva': The Process of Play Configuration in Spanish Golden Age Theater

Read More  

eXcape3D (2019)

Functional dissection of X-linked regulatory DNA: unravelling the impact of genome topology on transcriptional regulation

Read More