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3DCellPhase- SIGNED

In situ Structural Analysis of Molecular Crowding and Phase Separation

Total Cost €

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EC-Contrib. €

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Partnership

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 3DCellPhase- project word cloud

Explore the words cloud of the 3DCellPhase- project. It provides you a very rough idea of what is the project "3DCellPhase-" about.

thinning    cytoplasmic    tomography    beam    form    examination    nucleation    microscopy    principles    direct    insights    quantitative    basis    cell    biochemical    liquids    microtubule    cellular    readout    separation    assemblies    organize    lack    suitable    enclosed    living    organization    stress    nature    variations    eukaryotic    amorphous    liquid    description    dynamic    structural    shows    crowded    dynamically    techniques    gels    transparent    local    functionally    compartments    environment    progress    separated    membrane    reconstituting    exhibit    assembly    assemble    context    event    cells    volume    correlative    elucidate    despite    combine    windows    question    illusive    bodies    brings    molecular    fundamental    reactions    vitro    electron    rna    cytoplasm    site    biology    cryo    prepare    sites    preparation    ion    fibers    3d    fluorescence    centrosomes    eukaryotes    intriguingly    triggered    excluded    driving    guided    granules    vivo   

Project "3DCellPhase-" data sheet

The following table provides information about the project.

Coordinator
EUROPEAN MOLECULAR BIOLOGY LABORATORY 

Organization address
address: Meyerhofstrasse 1
city: HEIDELBERG
postcode: 69117
website: http://www.embl.de

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Germany [DE]
 Total cost 1˙228˙125 €
 EC max contribution 1˙228˙125 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-STG
 Funding Scheme ERC-STG
 Starting year 2018
 Duration (year-month-day) from 2018-02-01   to  2023-01-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    EUROPEAN MOLECULAR BIOLOGY LABORATORY DE (HEIDELBERG) coordinator 1˙228˙125.00

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 Project objective

This proposal brings together two fields in biology, namely the emerging field of phase-separated assemblies in cell biology and state-of-the-art cellular cryo-electron tomography, to advance our understanding on a fundamental, yet illusive, question: the molecular organization of the cytoplasm.

Eukaryotes organize their biochemical reactions into functionally distinct compartments. Intriguingly, many, if not most, cellular compartments are not membrane enclosed. Rather, they assemble dynamically by phase separation, typically triggered upon a specific event. Despite significant progress on reconstituting such liquid-like assemblies in vitro, we lack information as to whether these compartments in vivo are indeed amorphous liquids, or whether they exhibit structural features such as gels or fibers. My recent work on sample preparation of cells for cryo-electron tomography, including cryo-focused ion beam thinning, guided by 3D correlative fluorescence microscopy, shows that we can now prepare site-specific ‘electron-transparent windows’ in suitable eukaryotic systems, which allow direct examination of structural features of cellular compartments in their cellular context. Here, we will use these techniques to elucidate the structural principles and cytoplasmic environment driving the dynamic assembly of two phase-separated compartments: Stress granules, which are RNA bodies that form rapidly in the cytoplasm upon cellular stress, and centrosomes, which are sites of microtubule nucleation. We will combine these studies with a quantitative description of the crowded nature of cytoplasm and of its local variations, to provide a direct readout of the impact of excluded volume on molecular assembly in living cells. Taken together, these studies will provide fundamental insights into the structural basis by which cells form biochemical compartments.

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The information about "3DCELLPHASE-" are provided by the European Opendata Portal: CORDIS opendata.

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