Opendata, web and dolomites

PneumoCaTChER SIGNED

The role of cell-to-cell variability in pneumococcal virulence and antibiotic resistance

Total Cost €

0

EC-Contrib. €

0

Partnership

0

Views

0

 PneumoCaTChER project word cloud

Explore the words cloud of the PneumoCaTChER project. It provides you a very rough idea of what is the project "PneumoCaTChER" about.

diverse    places    vice    unclear    collaborations    engineered    hypothesis    transcriptomics    opportunistic    elude    screening    bacteria    pathogenic    driving    grow    excellent    epithelial    originate    perform    pathogens    models    therapy    pneumoniae    cycle    helps    pathogen    combined    strategies    infections    imaging    biology    within    environment    antibiotic    overview    cellular    heterogeneity    influences    cells    cell    innovative    reveal    single    immune    dual    host    rates    poorly    platforms    insights    synthetic    pneumococcal    bistable    sources    gene    crispri    treatment    microbe    engineering    microfluidics    phenotype    variation    rna    lung    expression    though    noise    bacterial    human    combining    position    switches    outcome    underlying    pressure    resistance    setup    genetic    exhibit    populations    pneumococci    quantitative    exploited    versa    variability    streptococcus    resist    infection    virulence    mechanisms    molecular    clonal    phenotypic    seq   

Project "PneumoCaTChER" data sheet

The following table provides information about the project.

Coordinator
UNIVERSITE DE LAUSANNE 

Organization address
address: Quartier Unil-Centre Bâtiment Unicentre
city: LAUSANNE
postcode: 1015
website: www.unil.ch

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country Switzerland [CH]
 Total cost 1˙999˙735 €
 EC max contribution 1˙999˙735 € (100%)
 Programme 1. H2020-EU.1.1. (EXCELLENT SCIENCE - European Research Council (ERC))
 Code Call ERC-2017-COG
 Funding Scheme ERC-COG
 Starting year 2018
 Duration (year-month-day) from 2018-11-01   to  2023-10-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNIVERSITE DE LAUSANNE CH (LAUSANNE) coordinator 1˙999˙735.00

Map

 Project objective

Within clonal bacterial populations not all cells exhibit the same phenotype, even though they grow in the same environment. The molecular sources contributing to phenotypic variation are diverse and can originate from noise in gene expression to heterogeneity in growth rates or cell cycle state. Phenotypic variation helps pathogenic bacteria to elude the host immune response or resist antibiotic pressure. Vice versa, there is cell-to-cell variability in the host’s response towards pathogens that can be exploited by bacteria. How the combined cellular heterogeneity of both host and microbe contribute to infection outcome is poorly understood. The role of phenotypic variation on antibiotic resistance development is also unclear.

Recently, we developed novel single cell imaging systems as well as genetic engineering and screening platforms for application to the important opportunistic human pathogen Streptococcus pneumoniae. In addition, we generated a dual-transcriptomics overview of pneumococcal infection of human lung epithelial cells and setup collaborations to perform several infection models. This now places us in an excellent position to investigate the mechanisms and the importance of single cell behaviour for pneumococcal virulence and antibiotic resistance.

The driving hypothesis of this application is that the combined heterogeneity of host cells and pneumococci influences infection and antibiotic therapy outcome. To test this, we will use innovative approaches for infection biology by combining synthetic biology and quantitative single cell biology including single cell RNA-seq, CRISPRi, engineered bistable switches and microfluidics. We will reveal the molecular mechanisms underlying cell-to-cell variability and its importance in virulence and antibiotic resistance.

Insights obtained in this project will lead to a better understanding of phenotypic variation and might result in new treatment strategies for pneumococcal infections.

 Publications

year authors and title journal last update
List of publications.
2019 Jelle Slager, Rieza Aprianto, Jan-Willem Veening
Refining the Pneumococcal Competence Regulon by RNA Sequencing
published pages: , ISSN: 0021-9193, DOI: 10.1128/jb.00780-18
Journal of Bacteriology 201/13 2019-08-29
2019 Lance E. Keller, Anne-Stéphanie Rueff, Jun Kurushima, Jan-Willem Veening
Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniae
published pages: 394, ISSN: 2073-4425, DOI: 10.3390/genes10050394
Genes 10/5 2019-08-29
2019 Chryslène Mercy, Adrien Ducret, Jelle Slager, Jean-Pierre Lavergne, Céline Freton, Sathya Narayanan Nagarajan, Pierre Simon Garcia, Marie-Francoise Noirot-Gros, Nelly Dubarry, Julien Nourikyan, Jan-Willem Veening, Christophe Grangeasse
RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae
published pages: , ISSN: 2058-5276, DOI: 10.1038/s41564-019-0472-z
Nature Microbiology 2019-08-29

Are you the coordinator (or a participant) of this project? Plaese send me more information about the "PNEUMOCATCHER" project.

For instance: the website url (it has not provided by EU-opendata yet), the logo, a more detailed description of the project (in plain text as a rtf file or a word file), some pictures (as picture files, not embedded into any word file), twitter account, linkedin page, etc.

Send me an  email (fabio@fabiodisconzi.com) and I put them in your project's page as son as possible.

Thanks. And then put a link of this page into your project's website.

The information about "PNEUMOCATCHER" are provided by the European Opendata Portal: CORDIS opendata.

More projects from the same programme (H2020-EU.1.1.)

REPLAY_DMN (2019)

A theory of global memory systems

Read More  

E-DIRECT (2020)

Evolution of Direct Reciprocity in Complex Environments

Read More  

HYDROGEN (2019)

HighlY performing proton exchange membrane water electrolysers with reinforceD membRanes fOr efficient hydrogen GENeration

Read More