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Desiccation Survival SIGNED

Discovery of intrinsically disordered sequences conferring desiccation survival

Total Cost €

0

EC-Contrib. €

0

Partnership

0

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 Desiccation Survival project word cloud

Explore the words cloud of the Desiccation Survival project. It provides you a very rough idea of what is the project "Desiccation Survival" about.

unfolding    abundant    phylum    screening    tardigrades    protect    screen    transition    underlying    question    dry    proteins    animals    space    vitrified    unknown    confer    machine    sampling    aggregation    algorithms    disordered    aggregate    pipeline    periods    protective    throughput    sequence    extremely    paradigm    function    rescue    survive    intrinsically    protein    functions    generate    cahs    assay    perform    death    soluble    cytosolic    vivo    fundamental    validate    library    ing    materials    unravel    gelation    determinants    intracellular    organisms    micro    protection    dryness    extract    engineering    questions    heat    vitro    tested    cell    irreversibly    cells    shown    discover    idp    sequences    undergo    designing    family    unfold    aid    solids    form    idps    desiccation    rationally    biology    mediated    functional    learned    computational    stress    biomaterials    learning    glass    survival    hits   

Project "Desiccation Survival" data sheet

The following table provides information about the project.

Coordinator
UNITED KINGDOM RESEARCH AND INNOVATION 

Organization address
address: POLARIS HOUSE NORTH STAR AVENUE
city: SWINDON
postcode: SN2 1FL
website: n.a.

contact info
title: n.a.
name: n.a.
surname: n.a.
function: n.a.
email: n.a.
telephone: n.a.
fax: n.a.

 Coordinator Country United Kingdom [UK]
 Total cost 212˙933 €
 EC max contribution 212˙933 € (100%)
 Programme 1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility)
 Code Call H2020-MSCA-IF-2018
 Funding Scheme MSCA-IF-EF-ST
 Starting year 2019
 Duration (year-month-day) from 2019-04-01   to  2021-03-31

 Partnership

Take a look of project's partnership.

# participants  country  role  EC contrib. [€] 
1    UNITED KINGDOM RESEARCH AND INNOVATION UK (SWINDON) coordinator 212˙933.00

Map

 Project objective

Desiccation is a form of stress wherein extremely dry conditions cause intracellular proteins to unfold and aggregate irreversibly, resulting in cell-death. How do cells and organisms survive desiccation is a fundamental question in biology. Cytosolic Abundant Heat Soluble (CAHS) proteins, a family of intrinsically disordered proteins (IDPs) in tardigrades (a phylum of micro-animals), have been shown to be important for their survival during long periods of dryness. Under desiccation condition, CAHS proteins undergo glass-transition and gelation to form vitrified solids that protect intracellular proteins from unfolding and aggregation. However, the features of CAHS proteins that confer protection are unknown. Here, I aim to unravel the sequence determinants of CAHS protein functions, by addressing 3 specific questions:

Aim 1: What are the sequence features that promote glass-transition and gelation in CAHS proteins? Aim 2: Can we discover new sequences that can rescue cells from desiccation? Aim 3: What is the sequence-to-function paradigm underlying IDP-mediated desiccation survival?

I will (i) perform computational analysis of existing CAHS proteins to extract their sequence features to design a library for adequate sampling of the sequence space; (ii) screen the library with a high-throughput survival-based assay and validate the hits both in vitro and in vivo; (iii) analyse the results with machine learning algorithms to generate characteristic sequence features underlying protective glass-transition. The learned features will be tested by rationally designing and screening a new sequence library for desiccation survival. This project will provide fundamental sequence-level understanding of how IDPs promote stress response, specifically via glass-transition during desiccation. Moreover, the materials and pipeline generated and the findings of this study will aid in engineering functional biomaterials.

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The information about "DESICCATION SURVIVAL" are provided by the European Opendata Portal: CORDIS opendata.

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