Opendata, web and dolomites
Wall-E SIGNED
Make it, don’t break it: a reconstitution screen for Casparian strip formation in the root endodermis
Total Cost €
0EC-Contrib. €
0Partnership
0Views
0Wall-E project word cloud
Explore the words cloud of the Wall-E project. It provides you a very rough idea of what is the project "Wall-E" about.
limitations
nutrients
lethality
arabidopsis
mechanisms
mutant
cs
pioneering
redundancy
transmembrane
casparian
machinery
wp2
master
screen
combinatorial
dismutases
elucidate
screening
molecular
developmental
assembly
pioneer
organisms
laccases
super
generation
causing
coming
differentiating
model
reverse
enzymes
cell
formed
thirdly
activation
gain
function
slowly
standstill
uncover
endodermis
pleiotropy
oxide
stability
wp3
genes
activating
introduce
reconstitute
plants
genetically
decade
impregnations
dependent
functions
players
expressing
strips
firstly
differentiation
core
cells
efficiency
regulator
myb36
crispr
wp1
gene
genetic
lignin
activator
dirigent
selective
minimally
endodermal
sufficient
last
assessing
secondly
peroxidases
wall
attempt
proteins
cellular
polymerising
water
root
barrier
faced
Project "Wall-E" data sheet
The following table provides information about the project.
| Coordinator |
UNIVERSITE DE LAUSANNE
Organization address contact info |
| Coordinator Country | Switzerland [CH] |
| Total cost | 191˙149 € |
| EC max contribution | 191˙149 € (100%) |
| Programme |
1. H2020-EU.1.3.2. (Nurturing excellence by means of cross-border and cross-sector mobility) |
| Code Call | H2020-MSCA-IF-2019 |
| Funding Scheme | MSCA-IF-EF-ST |
| Starting year | 2021 |
| Duration (year-month-day) | from 2021-08-01 to 2023-07-31 |
Partnership
Take a look of project's partnership.
| # | ||||
|---|---|---|---|---|
| 1 | UNIVERSITE DE LAUSANNE | CH (LAUSANNE) | coordinator | 191˙149.00 |
Map
Project objective
In plants, the root endodermis functions as a barrier, allowing the selective uptake of nutrients and water. The barrier is formed by cell wall impregnations called Casparian strips (CS), produced by differentiating endodermal cells. In the last decade, pioneering work on the endodermis has identified numerous players involved in CS formation, including transmembrane proteins, peroxidases, dirigent-like proteins, lignin polymerising enzymes, laccases and super-oxide dismutases. However, the forward and reverse genetic approaches used to uncover these proteins are slowly coming to a standstill due to their limitations when faced with gene redundancy or genes with a broader range of activities, causing pleiotropy or lethality. I now propose to use CS formation as a model in order to pioneer a combinatorial, gain-of-function screen, aiming to define a minimally sufficient gene set for the assembly of a CS. I will attempt to reconstitute a CS in the endodermis of the myb36 mutant – a master regulator of endodermal differentiation - by activation screening for genes within the MYB36-dependent gene set. Firstly, I will introduce a “core machinery” for CS formation by expressing important known players and assessing CS formation and stability (WP1). Secondly, I will test the most recently developed second generation CRISPR activator systems for their efficiency in activating genes of interest in the endodermis (WP2). Thirdly, using the CRISPR activator technology, I will screen for novel genes that will improve the formation, stability and functionality of the CS (WP3). Identifying new genes involved in CS formation through a combinatorial, gain-of-function approach represents a novel way to genetically elucidate molecular mechanisms and could become a model for other cellular and developmental processes in Arabidopsis or other organisms.
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lastchecktime (2024-07-16 9:54:39) correctly updated