MEMSEMBLE

Assembling biomembranes: fundamentals of membrane transporter folding and creation of synthetic modules

Coordinatore	KING'S COLLEGE LONDON    more Spiacenti, non ci sono informazioni su questo coordinatore. Contattare Fabio per maggiori infomrazioni, grazie.
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	2˙312˙389 €
EC contributo	2˙312˙389 €
Programma	FP7-IDEAS-ERC Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	ERC-2011-ADG_20110310
Funding Scheme	ERC-AG
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-05-01   -   2017-04-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITY OF BRISTOL  Organization address address: TYNDALL AVENUE SENATE HOUSE city: BRISTOL postcode: BS8 1TH contact info Titolo: Ms. Nome: Sarah Cognome: Everett-Cox Email: send email Telefono: +44 117 9289678 Fax: +44 117 9298383	UK (BRISTOL)	beneficiary	566˙947.00
2	KING'S COLLEGE LONDON  Organization address address: Strand city: LONDON postcode: WC2R 2LS contact info Titolo: Mr. Nome: Paul Cognome: Labbett Email: send email Telefono: +44 2078488184 Fax: 442078000000	UK (LONDON)	hostInstitution	1˙745˙442.00
3	KING'S COLLEGE LONDON  Organization address address: Strand city: LONDON postcode: WC2R 2LS contact info Titolo: Prof. Nome: Paula Jane Cognome: Booth Email: send email Telefono: +44 20 7836 5454 Fax: 442078000000	UK (LONDON)	hostInstitution	1˙745˙442.00

Mappa

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 Obiettivo del progetto (Objective)

'Self-assembly is a hallmark of Biology. We are far from a complete understanding of this natural assembly, which in turn limits our ability to mimic biological construction in the bioengineering of tuneable synthetic systems. This proposal addresses the major challenge of membrane protein folding. Here, I intend to make a step change to my pioneering biophysical studies and investigate co-translational membrane protein folding. A central feature will be the creation of synthetic systems to probe key events in co-translational folding, as the protein folds in the membrane whilst emerging from the ribosome. An ambitious target is to make these systems tuneable, which will provide a new tool for the fabrication of membrane proteins and artificial cells in Synthetic Biology. The assembly of a tuneable artificial module that affords control over membrane protein synthesis is unprecedented. I focus on the ubiquitous superfamily of major facilitator proteins, namely the best studied family member, lactose permease, LacY. This proposal has state of the art biophysical mechanistic studies at its core, which interleave into Cell and Synthetic Biology. There are two themes: Theme 1. Determination of fundamental membrane protein folding parameters: folding transition states and lipid control Phi-value analysis will be used to probe the folding transition state of LacY; the first such analysis of a multi-domain membrane protein. Lipid parameters that control LacY folding will be quantified, including bilayer asymmetry using novel droplet interface bilayer methods. Theme 2: construction of tuneable synthetic co-translational folding systems Engineered ribosomes and translocon insertion machinery will be incorporated and LacY folding will be controlled. Translation will be regulated or halted using mutant ribosomes, arrest sequences, altered codon usage and controlling tRNA addition. Trapped LacY folding intermediates will be studied using biophysical methods.'