WOUND IN EARTH

Wound induced electrical signals in Arabidopsis thaliana

Coordinatore	JULIUS-MAXIMILIANS UNIVERSITAET WUERZBURG    more  Organization address address: SANDERRING 2 city: WUERZBURG postcode: 97070 contact info Titolo: Mr. Nome: Christian Cognome: Gloggengiesser Email: send email Telefono: +49 931 3182294 Fax: +49 931 3187180
Nazionalità Coordinatore	Germany [DE]
Totale costo	240˙455 €
EC contributo	240˙455 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2011-IIF
Funding Scheme	MC-IIF
Anno di inizio	2012
Periodo (anno-mese-giorno)	2012-05-01   -   2014-07-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	JULIUS-MAXIMILIANS UNIVERSITAET WUERZBURG  Organization address address: SANDERRING 2 city: WUERZBURG postcode: 97070 contact info Titolo: Mr. Nome: Christian Cognome: Gloggengiesser Email: send email Telefono: +49 931 3182294 Fax: +49 931 3187180	DE (WUERZBURG)	coordinator	74˙820.80
2	UNIVERSITE DE LAUSANNE  Organization address city: LAUSANNE postcode: 1015 contact info Titolo: Prof. Nome: Edward Cognome: Farmer Email: send email Telefono: +41 21 6924228 Fax: +41 21 6924195	CH (LAUSANNE)	participant	165˙634.27

Mappa

 Word cloud

Esplora la "nuvola delle parole (Word Cloud) per avere un'idea di massima del progetto.

 Obiettivo del progetto (Objective)

'Electrical phenomena in plants were first documented in the 1800s, yet progress in this area has been slow. There is evidence that most plants produce electrical signals that travel long distances within the plant. This electrical activity is triggered by biotic and abiotic stimuli, supporting the notion that excitability is a robust biological trait in plants. Major objectives of this project are to characterize waveform parameters (e.g. duration, amplitude) as well as dynamic parameters (e.g. velocity) of electrical signals induced by wounding in Arabidopsis thaliana. To this end, we will develop a technique that uses aphid stylets severed from feeding aphids with a highly focused radio-frequency pulse. With this approach we will register single electrical signals that travel along the phloem, a major site of electrical activity. These intracellular recordings will be paired with surface recordings obtained simultaneously for the same signals. Wounds will be inflicted with a collimated laser beam, an approach that does not mechanically disturb the plant and is therefore suitable for electrophysiology, while allowing controlling location and size of the wound. Laser wounding induces an electrical signal similar in waveform and duration to that induced by mechanical wounding (crushing). Both wounding methods dramatically increase expression of JAZ10, a jasmonate response gene that is commonly used as a marker of the wound response. The wound-induced electrical signal will be also investigated in loss-of-function mutant plants that either do not produce the jasmonate response (aos mutant) or that do not respond to jasmonate (coi1-1 mutant). Whether aphid infestation remodels the wound-induced electrical signal will also be tested. This is an interdisciplinary project that brings together an expert in the plant's wound response and an expert in animal electrophysiology, to create new knowledge in a neglected area of Biology.'