STREPSYNTH
Rewiring the Streptomyces cell factory for cost-effective production of biomolecules
| Coordinatore | KATHOLIEKE UNIVERSITEIT LEUVEN
Organization address
address: Oude Markt 13 contact info |
| Nazionalità Coordinatore | Belgium [BE] |
| Totale costo | 11˙330˙419 € |
| EC contributo | 8˙691˙070 € |
| Programma | FP7-KBBE
Specific Programme "Cooperation": Food, Agriculture and Biotechnology |
| Code Call | FP7-KBBE-2013-7-single-stage |
| Funding Scheme | CP-TP |
| Anno di inizio | 2013 |
| Periodo (anno-mese-giorno) | 2013-12-01 - 2018-11-30 |
Partecipanti
| # | ||||
|---|---|---|---|---|
| 1 |
KATHOLIEKE UNIVERSITEIT LEUVEN
Organization address
address: Oude Markt 13 contact info |
BE (LEUVEN) | coordinator | 1˙643˙099.00 |
| 2 |
Q-BIOLOGICALS NV
Organization address
address: TECHNOLOGIEPARK 4 contact info |
BE (GENT) | participant | 1˙042˙070.00 |
| 3 |
TECHNISCHE UNIVERSITAT BERLIN
Organization address
address: STRASSE DES 17 JUNI 135 contact info |
DE (BERLIN) | participant | 777˙550.00 |
| 4 |
EntreChem SL
Organization address
city: Oviedo contact info |
ES (Oviedo) | participant | 596˙450.00 |
| 5 |
HELMHOLTZ-ZENTRUM FUER INFEKTIONSFORSCHUNG GMBH
Organization address
address: Inhoffenstrasse 7 contact info |
DE (BRAUNSCHWEIG) | participant | 594˙794.00 |
| 6 |
UNIVERSITAET BIELEFELD
Organization address
address: UNIVERSITAETSSTRASSE 25 contact info |
DE (BIELEFELD) | participant | 578˙500.00 |
| 7 |
TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Organization address
address: TECHNION CITY - SENATE BUILDING contact info |
IL (HAIFA) | participant | 481˙650.00 |
| 8 |
NORGES TEKNISK-NATURVITENSKAPELIGEUNIVERSITET NTNU
Organization address
address: HOGSKOLERINGEN 1 contact info |
NO (TRONDHEIM) | participant | 481˙419.00 |
| 9 |
PROGENUS SA
Organization address
address: RUE DES PRAULES 2 contact info |
BE (GEMBLOUX) | participant | 463˙700.00 |
| 10 |
THE UNIVERSITY OF MANCHESTER
Organization address
address: OXFORD ROAD contact info |
UK (MANCHESTER) | participant | 320˙223.00 |
| 11 |
APRONEX S.R.O
Organization address
address: NAD SAFINOU II/365 contact info |
CZ (VESTEC JESENICE U PRAHY) | participant | 306˙450.00 |
| 12 |
UNIVERSITAT WIEN
Organization address
address: UNIVERSITATSRING 1 contact info |
AT (WIEN) | participant | 297˙186.00 |
| 13 |
MATIS OHF
Organization address
address: VINLANDSLEID 12 contact info |
IS (REYKJAVIK) | participant | 280˙290.00 |
| 14 |
FORSCHUNGSZENTRUM JUELICH GMBH
Organization address
address: Leo-Brandt-Strasse contact info |
DE (JUELICH) | participant | 264˙031.00 |
| 15 |
FOUNDATION FOR RESEARCH AND TECHNOLOGY HELLAS
Organization address
address: N PLASTIRA STR 100 contact info |
EL (HERAKLION) | participant | 251˙170.00 |
| 16 |
USTAV MOLEKULARNEJ BIOLOGIE SLOVENSKEJ AKADEMIE VIED
Organization address
address: DUBRAVSKA CESTA 21 contact info |
SK (BRATISLAVA) | participant | 183˙680.00 |
| 17 |
PHARMABIOTEC GMBH
Organization address
address: CAMPUS GEBAUDE C 2/2 UNIVERSITAT DES SAARLANDES contact info |
DE (SAARBRUECKEN) | participant | 128˙808.00 |
Mappa
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Obiettivo del progetto (Objective)
STREPSYNTH aims to set-up a Streptomyces-based new industrial production platform (SNIP) for high value added biomolecules. Streptomyces lividans was chosen as a bacterial host cell because it has been already shown to be highly efficient for the extracellular production of a number of heterologous molecules that vary chemically, has a robust tradition of industrial fermentation and is fully accessible to genetic intervention. To develop SNIP our strategy has two components: first, we will construct a collection of reduced-genome S. lividans strains. This will metabolically streamline the cell and rid it of agents (e.g. proteases) of potential harm to the heterologous polypeptides. Second, we will engineer synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements either indigenous to S. lividans or heterologous genes organized in artificial operon clusters. These elements will serve three aims: transcriptional and translational optimization, sophisticated on-demand transcriptional regulation that will provide unique fermentation control and metabolic engineering of complete cellular pathways channeling biomolecules to profuse extracellular secretion. Synthetic parts and cassettes will be either directly incorporated into the genome or be hosted in the form of plasmids. Systems biology tools will guide fine-tuning rounds of cell factory engineering and fermentation optimization. To set up SNIP we chose two classes of biomolecules with obvious immediate industrial value and application: heterologous proteins (industrial enzymes, biopharmaceuticals, biofuel enzymes, diagnostics) and small molecules (lantipeptides and indolocarbozoles) useful for multiple industrial purposes (biopharmaceuticals, additives, food technology, bioenergy). These biomolecules are of immediate interest to SMEs that participate and guide the industrial relevance of STREPSYNTH. SNIP is a modular platform that can be repurposed for diverse future applications.