HTSCS IN PLACENTA

Origin and lineage specification of trophoblast cells in early human placenta

Coordinatore	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE    more  Organization address address: The Old Schools, Trinity Lane city: CAMBRIDGE postcode: CB2 1TN contact info Titolo: Ms. Nome: Renata Cognome: Schaeffer Email: send email Telefono: +44 1223 333543 Fax: +44 1223 332988
Nazionalità Coordinatore	United Kingdom [UK]
Totale costo	231˙283 €
EC contributo	231˙283 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2013-IEF
Funding Scheme	MC-IEF
Anno di inizio	2014
Periodo (anno-mese-giorno)	2014-09-01   -   2016-08-31

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE  Organization address address: The Old Schools, Trinity Lane city: CAMBRIDGE postcode: CB2 1TN contact info Titolo: Ms. Nome: Renata Cognome: Schaeffer Email: send email Telefono: +44 1223 333543 Fax: +44 1223 332988	UK (CAMBRIDGE)	coordinator	231˙283.20

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 Obiettivo del progetto (Objective)

'My central question: how does the human placenta develop and how are specialized trophoblast sub-types generated? Despite advances in placental biology, pregnancy disorders occur frequently with few treatment options available, resulting in considerable maternal and/or infant mortality and morbidity. The cause of these disorders is abnormal early placental development but ethically and practically this is hard to investigate. Developing in vitro models that are truly representative of normal first trimester trophoblast will allow investigation of lineage specification and differentiation. I will take an interdisciplinary approach to characterize the first trimester villous cytotrophoblast (VCT) population. Specifically, I aim to: 1. Characterize the expression of trophoblast stem (TS) cell transcription factors and epigenetically regulated genes to identify the anatomical compartment(s) containing population(s) with stem cell characteristics 2. Isolate VCT using a specific cell surface marker and perform an in-depth molecular characterization at a single cell level to identify putative TS cells 3. Characterize cell signalling pathways in the VCT and in the putative TS cell population 4. Establish cell culture conditions for TS cells 5. Perform a detailed characterization of cells cultured in vitro This proposal has arisen from preliminary results obtained working in Centre for Trophoblast Research, University of Cambridge. Working in a lab with extensive experience of trophoblast isolation (Prof. A.Moffett) and input from our collaborators, Dr. M.Hemberger and Prof. G.Burton who have experience in mouse TS cells and human placental function in vivo, respectively has been pivotal. I am thus in an ideal position to capitalize on this expertise together with other stem cell labs in Cambridge to develop essential tools and knowledge to understand early developmental processes during placentation. It also has important translational impact for women and their babies.'