1CELL-MICROPROBE

Microfluidic electrochemical probes for both stimulation of single neuronal cells and real-time detection of inflammatory signalling compounds released from the cell

Coordinatore	UNIVERSITE LYON 1 CLAUDE BERNARD    more  Organization address address: BOULEVARD DU 11 NOVEMBRE 1918 NUM43 city: VILLEURBANNE CEDEX postcode: 69622 contact info Titolo: Dr. Nome: Javier Cognome: Olaiz Email: send email Telefono: +334 72 69 76 00 Fax: +334 72 69 76 09
Nazionalità Coordinatore	France [FR]
Totale costo	188˙250 €
EC contributo	188˙250 €
Programma	FP7-PEOPLE Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)
Code Call	FP7-PEOPLE-2007-4-1-IOF
Funding Scheme	MC-IOF
Anno di inizio	2008
Periodo (anno-mese-giorno)	2008-06-01   -   2010-11-30

 Partecipanti

#	participant	country	role	EC contrib. [€]
1	UNIVERSITE LYON 1 CLAUDE BERNARD  Organization address address: BOULEVARD DU 11 NOVEMBRE 1918 NUM43 city: VILLEURBANNE CEDEX postcode: 69622 contact info Titolo: Dr. Nome: Javier Cognome: Olaiz Email: send email Telefono: +334 72 69 76 00 Fax: +334 72 69 76 09	FR (VILLEURBANNE CEDEX)	coordinator	0.00

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 Obiettivo del progetto (Objective)

'Modern society, because of population ageing, suffers from a growing socio-economical impact of neurodegenerative diseases such as Alzheimer's, Parkinson's and Creutzfeldt-Jakob. Despite intense research efforts, the mechanisms responsible for the onset of these neuroinflammatory events, leading to neurodegeneration, are still unknown. Indeed, even if cytokines are well known to play a central role in the beginning of the neuroinflammatory events, their effects seem to depend on their localization and release time. Unfortunately, these local parameters, which are necessary to elucidate the neuroinflammatory mechanisms, are not accessible using traditional techniques. The aim of this project is to set-up an advanced, versatile, spatially and temporally controlled in vitro measurement tool for the electrochemical detection of signalling compounds released from a single cell. In order to reach the cellular temporal functionality and spatial dimension, we propose to use a microfluidic probe (MFP). The MFP operates by delivering a laminar stream of inducer solution through an opening (20 x 20 µm) and capturing it in a second opening, in a push-pull configuration. In the present project, microelectrodes will be added to the MFP, surrounding the apertures, in order to detect specifically the desired biomolecules in the cell micro-environment. These detections will be real-time and label-free electrochemical measurements (impedance or cyclic-voltammetry), based on the modification of the electrode surface behaviour, following biospecific interactions. The specificity of the detection will be achieved using immobilized antibodies at the electrode surface. After characterisation and improvement of the bio-sensing possibilities, the modified MFP will be used to both stimulate the cell and detect target compounds in the cell vicinity. This should allow us to characterize the intercellular signalling cascade and its kinetic properties.'

Introduzione (Teaser)

Thanks to the development by European scientists of a new miniature probe, observation and control of neurodegenerative diseases is coming of age.